West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter. Asian Pacific Journal of Cancer Prevention 1513-7368 19 8 2018 08 01 Immune Response to Human Papillomavirus One Year after Prophylactic Vaccination with AS04-Adjuvanted HPV-16/18 Vaccine: HPV-Specific IgG and IgA Antibodies in the Circulation and the Cervix 2313 2317 66161 10.22034/APJCP.2018.19.8.2313 EN Ana Paula Ferreira Costa Postgraduate Program in Health Sciences, Federal University of Rio Grande do Norte, Natal, Brazil. Ana Katherine Goncalves Postgraduate Program in Health Sciences, Federal University of Rio Grande do Norte, Natal, Brazil. Department of Gynecology, and Obstetrics, Federal University of Rio Grande do Norte, Natal, Brazil. Paula Renata Lima Machado Department of Clinical Analysis and Toxicology, Federal University of Rio Grande do Norte, Natal, Brazil. Luanda B. F. C. De Souza Department of Clinical Analysis and Toxicology, Federal University of Rio Grande do Norte, Natal, Brazil. Ayane Sarmento Department of Clinical Analysis and Toxicology, Federal University of Rio Grande do Norte, Natal, Brazil. 0000-0001-9131-1952 Ricardo Ney Oliveira Cobucci Department of Gynecology and Obstetrics, University Potiguar, UnP Natal, Brazil. Paulo César Giraldo Department of Gynecology, and Obstetrics, State University of Campinas, Campinas, Brazil. Steven S Witkin Department of Obstetrics and Gynecology Weill Cornell Medical College, New York, NY, USA. Journal Article 2018 03 31 Objective: This study was designed to describe the course of IgG/IgA responses in cervical secretions and in serum<br />one year after the first dose of intramuscular administration of the HPV16/18 AS04-adjuvant vaccine. Methods: Blood<br />and cervical mucus samples were collected for immunologic assays, 7 months after the first doses and 1 year following<br />the last boost vaccination (month 7) by enzyme linked immunosorbent assay (ELISA). The detection of IgG and IgA<br />anti-HPV/VLP was developed for this purpose. Result: A total of 100% of serum samples were IgG antibody positive<br />at a titer of 1:100 at both time periods and decreased according to the serum dilution. For serum IgA antibody, 95%<br />were positive one month after vaccination and 79% were positive 1 year later. Similar results were observed with<br />the cervical samples positive for both IgG and IgA antibodies at one month and decreasing after 1 year to 33% and<br />29%. The median absorbance in serum and the cervix for IgG and IgA anti-HPV-VLP antibodies was significantly<br />higher at one month after vaccination when compared to 1 year post-vaccination (P<0.0001). Conclusion: Immune<br />responses were significant one year after immunization, however it decreased in cervical and serum samples when<br />compared to levels observed one month after the last dose. This suggests that a vaccine booster may be necessary to<br />increase antibody titers. https://journal.waocp.org/article_66161_0ec80904340126b5e1be81d61853cd4c.pdf