West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter. Asian Pacific Journal of Cancer Prevention 1513-7368 17 6 2016 07 01 Hypermethylation Status of E-Cadherin Gene in Gastric Cancer Patients in a High Incidence Area 2757 2760 16302 APJCP.2016.17.6.2757 EN Haroon Rashid Department of General and minimal invasive surgery, Sher-I- Kashmir Institute of Medical Sciences, Soura, Srinagar, Kashmir, India Haroon Rashid Department of General and Minimal Invasive Surgery,Soura, Srinagar, Kashmir, India Equal contributors Khursheed Alam Department of General and Minimal Invasive Surgery,Soura, Srinagar, Kashmir, India Equal contributors Dil Afroze Department of Immunology and Molecular Medicine, Soura, Srinagar, Kashmir, India Adfar Yousuf Department of Immunology and Molecular Medicine, Soura, Srinagar, Kashmir, India Manzoor Banday Department of Medical Oncology, Sher-I-Kashmir Institute of Medical Sciences, Soura, Srinagar, Kashmir, India Fizalah Kawoosa Department of Immunology and Molecular Medicine, Soura, Srinagar, Kashmir, India Journal Article 1970 01 01 Gastric cancer (GC) is the fourth most prevalant cancer and the second leading cause of cancer-related mortality worldwide. As in other cancers gastric carcinogenesis is multifactorial involving environmental, genetic and epigenetic components. Epigenetic silencing due to hypermethylation of tumour suppressor genes is one of the key events in gastric carcinogenesis. This study was aimed to analyse the hypermethylation status of the E-Cadherin (<em>CDH1</em>) gene promoter in GCs in the ethnic Kashmiri population. In this study a total of 80 GC patients were recruited. Hypermethylation in tumour tissue was detected by methylation specific PCR (MS-PCR). Hypermethylation of <em>CDH1 </em>promoter was observed in 52 (65%) of gastric carcinoma cases which was significantly much higher than adjacent normal tissue [p≤0.0001]. Further the frequency of <em>CDH1 </em>promoter methylation was significantly different with intestinal and diffuse types of gastric cancer [55.7% vs 82.1%; p<0.05]. Moreover females and cases with lymph node invasion had higher frequencies of <em>CDH1 </em>hypermethylation [P≤0.05]. Thus the current data indicate a vital role of epigenetic alteration of <em>CDH1 </em>in the causation and development of gastric cancer, particularly of diffuse type, in our population. https://journal.waocp.org/article_16302_835e680f24a3d5ea65a282cce99893c8.pdf