TY - JOUR ID - 88760 TI - Fecal Genetic Mutations and Human DNA in Colorectal Cancer and Polyps Patients JO - Asian Pacific Journal of Cancer Prevention JA - APJCP LA - en SN - 1513-7368 AU - Lima, Jacqueline AU - Teixeira, Yolanda AU - Pimenta, Célia AU - Felipe, Aledson Vitor AU - Silva, Tiago Donizetti AU - Junior, Ermelindo Ermelindo Della Libera AU - Saad, Sarhan Sydney AU - Deak, Elisabeth AU - Murray, Helena AU - Manoukian Forones, Nora AD - Oncology Group, Gastroenterology Division, Medicine Department, Universidade Federal de Sao Paulo, São Paulo, Brazil. AD - Gastroenterology Division and Endoscopist, Universidade Federal de Sao Paulo, São Paulo, Brazil. AD - Surgery Department, Universidade Federal de Sao Paulo, São Paulo, Brazil. AD - Randox Laboratories Ltd., Crumlin, Co. Antrim, United Kingdom. Y1 - 2019 PY - 2019 VL - 20 IS - 10 SP - 2929 EP - 2934 KW - Stool DNA KW - colorectal cancer KW - Polyps KW - colonoscopy KW - Screening DO - 10.31557/APJCP.2019.20.10.2929 N2 - Background: Colorectal cancer (CRC) is one of the most frequent cancers. Genetic mutations in CRC already described can be detected in feces. Microarray methods in feces can represent a new diagnostic tool for CRC and significant improvement at public health. Aim: to analyze stool DNA by human DNA quantify and microarray methods as alternatives to CRC screening. Method: Three methods were analyzed in stool samples: Human DNA Quantify, RanplexCRC and KRAS/BRAF/PIK3CA (KBP) Arrays. Results: KBP array mutations were presented in 60.7% of CRC patients and RanplexCRC Array mutations in 61.1% of CRC patients. Sensitivity and specificity for human DNA quantification was 66% and 82% respectively. Fecal KBP Array had 35% sensitivity and 96% specificity and RanplexCRC Array method had 78% sensitivity and 100% specificity. Conclusion: Microarray methods showed promise as potential biomarkers for CRC screening; however, these methods had to be optimized to improve accuracy and applicability by clinical routine. UR - https://journal.waocp.org/article_88760.html L1 - https://journal.waocp.org/article_88760_7da7aca6b155735e8c4a021f473498ce.pdf ER -