West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter. Asian Pacific Journal of Cancer Prevention 1513-7368 25 6 2024 06 01 Investigation of TLR2 (-196 to -174del) and TLR9 (T-1486C) Gene Polymorphisms Association with Inflammatory Bowel Diseases 2003 2010 91167 10.31557/APJCP.2024.25.6.2003 EN Roseline Eke Emele Department of Biotechnology, Institute of Natural and Applied Sciences, University of Niğde Ömer Halisdemir, Niğde, Turkey. 0000-0001-6033-7199 Mohammed A Salim Department of Biotechnology, Institute of Natural and Applied Sciences, University of Niğde Ömer Halisdemir, Niğde, Turkey. Biotechnology- Proteomics and Molecular Biology, Africa City of Technology, Ministry of Higher Education And Scientific Research, Khartoum, Sudan. 0000-0002-5897-2289 Fikriye Polat Department of Mathematics and Sciences Education, Faculty of Education, University of Kocaeli, Kocaeli, Turkey. 0000-0002-5414-2501 Songül Budak Diler Department of Biotechnology, Faculty of Science and Letters, University of Niğde Ömer Halisdemir, Niğde, Turkey. 0000-0002-7156-583X Journal Article 2023 11 21 Background: Inflammatory bowel diseases (IBD), Crohn’s disease (CD), and ulcerative colitis (UC) are diseases that result from the combined effects of a predisposing genetic background and several environmental factors, including smoking. Some genes can influence these diseases through genetic inheritance, and their regulation is explained by gene polymorphism. However, Toll-like receptor (TLR) genes have been identified as susceptibility genes for CD and UC. Methods: A case-control study was performed on a Turkish population composed of 105 healthy controls and  79 CD, 77 UC patients genotyped by Allele-specific PCR and PCR–RFLP for TLR9 (T-1486C) and TLR 2 (-196 to -174del) gene. Genotype and allele frequencies of TLR9 (T-1486C) and TLR 2 (-196 to -174del) gene polymorphisms compared to allele frequencies in CD and UC patients. Results: No statistically significant findings were found between the CD, UC patients, and the control group in terms of both genotype distributions and allele frequencies for TLR 9 (T-1486C; rs187084) and TLR 2 (-196 to -174del; rs111200466) gene polymorphisms in a Turkish population (P > 0.05). Conclusion: No association was found between the TLR2 (rs111200466) and TLR 9 (rs187084) gene polymorphisms among IBD patients and the control groups in the Turkish population. https://journal.waocp.org/article_91167_518feccfdf7b9ef805f8af1901c86843.pdf