TY - JOUR ID - 90300 TI - Interleukin-10-1082A>G (rs1800896) Single Nucleotide Polymorphism is Not a Risk Factor of Chronic Lymphocytic Leukemia in Sudanese Population JO - Asian Pacific Journal of Cancer Prevention JA - APJCP LA - en SN - 1513-7368 AU - Basabaeen, Ameen Abdulaziz AU - Abdelgader, Enaam Abdelrhman AU - Ahmed, Ebtihal Babekir AU - Abdelateif, Nour Mahmoud AU - Osman Abdelrahim, Sadia AU - Sharif, Omnia Mohamed AU - Altayeb, Osama Ali AU - Fadul, Eman Abbass AU - Osman Ibrahim, Ibrahim AD - Department of Hematology, Faculty of Medical Laboratory Sciences, Al Neelain University, Khartoum, Sudan. AD - Department of Pathology, Faculty of Medicine, Al Neelain University, Khartoum, Sudan. AD - Department of Hematology, Faculty of Medical Laboratory Sciences, Al Neelain University, Khartoum, Sudan. AD - Department of Haematology, Faculty of Medical Laboratory Sciences, Sudan University of Science and Technology, Khartoum, Sudan. AD - Flow Cytometry Laboratory for Leukemia & Lymphoma Diagnosis, Khartoum, Sudan. Y1 - 2022 PY - 2022 VL - 23 IS - 9 SP - 3229 EP - 3235 KW - CLL KW - Interleukin-10 KW - Single nucleotide polymorphism KW - IL-1082A> G DO - 10.31557/APJCP.2022.23.9.3229 N2 - Objective: The present study was conducted to examine the association between the IL-10-1082A>G (rs 1800896) polymorphism and risk of Chronic Lymphocytic Leukemia and to assess the correlation between this polymorphism and clinicopathological characters. Methods: A case-control study was conducted in Khartoum state, Sudan, during the period from April 2017 to April 2018, involved 110 CLL patients and 80 healthy volunteers as a control group. Physical examination, complete blood count, and immunophenotype were performed in all patients to confirm the diagnosis. Clinical staging such as Rai and Binet were studied. CD38 and ZAP70 were performed by flow cytometry. Blood samples were collected from all participants; DNA was extracted by using ANALYTIKJENA Blood DNA Extraction Kit and analyzed IL-10-1082A>G polymorphism by using Allele Specific-Polymerase Chain Reaction. The statistical analysis was performed using statistical  package  for  social  sciences  version 23.0 software. Results: Frequency of AA, AG, and GG genotypes was 32.7%, 55.5%, and 11.8% for the patient group and 31.25%, 51.25%, and 17.5% in the control group, respectively. The genotype of IL-10 (-1082A>G) did not associate with susceptibility of CLL in our population. The study showed that the G allele of the IL-10 gene (-1082A>G) is associated with the male sex. However, no significant association was found between -1082A>G genotype and clinicopathological characters. Conclusion: Our results do not support the involvement of the IL-10 −1082A>G promoter gene polymorphism in the increased CLL susceptibility. IL-10-1082G allele (IL-10-1082AG or IL-10-1082GG) was found more frequently in males. Furthermore, no association was observed between the IL-10-1082A>G polymorphism and clinical stages systems as well as established poor prognostic markers. Finally, within the group of patients with CLL, there was no difference in the age at diagnosis and hematological parameters according to genotype distributions. UR - https://journal.waocp.org/article_90300.html L1 - https://journal.waocp.org/article_90300_2a7b760b34ddff7df92f5f8cd67b6afe.pdf ER -