West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter. Asian Pacific Journal of Cancer Prevention 1513-7368 24 4 2023 04 01 Down-regulation and Clinic-pathological Correlation of SIK-1 and SIK-1-LNC in Non-small Cell Lung Cancer Patients 1407 1411 90589 10.31557/APJCP.2023.24.4.1407 EN Alireza Anvarnia Tuberculosis and Lung Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. Shohada Clinical Research Development Unit, Shohada Hospital, Tabriz University of Medical Science, Tabriz, Iran. 0000-0003-4589-1129 Reza Panahizadeh Students Research Committee, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran. 0000-0002-2314-7158 Habib Zarredar Tuberculosis and Lung Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. 0000-0002-7217-7521 Milad Asadi Department of Basic Oncology, Ege University, Institute of Health Sciences, Izmir, Turkey. 0000-0001-7846-2908 Shahriyar Hashemzadeh Tuberculosis and Lung Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. 0000-0002-1162-763X Mohammad Amin Vatankhah Students Research Committee, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran. 0000-0002-8855-6614 Hamed Valizadeh Tuberculosis and Lung Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. 0000-0003-2402-654X Mortaza Raeisi Hematology and Oncology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. 0000-0002-3173-1610 Journal Article 2023 01 07 Background: Non-small-cell lung cancer (NSCLC) is currently the leading cause of mortality cancer. Introducing noninvasive approaches to diagnose NSCLC, especially at an early phase, might improve the disease’s prognosis. Long noncoding RNAs (lncRNAs), which are important regulators of the expression genes inside the cells, have been linked to a range of biological processes, such as cancer progression and metastasis, including NSCLC. The present work aims to determine the potential involvement of SIK-1-LNC and SIK-1 in NSCLC pathogenesis and the possible use of these molecules as novel biomarkers or therapeutic targets. Methods: In this work, the expression levels of SIK-1-LNC and SIK-1 in 50 pairs of NSCLC tumor and tumor marginal tissues were evaluated. So, after total RNA extraction and complementary DNA synthesis, the SIK-1-LNC and SIK-1 expression levels were evaluated by real-time PCR. In the study groups, clinical and pathological characteristics of the NSCLC patients were also examined. Results: Our findings showed that tumor samples had much lower levels of SIK-1 and SIK-1-LNC expression than tumor margin samples. SIK-1-LNC expression was correlated with SIK-1 levels in NSCLC samples. Interestingly, both stage and lymph node metastasis features of the tumor were associated significantly with SIK-1 and SIK-1-LNC expression levels. A ROC curve analysis indicated a biomarker index of 0.69 and 0.74 for SIK-1 and SIK-1-LNC, respectively. Conclusion: Collectively, our study emphasized the role of SIK-1-LNC and SIK-1 downregulation in NSCLC oncogenesis. Additionally, SIK-1 and SIK-1-LNC, particularly the latter, have shown remarkable potential to be utilized as new NSCLC biomarkers and therapeutic targets. https://journal.waocp.org/article_90589_11be490cf9d20aeeb708cb963dc8229b.pdf