Suggesting Tissue-Specific MSMB Gene Promoter as a Novel Approach for Prostate Targeted Gene Therapy

Document Type : Research Articles

Authors

1 Department of Molecular Medicine, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran.

2 Department of Pathology, Medical School of Shiraz University, Shiraz University of Medical Sciences, Shiraz, Iran.

3 Transplant Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.

4 Department of Medical Genetics, School of Medical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran.

5 Department of Hematology and Oncology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.

6 Hematology Research Centre, Shiraz University of Medical Sciences, Shiraz, Iran.

Abstract

Background and aim: Prostate cancer is the second most common cancer among men that has affected their quality of life. This study aimed to find prostate tissue-specific genes using bioinformatics methods to specifically target prostate cells in case of metastasis to other tissues. Materials and Methods: In this study, after finding a specific gene (MSMB)  that is highly expressed in cancer, the optimal promoter region of this gene was isolated and inserted in an expression vector. Then, this vector was transfected into two prostate cancer cell lines (DU145 and LNCaP) and three non-prostate cell lines  (LX-2, MRC-5, and U87) using the PEI chemical method. The expression of this vector in these cells was examined using fluorescent microscopy and flow cytometry. Results: We observed that the expression of MSMB promoter in DU145 cell line has a much higher activity than the CMV promoter, which is a ubiquitous promoter. The MSMB promoter didn’t show any activity in cells other than that of prostate derived cell lines. Conclusion: MSMB  gene promoter with specific expression and high efficiency in prostate tissue compared to CMV promoter can play an essential role in gene therapy of prostate cancer.

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Volume 23, Issue 6
June 2022
Pages 1993-2000
  • Receive Date: 17 January 2022
  • Revise Date: 25 March 2022
  • Accept Date: 11 June 2022
  • First Publish Date: 11 June 2022