TY - JOUR ID - 88643 TI - Evaluation of Protein Profiling in a Cohort of Egyptian Population with Renal Cell Carcinoma and Benign Kidney Neoplasms JO - Asian Pacific Journal of Cancer Prevention JA - APJCP LA - en SN - 1513-7368 AU - Kandil, Noha Said AU - Ghazala, Rasha Abdelmawla AU - El Sharkawy, Rania Mohamed AU - Youssif, Tamer Abou AU - Noha Abouseda, Noha AD - Department of Chemical Pathology, Medical Research Institute, Faculty of Medicine, Alexandria University, Egypt. AD - Department of Biochemistry, Faculty of Medicine, Alexandria University, Egypt. AD - Department of Urology, Faculty of Medicine, Alexandria University, Egypt. AD - Department of Microbiology, Faculty of Medicine, Alexandria University, Egypt. Y1 - 2019 PY - 2019 VL - 20 IS - 7 SP - 2145 EP - 2152 KW - Keywords: Magnetic Beads KW - MALDI TOF KW - Proteomic Profiling KW - renal cell carcinoma DO - 10.31557/APJCP.2019.20.7.2145 N2 - Abdominal imaging leads to the detection of a large number of renal tumors without the ability to distinguish thetype of tumor detected. It is necessary to find a precise way to know the type of tumor to determine the appropriatetreatment. The use of urine samples for detecting new biomarkers especially proteins has a great potential. In this workwe assessed the proteomic profiling difference in a cohort of Egyptian population with renal neoplasms. Methods:This cohort study was conducted on 85 subjects. They were classified as 40 RCC, 15 benign kidney patients, and 30healthy controls. Morning urine samples were used for peptidome separation using magnetic beads. Matrix assisted laserdesorption ionization time of flight mass spectrometry (MALDI-TOF MS) was applied Using FlexControlTM software.Results: Benign tumors were differentiated from controls by 5 integrated peaks, 12 significant and 2 integrated significantpeaks, 17:3,418.8 and 25:4,173.41. While RCC were differentiated from benign by 5 integrated, 28 significant and oneintegrated significant peak. The RCC group was discriminated from the controls by 5 peaks which were integrated fromwhich 1 was integrated and significant (with mass to charge ratio of 12:3,408.97). The three groups showed proteinprofiles ranging from 1 to 10 kDa. The external validation was performed for the RCC group versus the control reveledsensitivity of 88.7% and specificity of 73.2% by genetic algorithm. Conclusion: Proteomic approach can be used as asensitive urinary marker differentiating renal masses in an early diagnostic approach. UR - https://journal.waocp.org/article_88643.html L1 - https://journal.waocp.org/article_88643_8dc3e4020b5ae92f5a83cda5fc43934e.pdf ER -