West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter. Asian Pacific Journal of Cancer Prevention 1513-7368 19 7 2018 07 01 Exopolysaccharide from Marine Bacillus velezensis MHM3 Induces Apoptosis of Human Breast Cancer MCF-7 Cells through a Mitochondrial Pathway 1957 1963 64825 10.22034/APJCP.2018.19.7.1957 EN Mahgoub M Ahmed Molecular Drug Evaluation Department, National Organization for Drug Control and Research, Giza, Egypt. Manal G Mahmoud Department of Microbial Biotechnology, National Research Centre, Dokki, Cairo, Egypt. Manal S Selim Department of Microbial Biotechnology, National Research Centre, Dokki, Cairo, Egypt. Mohamed E EL Awady Department of Microbial Biotechnology, National Research Centre, Dokki, Cairo, Egypt. Journal Article 2018 02 27 Objective: The production of new natural pharmaceutical agents that increase the efficiency of chemotherapy<br />without affecting the normal cells is the goal of all researchers. Therefore, the present study expects to evaluate<br />the antioxidant and anticancer studies against MCF-7 cell lines of EPS produced by novel Egyptian marine bacterial<br />strain. Methods: Marine bacterium was isolated, purified and identified by 16S rRNA gene amplification and sequence<br />analyses. MHMEPS (the produced EPS) was analyzed by Fourier Transform Infra-red (FTIR), monosugars identification<br />by HPLC, molecular weight estimation and sulfur content were determined. While, in-vitro antioxidants characters<br />was determined using various methods and anticancer studies against MCF-7 cell lines. Results: Bacillus velezensis<br />MHM3 produced 5.8 g/L of MHMEPS. The chemical analysis of MHMEPS showed 24% uronic acid and 18.19%<br />sulfate and monosugars glucuronic acid, glucose, fructose and rhamnose with molar ratio of 4.00: 2.00: 1.00: 0.13,<br />correspondingly, with an overall weight average molecular weight Mw of 1.145×104 g/mol and the number average of<br />molecular weights Mn of 5.155 ×103 g/mol. The FTIR analysis and periodate oxidation indicate the existence of<br />α-(1–4) linkage acidic polysaccharide. MHMEPS showed antioxidant scavenging activity against DPPH•, H2O2 and<br />Metal chelating activity, respectively. So, reducing power method give high activity at 500 μg/ml. MHMEPS hinder<br />the proliferation of MCF-7 cells at 5-80 μg/ml compared to the control group. Moreover, induced apoptosis was<br />associated with activation of caspase-3. Also increased cytochrome C levels significantly in a dose-dependent manner<br />compared with the control. The Caspase-3 activity was raised in MHMEPS treated MCF-7 cells compared with<br />the control (p<0.05) in a dose-dependent manner. Therefore, the result of DNA fragmentation was confirmed by DNA<br />ladder assay. We presume that MHMEPS has high potential at its low concentration, as a novel restorative agent for<br />the treatment of MCF-7 cells, with no cytotoxicity against normal cells. https://journal.waocp.org/article_64825_593bd060e2c076c1c0ec82a5086e2b7e.pdf