West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter. Asian Pacific Journal of Cancer Prevention 1513-7368 19 5 2018 05 01 Radiotherapy Enhancement with Electroporation in Human Intestinal Colon Cancer HT-29 Cells 1259 1262 60721 10.22034/APJCP.2018.19.5.1259 EN Ali Yadollahpour Department of Medical Physics, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. Zohre Rezaee Department of Medical Physics, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. Vahid Bayati Cellular and Molecular Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. Mohammad Javad Tahmasebi Birgani Department of Medical Physics, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. Fereshteh Negad Dehbashi Cellular and Molecular Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. Journal Article 2017 08 05 Background: The efficiency of radiotherapy for tumors can be enhanced with different radiosensitizers. Previous<br />studies have shown that electroporation (EP) can sensitize some cancer cell lines to ionizing radiation (IR). HT-29<br />is a radiation resistant colorectal cancer cell line, representative of a cancer type which is the second cause of cancer<br />mortalities in developed countries. The present study aimed to evaluate radiosensitizing effects of EP on HT-29 cells<br />in vitro exposed to 6 MV X-ray photon beams. Methods: HT-29 cells were exposed to a 6 MV X-ray photon beam<br />as the control or to a combination of electroporation and irradiation. The response of cells was evaluated by colony<br />formation assay and survival curves. Results: The survival fraction of the HT-29 cells was significantly decreased by<br />electroporation prior to radiotherapy. A single electric pulse increased colorectal HT-29 cancer cell sensitivity to megavoltage<br />radiation by a factor of 1.36. Conclusion: Our findings showed that EP before radiotherapy can significantly<br />enhance tumor cell sensitivity. This combined treatment modality should be assessed for its applicability in clinic settings<br />for employment against radioresistant cancers. However, to facilitate achieving this goal, many different tumors with<br />a broad range of radiosensitivities should be evaluated. https://journal.waocp.org/article_60721_3c8406edb75b813619e0acc54afc73d4.pdf