West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter. Asian Pacific Journal of Cancer Prevention 1513-7368 18 1 2017 01 01 Association of Some Polymorphisms in the VDR Gene, CYP17 Gene and SRD5A2 Gene and Prostate Cancer among Lebanese Men 93 100 43109 10.22034/APJCP.2017.18.1.93 EN Asmahan El Ezzi Lebanese Atomic Energy Commission, Beirut, Lebanon Department of Biochemistry, Lebanese University, Hadath, Lebanon Department of Chemistry, Utah Valley University, Orem, UT, USA. Vladyslav G Boyko Department of Biology, Utah Valley University, Orem, UT, USA Monika Baker Department of Biology, Utah Valley University, Orem, UT, USA Wissam R Zaidan Lebanese Atomic Energy Commission, Beirut, Lebanon Kalim M Hraiki Department of Urology, Al-Salam Hospital, Tripoli, Lebanon Mohammad A El-Saidi Office of Academic Research Support, Utah Valley University, Orem, Utah, USA Ruhul Kuddus Department of Biology, Utah Valley University, Orem, UT, USA 0000-0003-0697-1628 Journal Article 2016 10 21 Aims: The goal of the study was to investigate possible association of some single nucleotide polymorphisms<br />(SNPs) in the VDR gene (the FokI, BsmI, ApaI and TaqαI loci), and the CYP17 gene (the MspA1I locus), and 0 or 9<br />TA repeats in the SRD5A2 gene, and prostate cancer (PCa) among Lebanese men. Materials and Methods: Blood<br />DNA of 69 subjects with confirmed PCa and 69 controls, all about 50 years of age or older, was subjected to PCR or<br />PCR-restriction fragment-length polymorphism (PCR-RFLP) analyses, and the risk-bearing and the protective alleles<br />were identified. The odds ratio (OR) of having a genotype and the relative risk (RR) of developing PCa were calculated.<br />In addition, the distributions of homozygosis and heterozygosis in the risk-bearing alleles and the protective alleles<br />among the control and the PCa groups were compared. Results: The f allele of the VDR FokI locus and the (TA) 9<br />repeat allele of the SRD5A2 gene were found to be associated with increased risks of PCa (p = 0.006 and 0.050,<br />respectively). Homozygosis in the risk-bearing alleles was rare both in the control and the PCa groups. A higher<br />fraction of the controls compared to the PCa group was double-homozygous in the two protective alleles (52.2% for<br />controls, 24.6% for PCa group, p = <0.001). Conclusions: To the best of our knowledge, this is the first genetic study<br />demonstrating the association of certain polymorphisms of the VDR gene and the SDR5A2 gene and increased risk of<br />PCa among Lebanese men. Our study also indicates that the overall polymorphism profile of all genes involved in<br />the prostate physiology is likely to be a better indicator for PCa risk than the polymorphisms in the individual genes. https://journal.waocp.org/article_43109_c4bd43f730dab08288a87be2ce4b1b92.pdf