West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter. Asian Pacific Journal of Cancer Prevention 1513-7368 25 9 2024 09 01 Oncolytic Newcastle Disease Virus and Photodynamic Therapy as Dual Approach for Breast Cancer Treatment 3111 3118 91337 10.31557/APJCP.2024.25.9.3111 EN Shaymaa Majeed Hamzah Al-Shammari Ministry of Health, Al Diwaniya Health Bureau, Women and Children Hospital, Baghdad, Iraq. Ahmed S. K. Al-Khafaji Department of Biology, College of Science, University of Baghdad, Baghdad, Iraq. 0000-0002-5077-1036 Haider A. Hassan Department of Biotechnology, College of Science, University of Baghdad, Baghdad, Iraq. Marwa Ibrahim Salman Department of Biotechnology, College of Science, University of Baghdad, Baghdad, Iraq. Ahmed Majeed Al-Shammari Department of Experimental Therapy, Iraqi Center for Cancer and Medical Genetic Research, Mustansiriyah University, Baghdad, Iraq. 0000-0002-2699-1514 Journal Article 2024 03 23 Objective: We hypothesized that attacking cancer cells by combining various modes of action can hinder them from taking the chance to evolve resistance to treatment. Incorporation of photodynamic therapy (PDT) with oncolytic virotherapy might be a promising dual approach to cancer treatment. Methods: NDV AMHA1 strain as virotherapy in integration with aminolaevulinic acid (ALA) using low power He-Ne laser as PDT in the existing work was examined against breast cancer cells derived from Iraqi cancer patients named (AMJ13). This combination was evaluated using Chou–Talalay analysis. Results: The results showed an increased killing rate when using both 0.01 and 0.1 Multiplicity of infection (MOI) of the virus when combined with a dose of 6172.8 photons/gm (ph/gm) of PDT focused on cancer cells. Conclusion: integration of the attenuated NDV–AMHA1 strain with photodynamic therapy has a synergistic killing effect on breast cancer cells in vitro, suggesting that this strategy could have clinical application to overcome breast cancer. https://journal.waocp.org/article_91337_888be9cb4eacfff8e04b4c25c3b17260.pdf