West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter. Asian Pacific Journal of Cancer Prevention 1513-7368 19 7 2018 07 01 Molecular Grading of Oral Squamous Cell Carcinomas Infected with EBV 1793 1796 65158 10.22034/APJCP.2018.19.7.1793 EN Theresia Indah Budhy Departement of Oral Pathology and Maxillofacial, Faculty of Dental Medicine, Universitas Airlangga, Mayjen. Prof. Dr. Moestopo 47, Surabaya, Indonesia. 0000-0002-5068-4493 Journal Article 2017 09 07 Background: Squamous Cell Carcinoma (SCC) is a type of cancer that is often found in oral cavity and areas of<br />the head and neck. Viruses are major etiological factors through production of factors that can disturb proliferation<br />and apoptosis regulators such as p53i, c-myc and bcl-2. This study aimed to determine the molecular grading of oral<br />squamous cell carcinoma (OSCCs) infected with the Epstein-Barr Virus (EBV). Methods: Twenty-seven OSCC patients<br />underwent biopsy to detect EBV infection through in situ hybridization for RNA EBV (EBER) and immunohistochemical<br />analysis of latent membrane protein-1 (LMP-1) and EBV nuclear antigen-1 (EBNA-1). To assess molecular grades, cell<br />proliferation and apoptosis regulator expression i.e. inactive p53 (p53i), c-myc and bcl-2, were immunohistochemically<br />analysed. Results: The cases were divided into two groups; infected and non-infected by EBV. Regression analysis<br />showed that only EBNA-1 expression could affect p53i expression. Based on regression equations molecular grading of<br />OSCCs infected by EBV was divided into three: Grade I (low), EBNA-1 expression was 7.60, and p53i expression<br />was 9.74-17.5; Grade II (medium), EBNA-1 expression was 7.61-19.7, and p53i 17.5-30.1; Grade III (high), EBNA-1<br />expression was 19.71, and p53i ≥ 30.1. Conclusion: In OSCC infected with EBV, only EBNA-1 expression can<br />influence p53i expression. https://journal.waocp.org/article_65158_93453e3ea136fbfcdad017657ea7f2ff.pdf