West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter. Asian Pacific Journal of Cancer Prevention 1513-7368 23 1 2022 01 01 TP53 and EGFR Mutational Status in Thymoma: A Genetic Sequencing Study 109 114 89956 10.31557/APJCP.2022.23.1.109 EN Elisna Syahruddin Department of Pulmonology and Respiratory Medicine, Faculty of Medicine, Universitas Indonesia/Persahabatan National Respiratory Referral Hospital, Jakarta, Indonesia. Human Cancer Research Centre-IMERI-Faculty of Medicine Universitas Indonesia. 0000-0002-4383-2240 Jamal Zaini Department of Pulmonology and Respiratory Medicine, Faculty of Medicine, Universitas Indonesia/Persahabatan National Respiratory Referral Hospital, Jakarta, Indonesia. 0000-0002-3573-6312 Ruth Sembiring Pathology Unit, Persahabatan National Respiratory Referral Hospital, Jakarta, Indonesia. Romi Baginta Pathology Unit, Persahabatan National Respiratory Referral Hospital, Jakarta, Indonesia. Muhammad Rizqy Fadhillah Department of Pulmonology and Respiratory Medicine, Faculty of Medicine, Universitas Indonesia/Persahabatan National Respiratory Referral Hospital, Jakarta, Indonesia. 0000-0002-3388-4864 Dimas Ramadhian Noor Human Cancer Research Centre-IMERI-Faculty of Medicine Universitas Indonesia. Journal Article 2021 06 23 Background and objective: Thymoma is a rare malignant tumor that usually with an indolent presentation, which was falsely assumed to be benign previously. The tumor suppressor P53 (TP53) and EGFR gene mutate most frequently in human cancers. We tried to investigate the presence of TP53 and EGFR mutations among thymoma patients referred to an Indonesian referral respiratory hospital and to discuss its potential role in thymoma management and prognosis. Material and methods: Surgically resected tumor tissues were collected from thymoma patients and then underwent genomic analysis. PCR was performed on the extracted Paraffinized  DNA to amplify exon 6 of TP53 and exons 18, 19, and 21 of EGFR. The evaluation of mutational status was done using direct sequencing and sequence analysis of purified PCR products. Results: Among 27 collected samples, TP53 exon 6 mutation, namely  missense mutation and nonsense mutation, was observed in two samples (7.4%). EGFR exon 18 mutation, namely E709K and nonsense mutation, was found in 2 samples (7.4%). An intronic mutation in EGFR exon 19 (3.7%) and exon 21 (3.7%) was observed in one sample. Conclusion: TP53 and EGFR mutations were not most frequent, so it seems that these genes are not involved in the pathogenesis of thymoma in Indonesian patients. Nevertheless, we found two samples with a significant mutation in p53 and EGFR genes, suggesting further research on thymoma prognostification and targeted therapy. https://journal.waocp.org/article_89956_f640c86f602684fe01bd3d2034845466.pdf