TY - JOUR ID - 82546 TI - In Vitro Cytotoxic Activity of Clinacanthus nutans Leaf Extracts Against HeLa Cells JO - Asian Pacific Journal of Cancer Prevention JA - APJCP LA - en SN - 1513-7368 AU - Haron, Nor Hasyimah AU - Md Toha, Zaleha AU - Abas, Rafedah AU - Hamdan, Mohammad Razak AU - Azman, Nizuwan AU - Khairuddean, Melati AU - Arsad, Hasni AD - Advanced Medical and Dental Institute, Universiti Sains Malaysia, Bertam, 13200 Kepala Batas, Penang, Malaysia. AD - Centre for Drug Research, Penang, Malaysia. AD - School of Chemistry, Universiti Sains Malaysia, 11800 Minden, Penang, Malaysia. Y1 - 2019 PY - 2019 VL - 20 IS - 2 SP - 601 EP - 609 KW - Clinacanthus nutans KW - Phytochemical constituents KW - Antiproliferative KW - Apoptosis DO - 10.31557/APJCP.2019.20.2.601 N2 - Objective: This study was conducted to investigate the antiproliferative activity of extracts of Clinacanthus nutansleaves against human cervical cancer (HeLa) cells. Methods: C. nutans leaves were subjected to extraction using 80%methanol or water. The methanol extract was further extracted to obtain hexane, dichloromethane (DCM), and aqueousfractions. The antiproliferative activity of the extracts against HeLa cells was determined. The most cytotoxic extractwas furthered analyzed by apoptosis and cell cycle assays, and the phytochemical constituents were screened by gaschromatography-mass spectrometry (GC-MS). Results: All of the extracts were antiproliferative against HeLa cells, andthe DCM fraction had the lowest IC50 value of 70 μg/mL at 48 h. Microscopic studies showed that HeLa cells exposedto the DCM fraction exhibited marked morphological features of apoptosis. The flow cytometry study also confirmedthat the DCM fraction induced apoptosis in HeLa cells, with cell cycle arrest at the S phase. GC-MS analysis revealedthe presence of at least 28 compounds in the DCM fraction, most of which were fatty acids. Conclusion: The DCMfraction obtained using the extraction method described herein had a lower IC50 value than those reported in previousstudies that characterized the anticancer activity of C. nutans against HeLa cells. UR - https://journal.waocp.org/article_82546.html L1 - https://journal.waocp.org/article_82546_6320a03bfdb3eb04e6822cc17852ac8b.pdf ER -