West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter. Asian Pacific Journal of Cancer Prevention 1513-7368 19 7 2018 07 01 p53 Overexpression in Oral Mucosa in Relation to Shisha Smoking in Syria and Lebanon 1879 1882 65366 10.22034/APJCP.2018.19.7.1879 EN Khaled Zaid Faculty of Dentistry, Al-Sham Private University, Damascus, Syria. 0000-0003-1034-4061 Elie Azar-Maalouf Department of Periodontology, Lebanese University, Lebanon. Charif Barakat Department of Oral Histology and Pathology, Faculty of Dentistry, Damascus University Damascus, Syria. Mansour Chantiri Department of Periodontology, Lebanese University, Lebanon. Journal Article 2018 01 10 Introduction: Shisha (waterpipe) smoking is becoming a very prevalent form of tobacco consumption in the Middle<br />east and use is growing over the world. Smoking-related malignancies have a high genome-wide burden of mutations,<br />including examples in the gene encoding p53. Aims: To investigate alterations in p53 immunohistochemical expression<br />in the normal, pre-malignant, malignant oral mucosa in relation to Shisha smoking habits. Materials and Methods:<br />A total of 105 paraffin embedded tissue sections of OSCCs (52 smokers,53 non-smokers), 96 of premalignant lesions<br />(48 smokers,48 non-smokers) and 60 normal oral mucosa. Some 30 patients with a history of Shisha smoking daily<br />for more than 5 years were also investigated for mutant expression of p53. Tissue samples were considered positive<br />for p53 staining when any positive cells of epithelial origin could be detected. Results: The majority (74.3%) of oral<br />squamous cell carcinomas showed positive staining for p53 expression (83.1% and 65.5% with Shisha smokers and<br />non-smokers, respectively). In the 96 premalignant lesions, about 23% from non-smokers and 41.7% from smokers<br />showed p53 positivity. In normal epithelium, P53 positive cells were noted in 6.6% of non-smokers and 16.6% of<br />smokers. Positive correlations with Shisha smoking were evident for the following groups: WDOSCC, MDOSCC,<br />mild dysplasia G1, moderate dysplasia G2 and in normal mucosa using Student’s t- test, P valueThese results strongly suggest that p53 mutations are associated with Shisha smoking in OSCC, pre-malignant lesions<br />and normal mucosa of the oral cavity. https://journal.waocp.org/article_65366_aff5b4bca15838e56a6301d144992ee1.pdf