West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter. Asian Pacific Journal of Cancer Prevention 1513-7368 21 11 2020 11 01 Tissue Expression Of LPHN3 in Breast Cancer: An Immunohistochemistry Method 3339 3343 89356 10.31557/APJCP.2020.21.11.3339 EN Kwuntida Uthaisar Kotepui Medical Technology Program, School of Allied Health Sciences, Walailak University, Nakhon Si Thammarat 80161, Thailand. 0000-0002-5101-8281 Manas Kotepui Medical Technology Program, School of Allied Health Sciences, Walailak University, Nakhon Si Thammarat 80161, Thailand. Duangjai Piwkham Medical Technology Program, School of Allied Health Sciences, Walailak University, Nakhon Si Thammarat 80161, Thailand. Apiram Songsri Department of Pathology, Hatyai Hospital, Songkhla 90110, Thailand. Lek Charoenkijkajorn Department of Pathology, Hatyai Hospital, Songkhla 90110, Thailand. Tidamas Kongnok Medical Technology Program, School of Allied Health Sciences, Walailak University, Nakhon Si Thammarat 80161, Thailand. Yupaporn Chanil Medical Technology Program, School of Allied Health Sciences, Walailak University, Nakhon Si Thammarat 80161, Thailand. Journal Article 2020 07 20 Objective: Breast cancer is one of the most important public health problems among women worldwide. It is a major cause of morbidity especially among women in developing countries including Thailand. The purpose of this study was to study the expression of LPHN3 protein in normal breast tissue compared to breast cancer tissue. Methods: We had studied the expression of LPHN3 in 65 breast tissues using an immunohistochemistry method. The association between LPHN3 expression and breast cancer metastasis to nearby axillary lymph nodes was also examined. Results: Among the 65 breast cancer and normal breast tissues examined, LPHN3 expression with an immunohistochemistry index (IHC index) greater than 4 was more frequently found in breast cancer tissues than in normal breast tissues (P-value = 0.001, OR (95% CI) = 7.04 (2.16-23)). Moreover, a high expression of LPHN3 (IHC index > 4) was more frequently found in breast cancer tissues with negative axillary lymph nodes than in those with positive ones (P-value = 0.038, OR (95% CI) = 0.25 (0.07-0.96)). LPHN3 protein might be a new metastasis suppressor gene in breast cancer and a marker for breast cancer metastasis prevention. Conclusions: The present study indicated that a decrease of LPHN3 protein expression in breast cancer tissue might be a marker indicating the aggressiveness of breast cancer. These results also suggested that a decrease of LPHN3 expression could be functionally involved in breast cancer progression and metastasis. https://journal.waocp.org/article_89356_c11bca4c4fa112ffe00f0d581e7f50df.pdf