West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter. Asian Pacific Journal of Cancer Prevention 1513-7368 10 4 2009 04 01 Pharmacological Activity of Kaempferia parviflora Extract against Human Bile Duct Cancer Cell Lines 695 698 24992 EN Journal Article 1970 01 01 crude ethanol extract of Kaemperia parviflora Wall. Ex Baker and a purified compound, 5,7,4-trimethoxyflavone (KP.8.10), were evaluated for pharmacological effects on human cholangiocarcinoma celllines (HuCCA-1 and RMCCA-1). The cells were incubated with various concentrations of extract for varioustime periods and metabolic activity (MTT assay) was assessed for cell viability. The results showed a dosedependenteffect of both crude ethanol extract and the pure compound. CC50s for the crude extract on HuCCA-1 and RMCCA-1 cells were 46.1μg/ml and 62.0μg/ml, respectively. Values for the pure compound could not bedetermined because of solubility problems. Interestingly, K. parviflora ethanol extract and KP.8.10 at lowconcentrations (10-20μg/ml and 2.5-5 μg/ml, respectively) markedly reduced rhHGF-induced invasion byHuCCA-1 and RMCCA-1 cells across matrix-coated transwell plates. Higher concentrations of K. parvifloraethanol extract (60 and 80μg/ml) and KP.8.10 (20 μg /ml) dramatically changed the cellular morphology andcaused death in both cell types. KP.8.10 further exhibited progressive action via caspase-3 mitochondrial enzymeactivation, enhancing cellular toxicity in a time-dose dependent fashion. Therefore, 5,7,4-trimethoxyflavoneappeared to be a bioactive component of K. parviflora extract capable of exerting anti-cancer action. The resultssuggested a benefit of this edible plant in prevention and treatment of cholangiocarcinoma. https://journal.waocp.org/article_24992_4dce180e08db1117827942ee0cf4f49c.pdf