West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter. Asian Pacific Journal of Cancer Prevention 1513-7368 20 3 2019 03 01 Evaluation of Cisplatin Efficacy on HepG2 and E. coli Cells under Acidic Conditions 723 726 81620 10.31557/APJCP.2019.20.3.723 EN Faezeh Babaei Anatomical Sciences Research Center, Kashan University of Medical Sciences, Kashan, Iran. Hasan Ebrahimi Shahmabadi Department of Microbiology, Faculty of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran. Mohammad Reza Rajabi Faculty of Medicine, Shahed University of Medical Sciences, Tehran, Iran. Hamed Haddad Kashani Anatomical Sciences Research Center, Kashan University of Medical Sciences, Kashan, Iran. Fatemeh Izadpanah Food and Drug Laboratory Research Center and Food and Drug Reference Control Laboratories Center, Food and Drug Administration of Iran, MOH and ME, Tehran, Iran. Journal Article 2018 02 03 Background: Cisplatin (Cispt) is a common anticancer drug for the treatment of several malignancies, including<br />hepatocarcinoma. However, this drug suffers from instability in aqueous solutions. The study aimed to evaluate cisplatin<br />efficacy on HepG2 and E. coli cells under an acidic condition. Methods: Acidic Cispt was prepared via incubation in<br />acidic condition (pH=2) for a month duration. The chemical structure of the acidic Cispt was evaluated by using Fourier<br />Transform Infrared Spectroscopy (FTIR) method. The cytotoxicity of the standard and acidic Cispt were then determined<br />by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and minimum inhibitory concentration (MIC)<br />assays on HepG2 and E. coli cells, respectively. Results: After preparing of acidic Cispt, its chemical structure was<br />determined by FTIR method. In addition, cytotoxicity effects of Cispt in the standard and acidic forms were calculated<br />58 ± 2.9 and 65 ± 3.25 μM, respectively. MIC results also confirmed the results of MTT assay. MIC results for the<br />standard and acidic Cispt were estimated 9.5 ± 0.47 and 9.8 ± 0.49 μM, respectively. Conclusion: Preparing Cispt in<br />acidic condition not only did not degrade the drug, but also kept the potency of the drug approximately equal to parent<br />drug. Regarding the instability issues of Cispt, keeping Cispt in acidic condition could be a promising approach to<br />preserve its efficacy. https://journal.waocp.org/article_81620_e525d17e475a1f8d0a0a9686e674cc4f.pdf