%0 Journal Article %T Transcriptome and Network Dissection of Microsatellite Stable and Highly Instable Colorectal Cancer %J Asian Pacific Journal of Cancer Prevention %I West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter. %Z 1513-7368 %A Akbari, Vahid %A Kallhor, Marzieh %A Mollashahi, Behrouz %A Movafagh, Abolfazl %D 2019 %\ 08/01/2019 %V 20 %N 8 %P 2445-2454 %! Transcriptome and Network Dissection of Microsatellite Stable and Highly Instable Colorectal Cancer %K Biological processes %K Transcription factor %K FoxM1 %K DACH1 %K Tp53 %R 10.31557/APJCP.2019.20.8.2445 %X Background: Colorectal cancer (CRC) is one of the most common cancers worldwide with high number of mortalityevery year. Microsatellite instability (MSI) is a considerable feature of CRC which affects prognosis and treatment.High level of MSI or MSI-high (MSI-H) colorectal cancer has better prognosis and immunotherapy response, whilemicrosatellite stable (MSS) CRC has better response to 5-fluorouracil (5-FU)-based chemotherapy. More studies areneeded, specifically on MSS CRC which has worse prognosis, to further reveal biological differences and similaritiesbetween MSS and MSI colorectal cancer, which may equip us with the knowledge to develop more promising therapeuticapproaches to target both types or be more effective for each type. Methods: We aimed to find affected biologicalprocesses and their regulators in both type, MSS and MSI-H, of CRC; as well as reveal specific ones in each type.We applied meta- and network analysis on freely available transcriptome data in MSS and MSI-H colorectal cancerfrom gene expression omnibus (GEO) database to detect common differentially expressed genes (DEGs) and criticalbiological processes and predict their most significant regulators. Results: Our results demonstrate considerable upand downregulation in cell cycle and lipid catabolism processes, respectively; and introduced MYC and FOXM1as two central and up-stream regulators of DEGs in both type of CRC. Chemokine-mediated processes displayedup-regulation in MSI-H type, while metastasis-related processes showed more activation in MSS CRC. Additionally,DACH1 and TP53 were detected as two important transcription factors that differentially expressed just in MSS andMSI-H, respectively. Conclusion: Our results can explain why MSI and MSS CRC display different immunotherapyresponse, prognosis, and metastasis feature. Moreover, our predicted up-stream regulators in the regulatory networksmay be promising therapeutic targets. %U https://journal.waocp.org/article_88695_ec6ce05a4f8ab34f2ba629c25db2d2a7.pdf