Objective: This study aimed to determine whether microvessel density (MVD) in the tumor tissues could be a potential predictive marker for vascular invasion (VI). Methods: Surgical specimens of 73 patients with colorectal adenocarcinoma in Phramongkutklao Hospital were analyzed. Tissues of patients receiving preoperative radiation or prior anti-angiogenic therapy were excluded. Tumor MVD was determined using the average number of counted CD34-stained endothelial cells from two selected fields at 200x magnification in each slide. The presence of VI was defined by tumor involvement of endothelial cell-lined spaces. The optimal cut‐off value of MVD to predict VI was examined using receiver operating characteristic analysis to assess the area under the curve and accuracy. Result: VI was detected in 17 of 73 specimens (23.3%). Colorectal cancer (CRC) specimens were classified according to MVD as low (61 specimens, 83.6%) and high density (12 specimens, 16.4%). Average MVD was slightly higher in specimens with VI (81.3±9.3) than those without VI (76.3±7.6), but without statistical significance (p = 0.736). The MVD’s cut‐off value of 60 vessels/200x field provided 88% sensitivity, 40% specificity, and 57.5% accuracy, with the area under the ROC curve of 0.5788. Patients with CRC having MVD of > 60 vessels/200x field were at significantly higher risk of VI than those with CRC having MVD of <60 vessels/200x field (P=0.009, Fisher’s exact test). Univariate analysis revealed that MVD, nodal involvement and AJCC tumor stage were associated with the presence of VI (p <0.05). Further multivariate analysis of these three potential variables demonstrated MVD (OR, 11.994; 95% CI, 2.197 to 65.483; p <0.01) and nodal involvement (OR, 10.767; 95% CI, 1.973 to 58.748; p <0.05) as independent prognostic factors associated with VI. Conclusion: Based on our study, MVD immunostaining was an angiogenic marker that potentially be a predictive marker for VI.
Anannamcharoen, S., Boonya-ussadorn, C., & Nimmanon, T. (2023). Tissue Microvessel Density as a Potential Predictive Marker for Vascular Invasion in Colorectal Cancer. Asian Pacific Journal of Cancer Prevention, 24(12), 4097-4102. doi: 10.31557/APJCP.2023.24.12.4097
MLA
Sahaphol Anannamcharoen; Chinakrit Boonya-ussadorn; Thirayost Nimmanon. "Tissue Microvessel Density as a Potential Predictive Marker for Vascular Invasion in Colorectal Cancer". Asian Pacific Journal of Cancer Prevention, 24, 12, 2023, 4097-4102. doi: 10.31557/APJCP.2023.24.12.4097
HARVARD
Anannamcharoen, S., Boonya-ussadorn, C., Nimmanon, T. (2023). 'Tissue Microvessel Density as a Potential Predictive Marker for Vascular Invasion in Colorectal Cancer', Asian Pacific Journal of Cancer Prevention, 24(12), pp. 4097-4102. doi: 10.31557/APJCP.2023.24.12.4097
VANCOUVER
Anannamcharoen, S., Boonya-ussadorn, C., Nimmanon, T. Tissue Microvessel Density as a Potential Predictive Marker for Vascular Invasion in Colorectal Cancer. Asian Pacific Journal of Cancer Prevention, 2023; 24(12): 4097-4102. doi: 10.31557/APJCP.2023.24.12.4097
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