Low Frequency of Aflatoxin Induced TP53 Gene Codon 249 Mutation in Hepatocellular Carcinoma from Egyptian Patients Living in the Nile Delta Region

Mosbeh, Asmaa; Abdelmaguid, Waleed; Ezzat, Sameera; Kohla, Mohamed; Sultan, Mervat M; Abdel-Rahman, Mohamed H

doi:10.31557/APJCP.2023.24.9.3165

Low Frequency of Aflatoxin Induced TP53 Gene Codon 249 Mutation in Hepatocellular Carcinoma from Egyptian Patients Living in the Nile Delta Region

Document Type : Research Articles

Authors

Asmaa Mosbeh ¹^{, 2}
Waleed Abdelmaguid ²
Sameera Ezzat ³
Mohamed Kohla ⁴
Mervat M Sultan ¹
Mohamed H Abdel-Rahman ¹^{, 2}^{, 5}^{, 6}

¹ Department of Pathology, National Liver Institute, Menoufia University, Egypt.

² National Liver Institute Sustainable Sciences Institute Collaborative Research Center, Egypt.

³ Department of Public Health, National Liver Institute, Menoufia University, Egypt.

⁴ Department of Hepatology, National Liver Institute, Menoufia University, Egypt.

⁵ Department of Internal Medicine Division of Human Genetics and James Comprehensive Cancer Center, The Ohio State University Wexner Medical Center, Columbus, (OH), USA.

⁶ Department of Ophthalmology and Visual Sciences, The Ohio State University Wexner Medical Center, Columbus, (OH), USA.

10.31557/APJCP.2023.24.9.3165

Abstract

Objective: Study the frequency of codon 7 (c.747 G>T, p. R249S) mutation associated with Aflatoxin B1 (AFB1) exposure in Egyptian patients with hepatocellular carcinoma (HCC). Methods: We utilized restriction fragment polymorphism and direct sequencing to assess codon 7 mutations in 104 hepatocellular carcinomas. The expression of TP53 protein in the tumors were assessed in 44 tumors by a monoclonal rabbit antibody. Results: We identified a single 1/104 (1%) with c.747 G>T, p. R249S variant. 28/44 (63.6%) tumors showed no or occasional (less than < 5%) nuclear staining; 9/44 (20.4%) showed mild to moderate (5-49%) and 7/44 (15.9%) showed strong ≥ 50% staining. Conclusion: We observed much lower frequency of TP53 gene than previously published results suggesting geographical alterations in AFB1 exposure in Egypt.

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