@article { author = {Sanaei, Masumeh and Kavoosi, Fraidoon and Dehghani, Faeze}, title = {Comparative Analysis of the Effects of 17-Beta Estradiol on Proliferation, and Apoptosis in Hepatocellular Carcinoma Hep G2 and LCL-PI 11 Cell Lines}, journal = {Asian Pacific Journal of Cancer Prevention}, volume = {19}, number = {9}, pages = {2637-2641}, year = {2018}, publisher = {West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.}, issn = {1513-7368}, eissn = {2476-762X}, doi = {10.22034/APJCP.2018.19.9.2637}, abstract = {Background: Phytoestrogens are a group of natural compounds with estrogen-like activity and similar structureto estradiol that structurally mimic the mammalian estrogen 17-β estradiol (E2). They have a biphasic effect and exertpleiotropic effects which induce or inhibit estrogen action by activation/inhibition of the estrogen receptors (ERs).These compounds can induce apoptosis at high concentrations. The previous finding indicated that E2 inhibited cellgrowth and induced apoptosis in hepatocellular carcinoma (HCC) PLC/PRF/5 cell line. The aim of the present studywas to investigate the apoptotic and proliferative effects of E2 on hepatocellular carcinoma HepG 2 and LCL-PI 11cells. Methods: The Hep G2 and LCL-PI 11 cells were cultured and treated with E2 for different time periods and thenMTT [3-(4, 5-dimethyl-2-thiazolyl) -2, 5-diphenyl -2H- tetrazolium bromide] assay and flow cytometry assay weredone to determine cell viability and cell apoptosis respectively. Results: E2 had inhibitory and apoptotic effects on HepG2 cell line, whereas it indicated a biphasic effect on LCL-PI 11 cell line. The half-maximum inhibitory concentration(IC50) value was 3 μM. The inhibitory effect of E2 on Hep G2 cells was observed with all concentrations of E2 (P<0.087), whereas E2 showed a biphasic effect on LCL-PI 11. This compound induced significant apoptosis in Hep G2cell line at the all treatment times versus control groups, whereas, in the LCL-PI 11 cell, significant apoptotic cells wereobserved after 72 and 96h (P <0.001). Conclusion: E2 can inhibit cell growth and induce apoptosis in hepatocellularcarcinoma HepG 2 and LCL-PI 11 cell lines.}, keywords = {Estradiol,viability,Apoptosis,Hepatocellular carcinoma}, url = {https://journal.waocp.org/article_69552.html}, eprint = {https://journal.waocp.org/article_69552_dcf90a2b4b38044814e57f615f01ad56.pdf} }