West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter. Asian Pacific Journal of Cancer Prevention 1513-7368 26 4 2025 04 01 Anticancer Efficacy of Citrullus Colocynthis Oil as a Promising Agent in Suppressing Human Lung Adenocarcinoma A549 Cells 1243 1251 91605 10.31557/APJCP.2025.26.4.1243 EN Ahmed Naeem Al-Khammas Department of Biology, College of Education for Pure Science, University of Basrah, Basrah, Iraq. 0009-0006-3216-7915 Ali Abd Allateef Al-Ali Department of Biology, College of Education for Pure Science, University of Basrah, Basrah, Iraq. Journal Article 2024 08 15 Objective: Lung adenocarcinoma is the most common respiratory system tumor worldwide. This type of tumor facing challenges such as multidrug resistance and low selectivity.  The need for alternative therapeutic strategies motivates researchers to discover new approaches for tumor treatments. Previous studies indicate that phytochemicals may inhibit cancer development and progression. Methods: Citrullus colocynthis oil extraction was used as phytochemical therapy to inhibit cell proliferation, invasion, and enhanced cell death. The extracted oil was analyzed using GC-mass, and cell viability was assessed by MTT, apoptotic, and wound healing assay. In this study, human lung adenocarcinoma A549 cancer cell lines and human breast epithelial HBL-100 normal cell lines were used to test the efficiency of Citrullus colocynthis oil extraction on inhibiting lung cancer. Result: The results demonstrated a significant cytotoxic effect of Citrullus Colocynthis oil extraction against cancer cells, with no observed effects on normal cells. Conclusion: Phytotherapy may suppress human lung adenocarcinoma A549 cancer cell lines and offer a new strategy for future cancer therapy. https://journal.waocp.org/article_91605_6d03256c43aae8726027cb0789710070.pdf