TY - JOUR ID - 88649 TI - Anticancer Activities of Ricin-Liposome Complexes on SKMEL-28 Cells JO - Asian Pacific Journal of Cancer Prevention JA - APJCP LA - en SN - 1513-7368 AU - Bich Loan, Nguyen Thi AU - Trung, Ngo Ngoc AU - Le Na, Nguyen Thi AU - Thang, Nguyen Dinh AD - Department of Biochemistry and Molecular Biology, VNU University of Science, Vietnam National University, Vietnam. AD - Institute of Chemistry and Environment, High Command of Chemistry, Ministry of National Defense, Vietnam. Y1 - 2019 PY - 2019 VL - 20 IS - 7 SP - 2117 EP - 2123 KW - ricin KW - migration KW - Invasion KW - Tumorigenesis KW - Melanoma DO - 10.31557/APJCP.2019.20.7.2117 N2 - Background: Ricin has been reported as a potential chemical for cancer treatment. However, so far, the applicationof ricin in cancer treatment is very limited because of its non-specificity. Methods: In this study, ricin were conjugated/encapsulated with DOTAP/DOPE liposome to form ricin-liposome complexes (ricin-lipososme1, ricin-liposome2,ricin-liposome3 and ricin-liposome4). Characteristics of ricin-liposome complexes were analyzed and their effects onsurvival, apoptosis, migration, invasion and tumor formation of SKMEL-28 melanoma cells were examined by carryingout the MTT assay, apoptosis assay, scratch wound healing assay, invasion assay and soft-agar colony formation assay,respectively. Results: Ricin-liposome complexes had even size-distribution with average size of around 340 nm. Thesericin-liposome complexes were able to penetrate into the cells via endocytosis with the highest ability of the ricinliposome3.It also showed that ricin-liposome3 expressed very high toxicity with the IC50 of 62.4 ng/mL and followedby ricin-liposome4 (286.4 mg/mL), ricin-liposome2 (417.5 ng/mL), and ricin-liposome1 (604.3 ng/mL) to SKMEL-28cells at 36 hours post treatment. At the concentrations of IC10 (10.1 ng/mL), ricin-liposome3 strongly induced necrosisand apoptosis of SKMEL-28 cells up to 25.6% and 11.4%, respectively. Moreover, ricin-liposome3 expressed greatanticancer properties by decreasing the migration, invasion and tumor formation abilities of SKMEL-28 cells of 7.5folds, 4.3 folds and 5.9 folds, respectively, compared with those of control SKMEL-28 cells. Conclusion: The obtainedresults from our study suggest that although ricin is listed as one of the most poisonous substances in nature, it can beused in the complex forms with liposome to increase its specificity to apply in treatment of melanoma and other cancers. UR - https://journal.waocp.org/article_88649.html L1 - https://journal.waocp.org/article_88649_73cfd9a1f61519d0ea8bb487c7669e3d.pdf ER -