West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter. Asian Pacific Journal of Cancer Prevention 1513-7368 10 6 2009 06 01 Punarnavine Induces Apoptosis in B16F-10 Melanoma Cells by Inhibiting NF-kB Signaling 1031 1032 25053 EN Journal Article 1970 01 01 The objective of this study was to assess the effect of Punarnavine, an alkaloid isolated from Boerhaaviadiffusa, on apoptosis in B16F-10 melanoma cells. Treatment of B16F-10 melanoma cells with nontoxicconcentrations of Punarnvine resulted in the presence of apoptotic bodies and DNA fragmentation in a dosedependent manner. Cell cycle analysis and TUNEL assays also confirmed the observation. The apoptotic genesp53 and caspase-3 were found upregulated in Punarnavine treated cells, whereas the antiapoptotic gene Bcl-2was downregulated. The inhibited nuclear translocation of NF-κBp65, NF-κBp50, NF-κB-c-Rel, c-Fos, ATF-2and CREB-1 in Punarnavine treated B16 F-10 cells pointed to suppression of NF-κB signaling by Punarnavine.All these results demonstrate that Punarnavine induces apoptosis via activation of p53 induced caspase-3 mediatedpro-apoptotic signaling and suppression of NF-κB induced Bcl-2 mediated survival signaling. https://journal.waocp.org/article_25053_3ac04b71f674c365174f67511fc41e55.pdf