West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter. Asian Pacific Journal of Cancer Prevention 1513-7368 19 11 2018 11 01 Tylophorine Abrogates G2/M Arrest Induced by Doxorubicine and Promotes Increased Apoptosis in T47D Breast Cancer Cells 3065 3069 76627 10.31557/APJCP.2018.19.11.3065 EN Nofran Putra Pratama Postgraduate Program of Pharmaceutical Sciences of Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, Indonesia. Septi Wulandari Postgraduate Program of Pharmaceutical Sciences of Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, Indonesia. Agung Endro Nugroho Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, Indonesia. 0000-0001-7840-8493 Nanang Fakhrudin Department of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, Indonesia. 0000-0001-7954-9503 Puji Astuti Department of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, Indonesia. - Sudarsono Department of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, Indonesia. Journal Article 2017 10 30 Background: The effects of tylophorine, a natural alkaloid found in Tylophora indica, administered as a single<br />compound or in combination with doxorubicin on cell cycling and apoptosis were assessed in T47D breast cancer cells,<br />selected as a model system for breast cancer. Methods: Cell cycle distribution and apoptosis were examined by flow<br />cytometry. Caspase 3 and 9 expression was determined by immunocytochemistry.Result: We found that tylophorine did<br />not significantly influence the cell cycle distribution of T47D cells. However, the alkaloid did prevent accumulation of<br />cells in the G2/M phase. In addition, tylophorine increased the number of apoptotic cells. Expression of proapoptotic<br />proteins (caspases 3 and 9) was up-regulated upon administration of tyloporine alone or in combination with doxorubicin.<br />Conclusions: Tylophorine alone or in combination with doxorubicin induced apoptosis in T47D breast cancer cells<br />through modulation of the cell cycle and affecting the expression of caspases 3 and 9. https://journal.waocp.org/article_76627_f8efef4b7b73f865d2be777d139eab9e.pdf