West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter. Asian Pacific Journal of Cancer Prevention 1513-7368 24 2 2023 02 01 DNA Damage on Buccal Epithelial Cells, Personal Working in the Rubber Industry Occupationally Exposed to Carbon Disulfide (CS2) 357 361 90503 10.31557/APJCP.2023.24.2.357 EN Manikantan Pappuswamy Department of Life Sciences, CHRIST (Deemed to be University), Bangalore, Karnataka, India. 0000-0002-6047-3702 Aditi Chaudhary Department of Life Sciences, CHRIST (Deemed to be University), Bangalore, Karnataka, India. Arun Meyyazhagan Department of Life Sciences, CHRIST (Deemed to be University), Bangalore, Karnataka, India. Karthick Kumar Alagamuthu Department of Biotechnology, Selvam Arts and Science College, Namakkal, Tamilnadu, India. Balamuralikrishnan Balasubramanian Department of Food Science and Biotechnology, College of Life Science, Sejong University, Seoul 05006, Republic of Korea. Vijaya Anand Arumugam Medical Genetics and Epigenetics Laboratory, Department of Human Genetics and Molecular Biology, Bharathiar University, Coimbatore 641046, Tamil Nadu, India. Joseph Kadanthottu Sebastian Department of Life Sciences, CHRIST (Deemed to be University), Bangalore, Karnataka, India. Journal Article 2022 10 01 Introduction: The most significant industrial utilization of carbon disulfide (CS2) has been in the manufacture of cellulose rayon, cellophane, and rubber industry. CS2 prompts expanded recurrence of chromosomal variations in laborers occupationally exposed to CS2. Materials and Methods: In the current study, the DNA analysis was carried out from exfoliated buccal epithelial cells from rubber industry workers exposed to CS2 and an equal number of healthy control subjects. Both the control and experimental subjects were categorized by their smoking habits such as smokers (S) and non-smokers (NS). Furthermore, experimental subjects were further separated based on their exposure period. Students t-test statistical tools were used to analyze the final results. Results: The present analysis identified a high frequency of DNA damage in rubber industry workers (16.55±0.43) than control subjects (9.8±0.21). Also, maximum number of DNA damage detected in smoking experimental group (18.27±0.02) than non-smoking experimental (15.02±0.01) and smoking control groups (10.25±0.04 ). Conclusion: Smoking habits synergistically increased the DNA damage in the rubber industry workers exposed to CS2. https://journal.waocp.org/article_90503_b9e9efc1a22577da140b9b3b44c89db4.pdf