West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter. Asian Pacific Journal of Cancer Prevention 1513-7368 18 1 2017 01 01 Enhancing Effects of Curcumin on Cytotoxicity of Paclitaxel, Methotrexate and Vincristine in Gastric Cancer Cells 65 68 43090 10.22034/APJCP.2017.18.1.65 EN Meysam Ebrahimifar Department of Toxicology, Faculty of Pharmacy, Islamic Azad University, Shahreza Branch, Shahreza Majid Hasanzadegan Roudsari Department of chemical Engineering, Science and Research branch, Islamic Azad University, Tehran, Iran. S Maryam Kazemi Department of Genetics, Islamic Azad University, Tehran Medical Branch, Tehran, Iran Hasan Ebrahimi Shahmabadi Department of Microbiology, Rafsanjan University of Medical Sciences, Rafsanjan, Iran. Leila Kanaani Department of Toxicology, Faculty of Pharmacy, Islamic Azad University, Shahreza Branch, Shahreza S.A. Alavi Hakiman-e-Shargh Investigative Corporation, Isfahan Science and Technology Town, Isfahan, Iran Mehdi Izadi Vasfi Department of Chemical Engineering, Faculty of Pharmacy, Islamic Azad University, Shahreza, Iran Journal Article 2016 09 20 Curcumin (Diferuloylmethane), a polyphenolic compound with antioxidant, anti-inflammatory and anticancer properties, has been found to increase chemotherapeutic agents-induced cytotoxicity in some resistant cancer cell lines. This investigation aimed to study the effects of curcumin on efficacy of some common anticancer agents in gastric cancer cells. AGS cells were cultured in RPMI-1640 medium under standard culture conditions (5% CO2 and 95% humidified air at 37°C). Curcumin was used at concentrations of 5, 15, 30 and 50 μM. Cells were treated with a combination of curcumin and paclitaxel (300 nm) or methotrexate (100 μm) or vincristine (5 nm). Cell viability, the percentage of live cells in the whole population, was evaluated by MTT assay after 48 hours. The results showed that cell viability was significantly decreased after incubation of AGS cells with curcumin. Combination with curcumin (15-50 μm) significantly increased cytotoxicity of all three agents (P<0.001). Regarding high anticancer potential and enhancement of chemotherapeutic agent-induced cytotoxicity, the combined use of curcumin with standard chemotherapy of gastric cancer is suggested as a strategy for better management of this fatal cancer.

https://journal.waocp.org/article_43090_6d1566ef8145ed2d812776822ff02094.pdf