%0 Journal Article %T Nannochloropsis Extract–Mediated Synthesis of Biogenic Silver Nanoparticles, Characterization and In Vitro Assessment of Antimicrobial, Antioxidant and Cytotoxic Activities %J Asian Pacific Journal of Cancer Prevention %I West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter. %Z 1513-7368 %A Gnanakani, Princely Ebenezer %A Santhanam, Perumal %A Premkumar, Kumpati %A Eswar Kumar, Kilari %A Dhanaraju, Magharla Dasaratha %D 2019 %\ 08/01/2019 %V 20 %N 8 %P 2353-2364 %! Nannochloropsis Extract–Mediated Synthesis of Biogenic Silver Nanoparticles, Characterization and In Vitro Assessment of Antimicrobial, Antioxidant and Cytotoxic Activities %K Nannochloropsis %K Silver nanoparticles %K Antioxidant activity %K Cytotoxic activity %R 10.31557/APJCP.2019.20.8.2353 %X Objective: To investigate the biogenic synthesis of silver nanoparticles (AgNPs) using partially purified ethylacetate extract of Nannochloropsis sp. hexane (EAENH) fraction of microalga. Methods: The green synthesis ofAgNPs was confirmed with UV-Vis spectrum which shows the surface plasmon resonance (SPR) at 421 nm. FourierTransform Infrared Spectra (FTIR) presented the involvement of functional groups like carboxyl groups of fatty acids,tetraterpenoids of xanthophylls, hydroxyl groups of polyphenols, carbonyl and amide linkage of proteins in the AgNPsynthesis. Gas Chromatography-Mass Spectrometry analysis (GCMS) revealed that phytochemicals like octadecanoicacid and hexadecanoic acid imply in capping, bioreduction, and stabilization of AgNps. Result: High-resolutionTransmission electron microscope (HRTEM), Dynamic light scattering (DLS), X-ray diffraction (XRD) and EDXanalysis showed the crystalline form of the AgNPs with Z-average size 57.25 nm. The zeta potential value of -25.7mV demonstrated the negative surface charge and colloidal stability of AgNPs. The antimicrobial activity of AgNPsdisplayed effective inhibition zone against selected bacterial and fungal pathogens. In vitro, antioxidant effects wereassessed by 1,1-diphenyl-2-picryl-hydrazyl (DPPH), hydrogen peroxide and reducing power assays which revealedexcellent scavenging potential for AgNPs than the extracts. The anti-proliferative potential of biofabricated AgNPsand extracts on Human Non-small lung cancer cell line (A549) was assessed using 3–(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay with IC50 values of 15 μgmL-1 and 175 μgmL-1 respectively. Conclusion:The study reveals that the microalgae-mediated AgNPs possesses potent antimicrobial and antioxidant activity alongwith the ability to stimulate apoptosis in A-549 cell line. %U https://journal.waocp.org/article_88683_49ca6c62184c83ae96c0ce5301099f87.pdf