West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter. Asian Pacific Journal of Cancer Prevention 1513-7368 20 9 2019 09 01 PIK3CA and PTEN Genes Expressions in Breast Cancer 2841 2846 88745 10.31557/APJCP.2019.20.9.2841 EN Nabil Hadi Alowiri Department of Molecular Biology, Genetic Engineering and Biotechnology Research Institute, University of Sadat City, Sadat City, Egypt. Shaden Muawia Hanafy Department of Molecular Biology, Genetic Engineering and Biotechnology Research Institute, University of Sadat City, Sadat City, Egypt. Reham Abdel Haleem Department of Clinical Pathology, Faculty of Medicine, Alexandria University, Alexandria, Egypt. Ahmed Abdellatif Surgical Oncology Unit, Department of General Surgery, Faculty of Medicine, Alexandria University, Alexandria, Egypt. Journal Article 2019 07 22 Background: The phosphatidylinositol-3 kinase (PI3K) intracellular signaling pathway plays an important<br />role in breast cancer. The current study aimed to evaluate the expressions of two main regulators of PI3K pathway;<br />phosphatidylinositol-3- kinase catalytic subunit alpha as activator (PIK3CA), and phosphatase and tensin-homolog<br />as inhibitor (PTEN), in breast carcinoma tissue, and compare with their expressions in adjacent normal breast tissue.<br />Methods: A total of fifty female patients with breast carcinoma from surgical oncology unit of Alexandria-Main<br />University Hospital were included in this study. The Quantitative Real Time PCR was used to quantify expressions of<br />PIK3CA and PTEN. Results: PIK3CA mRNA expression was significantly increased in breast cancer tissues compared<br />to normal breast tissues (P<0.001, Z=5.700), also PTEN mRNA expression was significantly higher in breast carcinoma<br />tissue compared to normal breast tissue (P<0.001, Z=5.362). Conclusion: Increased the expressions of PIK3CA and<br />PTEN mRNA in breast cancer tissue compared to normal breast tissue. https://journal.waocp.org/article_88745_424f3bb723f6b177971f007707b3b285.pdf