West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter. Asian Pacific Journal of Cancer Prevention 1513-7368 13 5 2012 05 01 Mechanism of P-glycoprotein Expression in the SGC7901 Human Gastric Adenocarcinoma Cell Line Induced by Cyclooxygenase-2 2379 2383 26480 EN Journal Article 1970 01 01 <br/><b>Objective</b>: To investigate possible signal pathway involvement in multi-drug resistant P-glycoprotein (P-gp)expression induced by cyclooxygenase-2 (COX-2) in a human gastric adenocarcinoma cell line stimulated withpacliaxel (TAX). <br/><b>Methods</b>: The effects of TAX on SGC7901 cell growth with different doses was assessed byMTT assay, along with the effects of the COX-2 selective inhibitor NS-398 and the nuclear factor-ΚB (NF-ΚB)pathway inhibitor pyrrolidine dithiocarbamate (PDTC). Influence on COX-2, NF-ΚB p65 and P-gp expressionwas determined by Western blotting. <br/><b>Results</b>: TAX, NS-398 and PDTC all reduced SGC7901 growth, with dosedependence.With increasing dose of TAX, the expression of COX-2, p65 and P-gp showed rising trends, thisbeing reversed by NS-398. PDTC also caused decrease in expression of p65 and P-gp over time. <br/><b>Conclusion</b>:COX-2 may induce the expression of P-gp in SGC7901 cell line via the NF-kappa B pathway with pacliaxelstimulation. https://journal.waocp.org/article_26480_e895329ba18a0ccc71fa444bcf74096d.pdf