West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter. Asian Pacific Journal of Cancer Prevention 1513-7368 23 4 2022 04 01 Brazilin Isolated from Caesalpina Sappan Wood Induces Intrinsic Apoptosis on A549 Cancer Cell Line by Increasing p53, caspase-9, and caspase-3 1337 1343 90072 10.31557/APJCP.2022.23.4.1337 EN Suyatmi Suyatmi Doctoral Program of Medical Sciences , Faculty of Medicine, Universitas Sebelas Maret, Surakarta, Central Java, Indonesia. Department of Histology, Faculty of Medicine, Universitas Sebelas Maret, Surakarta, Central Java, Indonesia. 0000-0002-8223-2862 Ambar Mudigdo Doctoral Program of Medical Sciences , Faculty of Medicine, Universitas Sebelas Maret, Surakarta, Central Java, Indonesia. Department of Anatomical Pathology, Faculty of Medicine, Universitas Sebelas Maret, Surakarta, Central Java, Indonesia. 0000-0002-3498-5106 Bambang Purwanto Doctoral Program of Medical Sciences , Faculty of Medicine, Universitas Sebelas Maret, Surakarta, Central Java, Indonesia. Department of Internal Medicine, Faculty of Medicine, Universitas Sebelas Maret, Surakarta, Central Java, Indonesia. Dono Indarto Doctoral Program of Medical Sciences , Faculty of Medicine, Universitas Sebelas Maret, Surakarta, Central Java, Indonesia. Department of Physiology and Biomedical Laboratory, Faculty of Medicine, Universitas Sebelas Maret, Surakarta, Central Java, Indonesia. Fikar Arsyad Hakim Department of Anatomical Pathology, Faculty of Medicine, Universitas Sebelas Maret, Surakarta, Central Java, Indonesia. Dyah Ika Krisnawati Dharma Husada Nursing Academy, Kediri 64114, East Java, Indonesia. Journal Article 2021 11 15 Objective: Lung cancer is the leading cause of death among cancer patients. The majority of lung cancer is the Non-Small Lung Carcinoma (NSLC). This study evaluated the potency of brazilin isolated from Caesalpinia sappan wood to induce apoptosis on non-small lung carcinoma cell line, A549, by examining the expression of p53, caspase-9, and caspase-3. Methods: Brazilin was isolated from Caesalpinia sappan wood following a guided assay and it was determined by using Brazilin®SIGMA as standard. The activity of brazilin on the growth of A549 cell line was analysed by MTT assay and the apoptosis was evaluated by flowcytometer following Annexin V (FITC) and PI staining. The expression of p53, caspase-9, and caspase-3 was examined by immunocytochemistry. Result: The IC50 of brazilin on A549 cell line was 43µg/mL. Cell treatment with 20 µg/mL and 40 µg/mL of brazilin significantly increased early apoptosis (p<0.001). Cell treatment with 40 µg/mL  of Brazilin significantly increased late apoptosis (p<0.001). Brazilin significantly increased the expression of p53, Caspase-9, and caspase-3 (p<0.001). Conclusion: This study showed evidence of the activity of brazilin to induce intrinsic apoptosis on a NSLC cell line A549.  https://journal.waocp.org/article_90072_36b5d2823440eb7330ce23f330c691e5.pdf