%0 Journal Article %T Anti-tumor Effects of pEgr-1-endostatin-TNF-α Recombinant Plasmid Expression Induced by Ionizing Radiation %J Asian Pacific Journal of Cancer Prevention %I West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter. %Z 1513-7368 %D 2011 %\ 11/01/2011 %V 12 %N 11 %P 2933-2937 %! Anti-tumor Effects of pEgr-1-endostatin-TNF-α Recombinant Plasmid Expression Induced by Ionizing Radiation %K Endostatin %K TNF-α %K gene-radiotherapy %K Irradiation %R %X Purpose: The aim of the present study was to investigate the anti-tumor effect of a pEgr-1-endostatin-TNF-α recombinant plasmid induced by ionizing radiation. Method: Three hundred and twenty mice bearing Lewis lung carcinomas were divided into four experimental groups: blank control, irradiation treatment, plasmid treatment and plasmid combined irradiation treatment. Twenty-four hours after the recombinant plasmid was injected locally into the tumors of the mice, they were irradiated with 10 Gy γ-rays. The concentration of TNF-α and endostatin in the serum of mice was measured by ELISA and tumor growth in each group was compared. The tumor microvessel density was examined by H&E staining and immunohistochemistry analysis of CD31 positive cells. Results: Radiation could induce the expression of pEgr-1-endostatin-TNFα. The levels of endostatin and TNF-α could express steadily for about 4 weeks, with concentrations of 52.6±4.19 and 12.0±0.87 ng/ml respectively, in the second week in combined therapy group and maintained at relative higher level in the fourth week than other groups (F=29.7, P<0.05). Compared with the control group, the tumor micro vessel density was significantly depressed (P<0.05) and tumor growth was significantly inhibited (5907.2±78.6 mm3 vs. 763.5±12.3 mm3, P <0.05). Conclusions: The expression of pEgr-1-endostatin-TNF-α could be induced in mice in vivo and exhibited more significant anti-tumor and anti-angiogenesis effects than irradiation alone. %U https://journal.waocp.org/article_25989_f2702b64c6c85b0e40ed133fdeccd735.pdf