West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter. Asian Pacific Journal of Cancer Prevention 1513-7368 19 8 2018 08 01 Derivative of Stevioside; CPUK02; Restores ESR1 Gene Methylation in MDA-MB 231 2117 2123 66112 10.22034/APJCP.2018.19.8.2117 EN Saeed Khazayel Department of Biochemistry, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran. Pooneh Mokarram Department of Biochemistry, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran. Gasteroenterohepatology Research Center, Nemazee Hospital, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran. Zeinab Mohammadi Department of Biochemistry, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran. Fatemeh Ramezani Department of Biochemistry, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran. Zhang Dayong Vice Director of Lianyungang Eco. & Tech. Development Zone Management Committee and Drug Research Institute, China Pharmaceutical University, China. Journal Article 2017 07 31 Background: CPUK02 (15-Oxosteviol benzyl ester) is a new ent-kaurenoid derivative of stevioside and exhibits<br />strong anti-cancer activity. Nowadays, the pattern of epigenetic in cancer has been topic of many studies and DNA<br />methylation targeting represents a relevant strategy for cancer treatment. Since, no study conducted to this mechanism, we<br />attempt to evaluate whether CPUK02 induce its anti-cancer effects via alteration the level of mRNA DNMT3B, DNMT3A<br />expression and ESR1 methylation pattern in breast cancer cells line. Methods: MCF-7 (ER +) and MDA-MB231 (ER-)<br />cell lines were treated for 24, 48 hours with 1 μM CPUK02 and 5-AZA-CdR (DNA methyltransferase inhibitor).<br />Quantitative expression of DNMT3B and DNMT3A genes and ESR1 promoter methylation was assessed by Real-Time<br />PCR and MS-PCR, respectively. Results: CPUK02 restored ESR1 promoter unmethylated allele in MDA-MB 231<br />cells. Also treatment with CPUK02 decreased the expression of both DNMT3A and DNMT3B genes like 5-AZA.<br />The expression of DNMT genes were diminished by half compared with control cells. Conclusions: These results showed<br />that CPUK02 has an anticancer effect on MDA-MB 231 cells which this effect can be done through several pathways. https://journal.waocp.org/article_66112_cc0f0c9916ea739318c09edd6953a70c.pdf