West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter. Asian Pacific Journal of Cancer Prevention 1513-7368 19 9 2018 09 01 Involvement of microRNA-423 Gene Variability in Breast Cancer Progression in Saudi Arabia 2581 2589 66655 10.22034/APJCP.2018.19.9.2581 EN R Mir Department of Medical Lab Technology, Prince Fahd Bin Sultan Research Chair, Faculty of Applied Medical Sciences,University of Tabuk, Tabuk, Kingdom of Saudi Arabia. I A Al Balawi Department of Surgical Oncology, Faculty of Medicine University of Tabuk, Tabuk, Kingdom of Saudi Arabia. 0000-0002-2952-9501 F M Abu Duhier Department of Medical Lab Technology, Prince Fahd Bin Sultan Research Chair, Faculty of Applied Medical Sciences,University of Tabuk, Tabuk, Kingdom of Saudi Arabia. Journal Article 2018 04 04 Aim: microRNA-423 is an oncogenic factor which is frequently upregulated in cancer. However, associations with<br />breast cancer risk remain inconsistent. Therefore, we investigated the prevalence of microRNA-423 rs6505162C>T<br />gene variation with breast cancer susceptibility in Saudi women. Methodology: This study was conducted on 100<br />breast cancer patients and 124 matched healthy individuals. Genotyping of the microRNA-423 rs6505162C/T gene<br />variation was performed by using the amplification refractory mutation system PCR method (ARMS-PCR). Results:<br />A significant difference was observed in the genotype distribution between the breast cancer cases and controls<br />(p=0.0001), the frequencies of the genotypes CC,CT and TT being 25%, 52% and 23% in patients and 65%,20% and<br />15% respectively, in controls. The microRNA-423 C>T variant was associated with an increased risk of breast cancer<br />in codominant models for (OR = 6.73, 95 % CI, 3.50-12.97; RR 2.35(1.67-3.30, p=0.0001) the microRNA-423TT<br />genotype and (OR = 4.14, 95 % CI, 1.93-8.87; p=0.0003) microRNA-423CT (OR= 6.73, 95% CI, 3.50-12.97; p=0.0001)<br />and also with the dominant model (OR 5.6(3.14-1.01), p=0.0001) CT+TT vs CC) with a non-significant association for<br />the recessive model (OR=1.75, 95%CI=0.08-3.44, P=0.139, TT vs CC+CT). The T allele significantly increased the<br />risk of breast cancer (OR =2.63, 95 % CI, 1.77-3.91; p=0.001) compared to the C allele. Some 6.73 ,4.14 and 2.63 fold<br />increased risk of developing breast cancer was associated with TT and CT genotypes and the T allele of microRNA-423<br />in the northwestern region of Saudi Arabia. Conclusion: Our findings indicate that the microRNA-423 TT genotype<br />and the T allele are associated with an increased susceptibility, metastasis and advanced stage of breast cancer in Saudi<br />Arabian patients. Further studies with larger sample sizes are necessary to confirm our findings. https://journal.waocp.org/article_66655_c9bba32c587d227301810257e7b17ca2.pdf