The Effects of Umbilical Cord Mesenchymal Stem Cells -Derived Exosomes in Oral Squamous Cell Carcinoma (In vitro Study)

Document Type : Research Articles

Authors

1 Lecturer of Oral Medicine, Diagnosis and Periodontology, Faculty of Dentistry, October 6 University, Cairo, Egypt.

2 Lecturer of Oral and Maxillofacial Pathology, Faculty of Dentistry, October 6 University, Cairo, Egypt.

3 Department of Medical Biochemistry, Faculty of Medicine, Cairo University, Cairo, Egypt.

4 Department of Medical Biochemistry, Faculty of Medicine, Badr University in Cairo, Egypt.

Abstract

Objective: Mesenchymal stem cells (MSCs) derived exosomes offers several advantages as a cell-free therapeutic agents. In this study, Umbilical cord mesenchymal stem cells exosomes (UC-MSCs-exos) effects on oral squamous cell carcinoma (OSCC) cell line was evaluated. Methods: UC-MSCs-exos were isolated and co-cultured with OSCC cells and their impact on OSCC was explored by various tests. Comet assay and western blot for cleaved caspase-3 and immunocytochemistry for caspase-8 were used for apoptosis assessment. HO-1 and Nrf2 were used to determine antioxidant levels. Tumor necrosis factor-α and interleukin-6 were assessed as inflammatory biomarkers. HOX transcript antisense intergenic long noncoding RNA (HOTAIR) expression was also evaluated. Results: In a dose-dependent manner, UC-MSCs-exos reduced the levels of pro-inflammatory cytokines (IL-6 and TNF-α) and induced apoptosis of OSCC in vitro. Meanwhile, we found that UC-MSCs-exos downregulate HOTAIR. Conclusion: UC-MSCs-exos conferred a suppressive role on OSCC in vitro, highlighting a promising therapeutic role. However, the exact potentially involved molecules and molecular mechanisms need to be investigated in further studies.

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Asian Pacific Journal of Cancer Prevention
Volume 24, Issue 7
July 2023
Pages 2531-2542

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History

  • Receive Date: 25 April 2023
  • Revise Date: 29 May 2023
  • Accept Date: 08 July 2023

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