%0 Journal Article %T Analysis of Hereditary Nonpolyposis Colorectal Cancer in Malay Cohorts using Immunohistochemical Screening %J Asian Pacific Journal of Cancer Prevention %I West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter. %Z 1513-7368 %D 2015 %\ 09/01/2015 %V 16 %N 9 %P 3767-3771 %! Analysis of Hereditary Nonpolyposis Colorectal Cancer in Malay Cohorts using Immunohistochemical Screening %K Colorectal carcinoma %K HNPCC %K Lynch syndrome %K immunohistochemistry %K MMR %R %X Background: Lynch syndrome (LS) is an inherited predisposition to colorectal, endometrial (uterine) andother cancers. Although most cancers are not inherited, about 5 percent (%) of people who have colorectal orendometrial cancer have the Lynch syndrome. It involves the alteration of mismatch repair (MMR) genes; MLH1,MSH2, MSH6 or PMS2. In this study, we analyzed the expression of MMR proteins in colorectal cancer in a Malaycohort by immunohistochemistry. Materials and Methods: A total of 17 patients were selected fulfilling one of theBethesda criteria: colorectal cancer diagnosed in a patient aged less than 50 years old, having synchronous andmetachronous colorectal cancer or with a strong family history. Immunohistochemical staining was performedon paraffin embedded tumour tissue samples using four antibodies: MLH1, MSH2, MSH6 and PMS2. Results:Twelve out of 17 patients (70.6%) were noted to have a family history. A total of 41% (n=7) of the patients hadabnormal immunohistochemical staining with one or more of the four antibodies. Loss of expression were notedin 13 tumour tissues with a negative staining score <4. Of 13 tumour tissues, four showed loss expression ofMLH1. For PMS2, loss of expression were noted in five cases. Both MSH2 and MSH6 showed loss of expressionin two tumour tissues respectively. Conclusions: Revised Bethesda criteria and immunohistochemical analysisconstituted a convenient approach and is recommended to be a first-line screening for Lynch syndrome in Malaycohorts. %U https://journal.waocp.org/article_30986_e7f233d601620d55905cb4a3ece95f7c.pdf