West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter. Asian Pacific Journal of Cancer Prevention 1513-7368 20 8 2019 08 01 Gene Combination of CD44 rs187116, CD133 rs2240688, NF-κB1 rs28362491 and GSTM1 Deletion as a Potential Biomarker in Risk Prediction of Breast Cancer in Lower Northern Thailand 2493 2502 88696 10.31557/APJCP.2019.20.8.2493 EN Kamonpat Sapcharoen Department of Biochemistry, Faculty of Medical Science, Naresuan University, Phitsanulok, Thailand. Phanchana Sanguansermsri Department of Biochemistry, Faculty of Medical Science, Naresuan University, Phitsanulok, Thailand. Sukkid Yasothornsrikul Department of Biochemistry, Faculty of Medical Science, Naresuan University, Phitsanulok, Thailand. Kanha Muisuk Department of Forensic Medicine, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand. Metawee Srikummool Department of Biochemistry, Faculty of Medical Science, Naresuan University, Phitsanulok, Thailand. Center of Excellence in Medical Biotechnology, Faculty of Medical Science, Naresuan University, Phitsanulok, Thailand. Journal Article 2019 06 15 Background: Biomarkers play an important role in oncology, including risk assessment, treatment prediction, and<br />monitoring the progression of disease. In breast cancer, many genes are used as biomarkers. Since, several SNP variations<br />of hallmark – related genes have been reported to be of value in risk prediction in various cancers and populations, some<br />genetic polymorphism loci were combined and reported as biomarkers for use in the risk assessment of breast cancer<br />in Thai people. Methods: Twelve cancer gene hallmarks (15 polymorphic loci) were selected and genotyped in 184<br />breast cancer patients and 176 healthy individuals in Phitsanulok, Thailand. Results: AA genotype of CD44 rs187116<br />(c.67+4883G>A), the C allele of CD133 rs2240688 (c.*667A>C), the *2 allele (4 bp deletion) of NF-κB1 rs28362491<br />and the homozygous null allele genotype of GSTM1 were significantly associated with an increased risk of breast cancer<br />(p<0.05). A combination of these 4 significant loci showed that AA-AA-*1*1-homozygous null allele genotype has the<br />greatest correlation with increased risk of breast cancer (OR = 21.00; 95% CI: 1.77 to 248.11; p = 0.015), followed by<br />GA-AA-*2*2- homozygous null allele genotype (p = 0.037) and GG-AC-*1*2- homozygous null allele genotype (p<br />= 0.028). Conclusion: These findings suggest that the polymorphisms of CD44 rs187116 (c.67+4883G>A), CD133<br />rs2240688 (c.*667A>C), NF-κB1 rs28362491 and GSTM1 homozygous null allele genotype might be associated with<br />an increased risk of breast cancer, and this gene combination could possibly be used as biomarkers for risk prediction,<br />which would be of benefit in planning health surveillance and cancer prevention. https://journal.waocp.org/article_88696_d174632afbf51999a9459ac2ded9536f.pdf