%0 Journal Article %T Anti-Proliferative Effects of Methanol and Water Extracts of Pyrrosia piloselloides on the Hela Human Cervical Carcinoma Cell Line %J Asian Pacific Journal of Cancer Prevention %I West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter. %Z 1513-7368 %A Sul ‘ain, Mohd Dasuki %A Zakaria, Fashihah %A Johan, Muhammad Farid %D 2019 %\ 01/01/2019 %V 20 %N 1 %P 185-192 %! Anti-Proliferative Effects of Methanol and Water Extracts of Pyrrosia piloselloides on the Hela Human Cervical Carcinoma Cell Line %K Pyrrosia piloselloides %K anti-proliferative %K Hela cell lines %R 10.31557/APJCP.2019.20.1.185 %X Background: Cervical cancer is one of the most commonly diagnosed neoplasms and a leading cause of cancerdeath among females worldwide. Limitations with conventional medical treatments have driven researchers tosearch for alternative approaches using natural products. This study aimed to detemine potential anti-proliferativeeffects of methanol and water extracts of Pyrrosia piloselloides (P. piloselloides) on the HeLa cell line. Methods:3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays were performed to determine IC50concentrations and apoptosis analysis was by flow cytometry. To identify chemical compounds in the extracts, gaschromatography-mass spectrometry (GC-MS) was employed. Results: P. piloselloides methanol extracts (PPME) showedantiproliferative effects on HeL awith an IC50 of 16.25μg/mL while the P. piloselloides water extract (PPWE) was withoutinfluence. Neither extract showed any significant effects on apoptosis. GC-MS analysis, revealed 5-hydroxymethylfurfural(23.1%), allopurinol (8.66%) and 3, 5-dihydroxy-6-methyl-2,3-dihydropyran-4-one (7.41%) as major components inthe PPME, while sulfolan-3-ol (10.1%), linoleic acid (9.06%) and β-sitosterol acetate (7.98%) predominated in thePPWE case. Conclusion: This first study of P. piloselloides showed PPME to exert potent anti-proliferative effect onHeLa cell lines. Further research now needs to be performed to establish the mechanisms of inhibition. %U https://journal.waocp.org/article_81711_da9eeafab87b5cdac3de9d48b1267356.pdf