West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter. Asian Pacific Journal of Cancer Prevention 1513-7368 14 7 2013 07 01 Biomarkers Screening Between Preoperative and Postoperative Patients in Pancreatic Cancer 4161 4165 27924 EN Journal Article 1970 01 01 Objective: To investigate discriminating protein patterns and potential biomarkers in serum samples betweenpre/postoperative pancreatic cancer patients and healthy controls. Methods: 23 serum samples from PC patients(12 preoperative and 11 postoperative) and 76 from healthy controls were analyzed using matrix-assisted laserdesorption and ionization time-of-flight mass spectrometry (MALDI-TOF MS) technique combined with magneticbeads-based weak cation-exchange chromatography (MB-WCX). ClinProTools software selected several markersthat made a distinction between pancreatic cancer patients and healthy controls. Results: 49 m/z distinctive peakswere found among the three groups, of which 33 significant peaks with a P < 0.001 were detected. Two proteinscould distinguish the preoperative pancreatic cancer patients from the healthy controls. About 15 proteins maybe potential biomarkers in assessment of pancreatic cancer resection. Conclusion: MB-MALDI-TOF-MS methodcould generate serum peptidome profiles of pancreatic cancer and provide a new approach to identify potentialbiomarkers for diagnosis and prognosis of this malignancy.

https://journal.waocp.org/article_27924_b327e8feceaf46b0ef0d69c4a71ab4f0.pdf