@article { author = {}, title = {H2O2 Inhibits Proliferation and Mediates Suppression of Migration via DLC1/RhoA Signaling in Cancer Cells}, journal = {Asian Pacific Journal of Cancer Prevention}, volume = {16}, number = {4}, pages = {1637-1642}, year = {2015}, publisher = {West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.}, issn = {1513-7368}, eissn = {2476-762X}, doi = {}, abstract = {Background: RhoGTPase-activating proteins (RhoGAPs) regulate RhoGTPases in cells, but whetherindividual reactive oxygen species (ROS) regulate RhoGAPs is unknown. Our previous published papers haveshown that deleted in liver cancer 1 (DLC1) inhibits cancer cell migration by its RhoGAP activity. The presentstudy was designed to explore the role of H2O2 in regulation of DLC1. Materials and Methods: We treated cells withH2O2 for 24h and phenotypic changes were analyzed by MTT, RT-PCR, Western blotting, immunofluorescencestaining and wound healing assays. Results: H2O2 downregulated cyclin D1 and cyclin E to inhibit proliferation,and upregulated BAX to induce apoptosis in MCF-7 cells. Compared with non-tumorigenic cells, H2O2 increasedexpression of DLC1 and reduced activity of RhoA in cancer cells. Stress fiber production and migration werealso suppressed by H2O2 in MDA-MB-231 cells. Conclusions: Our study suggests that H2O2 inhibits proliferationthrough modulation of cell cycle and apoptosis-related genes, and inhibits migration by decreasing stress fibersvia DLC1/RhoA signaling.}, keywords = {H2O2,Proliferation,deleted in liver cancer 1,RhoA,migration}, url = {https://journal.waocp.org/article_30631.html}, eprint = {https://journal.waocp.org/article_30631_45900ce54d7a311f854d7e9a2d2add4e.pdf} }