West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter. Asian Pacific Journal of Cancer Prevention 1513-7368 12 4 2011 04 01 Her2 Amplification Status in Iranian Breast Cancer Patients: Comparison of Immunohistochemistry (IHC) and Fluorescence in situ Hybridisation (FISH) 1031 1034 25652 EN Journal Article 1970 01 01 Introduction: Her2/neu is a biomarker which is amplified and/or overexpressed in a subset of breast cancerpatients who are eligible to receive trastuzumab. Her-2 gene amplification analysed by fluorescence in situhybridisation (FISH) and/or protein over-expression detected by immunohistochemistry (IHC) are the two mainmethods used to detect Her-2 status in clinical practice. The concordance rate between the two techniques iscontroversial. Methods: FISH analysis were performed on 104 tumoural samples from breast cancer patientswith known IHC results to determine the Her2 gene status. The FISH/IHC analyses results were then comparedand the concordance rate was determined. Results: Her2 gene amplification was detected in 0 of IHC score 1+,24/86 (27.91%) 2+, and 8/13 (61.54%) 3+. The IHC and FISH results concordance rates were 100%, 27.9%,and 61.5% for IHC scores of 1+, 2+, and 3+ respectively. Conclusion: The results of this study suggest that IHC1+ should be considered as negative while IHC 2+ results need further confirmative analysis by FISH. Furtherquality control and standardization of IHC technique are required to improve the concordance rate betweenthe two methods.

https://journal.waocp.org/article_25652_f0d4f991f81a77b0fb2a8f1e674557bd.pdf