West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736822820210801SARS-COV-2 IgG and IgM Antibodies in Cancer Patients231123118972110.31557/APJCP.2021.22.8.2311ENRujittikaMungmunpuntipantipPrivate Academic Consultant, Bangkok Thailand.VirojWiwanitkitHonorary Professor, Dr DY Patil University, Pune, India.Journal Article20210722https://journal.waocp.org/article_89721_307614b4110c37d791dc74ee102add48.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736822820210801Epithelial Dysplasia at Excision Margins of Oral Squamous Cell Carcinoma: A Review on Relationship to Clinicopathological Parameters and Prognosis231323218970910.31557/APJCP.2021.22.8.2313ENNimna HSenarathDepartment of Community Dentistry, Faculty of Dental sciences, University of Peradeniya, Sri Lanka.0000-0003-0662-6285Primali RJayasooriyaDepartment of Oral
Pathology, Faculty of Dental Sciences, University of Peradeniya, Sri Lanka.0000-0002-1991-0675Bogahawatte S.M.SSiriwardenaDepartment of Oral Pathology, Faculty of Dental Sciences, University of Peradeniya, Sri Lanka.0000-0002-5178-5026Wanninayake MTilakaratneDepartment of Oral Pathology, Faculty of Dental Sciences, University of Peradeniya, Sri Lanka.Department of Oral and Maxillofacial Clinical Sciences, Faculty of Dentistry, University of Malaya, Malaysia.0000-0002-7061-7378Journal Article20210223Background: Epithelial dysplasia (ED) at oral cancer excision margins is a frequent finding. Dysplastic epithelium at excision margins may not be similar to dysplasia in Oral potentially malignant disorders (OPMD) as malignant transformation has already taken place. Therefore, management of ED at excision margins should be different to that of OPMD. ED creates a dilemma in relation to further management of cancer patients, since there are no accepted guidelines. Therefore, the objective of this review is to analyze existing literature and to arrive at evidence based recommendations for the management of ED at excision margins. Methods: A comprehensive string was run on PubMed, Medscape and Medline. The final outcome included 113 studies. Finally, the most relevant 10 articles were critically assessed for inclusion and exclusion criteria against various parameters. Results and Conclusions: Severe and Moderate ED need re-excision in order to improve prognosis. There is not enough sound evidence for the management of Mild ED at excision margins of oral squamous cell carcinoma. Guidelines for the management of ED at excision margins should be formulated after comprehensive multi center studies using lager cohorts of patients.<br /> https://journal.waocp.org/article_89709_b600a049c2bc9a8871a495bc48943bcc.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736822820210801Association of PON1, LEP and LEPR Polymorphisms with Susceptibility to Breast Cancer: A Meta-Analysis232323348973810.31557/APJCP.2021.22.8.2323ENSoheilaSayadDepartment of Surgery, Iran University of Medical Sciences, Tehran, Iran.Seyed AlirezaDastgheibCancer Institute, Imam Khomeini Hospital, Tehran University of Medical Sciences, Tehran, Iran.MerajFarbodDepartment of Medical Genetics, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.FatemehAsadianDepartment of Medical Laboratory Sciences, School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran.MojganKarimi-ZarchiDepartment of Obstetrics and Gynecology, Iran University of Medical Sciences, Tehran, Iran.Endometriosis Research Center, Iran University of Medical Sciences, Tehran, Iran.SeyedaliSalariDepartment of Biology, Science and Arts University, Yazd, Iran.Seyed HosseinShakerDepartment of Emergency Medicine, Iran University of Medical Sciences, Tehran, Iran.JalalSadeghizadeh-YazdiDepartment of Food Science and Technology, School of Public Health, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.HosseinNeamatzadehDepartment of Medical Genetics, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.Mother and Newborn Health Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.Journal Article20210524Objective: Breast cancer is the most common cancer in American women, except for skin cancers. In this meta-analysis, the associations of polymorphisms within paraoxonase 1 (PON1), leptin (LEP) and leptin receptor (LEPR) genes with susceptibility to breast cancer were comprehensively evaluated. Methods: A universal search in PubMed, Scopus, CNKI, SID, Web of Knowledge and Google Scholar was performed to identify relevant studies up to 01 May, 2021. The strength of the associations was estimated by Odds ratios (ORs) with 95% confidence intervals (95% CIs). Results: A total of 39 case-control studies including 7 studies with 2005 cases and 2748 controls were on PON1 rs662, 6 studies with 2,031 cases and 1,973 controls on PON1 rs854560, 12 studies with 3,444 cases and 3,583 controls on LEP rs7799039, and 14 studies with 5,330 cases and 6,188 controls on LEPR rs1137101 were selected. Pooled data showed that PON1 rs662 and rs854560 polymorphisms were associated with risk of breast cancer in overall population, but not LEP rs7799039 and LEPR rs1137101. Conclusions: Our pooled data revealed that the PON1 rs662 and rs854560 polymorphisms were significantly associated with an increased risk of breast cancer in the overall population. However, LEP rs7799039 and LEPR rs1137101 polymorphisms were not associated.https://journal.waocp.org/article_89738_e8bf88cccf8e09e0708d991598420ec8.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736822820210801Evaluation of the Effect of Orlistatorlistat on Expression of OCT4, Nanog, SOX2, and KLF4 Genes in Colorectal Cancer SW40 Cell Line233523418970110.31557/APJCP.2021.22.8.2335ENMojganNoroozi KarimabadMolecular Medicine Research Center, Research Institute of Basic Medical Sciences, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.FarzadRoostaeiDepartment of Clinical Biochemistry, Faculty of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.MehdiMahmoodiDepartment of Clinical Biochemistry, Afzalipoor Faculty of Medicine, Kerman University of Medical Sciences, Kerman, Iran.0000-0002-8463-8364Mohammad RezaHajizadehMolecular Medicine Research Center, Research Institute of Basic Medical Sciences, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.Department of Clinical Biochemistry, Faculty of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.Journal Article20190615Background and Objective: Orlistat drug is one of the most criticalanti-obesity drugs that widely used around the world. The aim of this study was evaluation the effect of orlistat on the expression of OCT4, Nanog, SOX2, and KLF4 genes in the colorectal cancer SW40 cell line. Materials and Methods: SW40 cell line was cultured in DMEM medium contained orlistat for 24h, and cell viability was assessed by MTT assay. The fold changes of expression of OCT4, NANOG, KLF4, and SOX2 at mRNA level against β-actin were determined by real-timePCR. Two-sample t-test and one-way ANOVA were used to compare the mean of expression of different genes in different groups and different concentrations; a significant level of 0.05 was considered in all tests. Results: Our results showed a significant difference in cell viability, when different doses of Orlistat were used for 24 hour. concentrations of 25 and 100 μM reduce significantly the expression of OCT4 (p <0.05) and SOX2 (p <0.05) in the treated group in comparison to control (p <0.05). Also, the mRNA expression of KLF4 and Nanog was reduced significantly after treatment of SW40 cell lines was performed with 100 μM doses of Orlistat (p <0.05). Conclusion: It appears that after further studies in animal and human phases, orlistat can be used for the treatment of Colorectal Cancer.<br /> https://journal.waocp.org/article_89701_4618c8db6cc9c70df4b249fbbb324142.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736822820210801Quality of Actions to Control Cervical Cancer in Bahia, Brazil234323498971910.31557/APJCP.2021.22.8.2343ENEduarda Ferreira DosAnjosMultidisciplinary Health Institute, Federal University of Bahia, Vitória da Conquista, Bahia, Brazil.0000-0003-4322-236XPoliana CardosoMartinsMultidisciplinary Health Institute, Federal University of Bahia, Vitória da Conquista, Bahia, Brazil.0000-0002-6698-0289Nília Maria Brito De LimaPradoMultidisciplinary Health Institute, Federal University of Bahia, Vitória da Conquista, Bahia, Brazil.0000-0001-8243-5662Vanessa MoraesBezerraMultidisciplinary Health Institute, Federal University of Bahia, Vitória da Conquista, Bahia, Brazil.0000-0001-5333-2875Patty Fidelis DeAlmeidaFluminense Federal
University, Niterói, Rio de Janeiro, Brazil.0000-0003-1676-3574Adriano Maia DosSantosMultidisciplinary Health Institute, Federal University of Bahia, Vitória da Conquista, Bahia, Brazil.0000-0001-9718-1562Journal Article20200825Objective: To assess the quality of the actions to control cervical cancer (CC) and its correlates. Methods: This is a cross-sectional study conducted from January to March 2019 in 19 municipalities in Bahia, Brazil, with a sample of 241 doctors and nurses from primary health care (PHC). Three dependent variables were chosen- “Performance of educational, promotion, prevention, and monitoring actions” (D1); “Access to diagnostic tests” (D2); “Non-occurrence of high grade cervical squamous intraepithelial lesions (HSIL)” (D3). Poisson regression with robust variance was used, adopting hierarchical input variables to estimate the prevalence ratios and confidence intervals of 95%. Results: The following prevalence rates were found: D1 39.8% (95% CI: 33.8-46.2); D2 73.9% (95% CI: 67.9-79.1); and D3 46.4% (95% CI: 39.9-53.0). These dimensions remained associated with the dependent variables: D1- having professional training courses on the topic; consideration to ensure that collection takes place appropriately by a professional; and women having access to medical transport; D2- nurses treating low-grade lesions; D3- recording the Papanicolaou in electronic medical records; D1 and D2- professionals joining the service through public tender; D1 and D3- working in the PHC (≥ 2 years); D2 and D3- recording Papanicolaou in physical records; and performance of Papanicolaou by residents. Conclusion: Better trained professionals and professionals working in stable work arrangements are associated with comprehensive actions to control CC. Such strategies indicate that investments in work management result in a more organized PHC and more solution-centered work processes. Therefore, working in the PHC for a longer time and nurses performing more clinical actions (collection and treatment) are favored by such organizational actions. Investments in diagnostic support contribute to perceptions of more comprehensive actions to control CC. <br /> https://journal.waocp.org/article_89719_3ce00a2545e9d040eb4ead03c2c8d99e.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736822820210801Prevalence and Social Contextual Factors of Smokeless Tobacco Use: Insights from Schools of Delhi, India235123558970210.31557/APJCP.2021.22.8.2351ENAkash DeepSharmaDepartment of Community Medicine, Maulana Azad Medical College, Delhi, India.SuneelaGargDepartment of Community Medicine, Maulana Azad Medical College, Delhi, India.0000-0002-2196-1607Meghachandra MSinghDepartment of Community Medicine, Maulana Azad Medical College, Delhi, India.Chetana PrakashDeshmukhDepartment of Community Medicine, Maulana Azad Medical College, Delhi, India.https://orcid.org/00PragyaSharmaDepartment of Community Medicine, Maulana Azad Medical College, Delhi, India.Amod LBorleDepartment of Community Medicine, Maulana Azad Medical College, Delhi, India.Journal Article20201110Background: It was observed that adult users start tobacco use in childhood or adolescence. The influence of digital and print media, social acceptance among peers, and in order to mimic role models from films attracts youth towards tobacco. Hence this study was conducted to assess the prevalence of smokeless tobacco (SLT) use among school-going adolescents with the assessment of the influencing factors such as exposure at school, home, and public places along with the role of various media in SLT use by adolescents. Methods: Cross sectional study was conducted with 860 students of class 9th -12th enrolled in schools. Purposive sampling of three schools was done from the study area to reach desired sample size. All the students of class 9th - 12th within selected schools were given chance to participate in the study. Data collection was done using pretested modified Global Youth Tobacco Survey (GYTS) questionnaire. Results: Consumption of smokeless tobacco (SLT) was observed in 79 (11%) of 714 subjects. Significantly higher proportion (18.4%) of study subjects consumed SLT who were exposed to tobacco at school premises and 19.1% of study subjects who were exposed to teacher using SLT in schools compared to non-exposed group. (p=0.016). It was observed that 8.1% of subjects without any exposure to tobacco at home and 9.8% of subjects without exposure to tobacco at outdoor public space consumed SLT. Consumption of SLT use was highest (16.7%) among subjects exposed to tobacco advertisement or promotion through social media (p=0.04). Conclusion: High prevalence of SLT was detected among adolescents in school. Factors such as exposure to tobacco at home, public places, school and school teacher using SLT, exposure of tobacco advertisement and promotion via different modes of media was found to be significantly associated with the use of SLT in the adolescents of school.<br /> https://journal.waocp.org/article_89702_f04cde2a568839a485694672d8ea1799.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736822820210801The Effectiveness of Health Education Using Audiovisual on the Santri Smokers’ Motivation to Stop Smoking235723618972010.31557/APJCP.2021.22.8.2357ENIsmailIsmailDepartment of Nursing, Polytechnic of Health-Ministry of Aceh, Aceh, Indonesia.RapitosSidiqDepartment of Nursing, Polytechnic of Health-Ministry of Padang, Padang, Indonesia.BustamiBustamiDepartment of Nursing, Polytechnic of Health-Ministry of Aceh, Aceh, Indonesia.Journal Article20201122Background and objective: Low motivation to quit smoking affects individual smoking behavior. Health education using audiovisual media can increase smokers’ motivation to quit smoking. The objective of this study was to determine the effectiveness of health education using audiovisuals on the Santri smokers’ motivation to stop smoking. Methods: This quasi-experimental study was carried out using a pretest-posttest design. The sample in this study included Santri smokers studying at the Traditional Islamic Boarding School in Aceh Besar . This study consisted of 4 groups. Three groups were given intervention (audiovisual health education with different themes) and one group received just health education. Groups were compered in terms of difference in the mean smokers’ motivation to quit smoking. The data analysis was done by running paired t-test and one-way ANOVA. Results: The results of statistical tests showed that there was a difference in the mean motivation to quit smoking before and after the intervention in each group (mean ± SD for group 1 to 4 was 11.52 ± 4.76, 15.39 ± 6.06, 22.57 ± 6.23, and 9.84 ± 6.42, respectively). The highest increase in the mean motivation to quit smoking was allocated to group 3 who received audiovisual health education with the theme of risk of developing cancer due to smoking. Conclusion: Health education using audiovisuals could increase the motivation of students to quit smoking, especially interventions on the risk of developing cancer due to smoking. Therefore, health workers are suggested to use audiovisuals to implement various intervention in order to change smoking behavior in students.https://journal.waocp.org/article_89720_0a9869c3f16c78fa994575f1519117f4.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736822820210801Necrosis Factor-α (TNF-α) and the Presence of Macrophage M2 and T Regulatory Cells in Nasopharyngeal Carcinoma236323708970310.31557/APJCP.2021.22.8.2363ENIffahMardhiyahFaculty of Dentistry, Universitas Gadjah Mada, Yogyakarta, Indonesia.Yustina NukeArdiyanDepartment of Histology, Faculty of Medicine, Duta Wacana Christian University, Yogyakarta.Siti HamidatulAliyahFaculty of Biology, Universitas Gadjah Mada, Yogyakarta, Indonesia.Pharmacy Program, Sekolah Tinggi Ilmu Kesehatan Harapan Ibu, Jambi, Indonesia.Enda CindylosaSitepuDepartment of Histology and Cell Biology, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Yogyakarta, Indonesia.CameliaHerdiniDepartment of Otorhinolaryngology Head and Neck Surgery, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Yogyakarta, Indonesia.Ery KusDwianingsihDepartment of Pathological Anatomy, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Yogyakarta, Indonesia.https://orcid.org/00FatinAsfarinaMolecular Biology Laboratory (Integrated Research Laboratory), Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Yogyakarta, Indonesia.SumartiningsihSumartiningsihMolecular Biology Laboratory (Integrated Research Laboratory), Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Yogyakarta, Indonesia.JajahFachirohDepartment of Histology and Cell Biology, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Yogyakarta, Indonesia.0000-0002-2650-1941Dewi KartikawatiParamitaDepartment of Histology and Cell Biology, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Yogyakarta, Indonesia.Molecular Biology Laboratory (Integrated Research Laboratory), Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Yogyakarta, Indonesia.1000-0001-6759-8658Journal Article20201127Objective: To investigate the correlation between TLR3 and pro-inflammatory cytokines (TNFα, IL6) expression with the distribution of macrophage M2 and Treg on Epstein Barr virus-encoded RNAs (EBER+) nasopharyngeal carcinoma (NPC) tissues. Methods: A total of 23 FFPE NPC tissue samples were obtained from patients in Dr. Sardjito General Hospital, Yogyakarta, Indonesia in 2008–2010, which expressed EBER was collected. The expressions of TLR3, TNFα, and IL6 were examined using immunofluorescence assay. The distribution of macrophage M2 and Treg were examined by immunohistochemistry with anti-CD163 and -FOXP3 antibodies, respectively. The quantification of fluorescence intensity was analyzed by the RGB space method using ImageJ software. The M2 interpretation was done by the eyeballing method and the M2 scores were divided into 0 (negative), 1 (scant), 2 (focal), 3 (abundant). The average number of Treg FOXP3+ cells in five high power fields was counted. The relationship between variables were tested by the Spearman correlation test, and the coefficient correlation was used to see the correlation between variables. Results: All EBER+ NPC specimens showed TLR3 expression intracellularly. The expression of TNFα could be observed in the cell membranes and secreted extracellularly, while IL6 was secreted to the extracellular area. The expression of TNFα was two times higher than IL6. Most specimens showed low M2 score (56.52%) and high Treg (52.17%). A positive correlation was found between TLR3 and IL6 (12.9%). TNFα was positively correlated with the M2 distribution of 13.7% and Treg distribution of 12.9%, while the rest were explained by other factors. Conclusion: TNFα has a positive correlation with M2 and Treg distribution,but mostly through a different mechanism other than EBER-TLR3 interaction. Possibly, other pro-inflammatory and anti-inflammatory cytokines are involved in the formation of the NPC microenvironment, especially related to the presence of M2 and Treg, which provide immunosuppressive effects in NPC tumors. <br /> https://journal.waocp.org/article_89703_12aec146133fdd46aca72dc19e609592.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736822820210801Young Breast Cancer: Novel Gene Methylation in WBC237123758972710.31557/APJCP.2021.22.8.2371ENMehrdadNoruziniaDepartment of Medical Genetics, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.JavadTavakkoly-BazzazDepartment of Medical
Genetics, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.MohamadAhmadvandHematology, Oncology, and Stem Cell
Transplantation Research Center, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran.FatemehAzimiEye Research Center,
the Five Senses Institute, Rassoul Akram Hospital, Iran University of Medical Sciences, Tehran, Iran.0000-0001-9995-0142AliDehghanifardDepartment of Vaccination,
Pasteur Institute of Iran, Tehran, Iran.GolnazKhakpourEye Research Center,
the Five Senses Institute, Rassoul Akram Hospital, Iran University of Medical Sciences, Tehran, Iran.Journal Article20210407Introduction: Breast cancer is a highly diverse disease, and epigenomic alterations, as principle changes in the pathogenesis of breast cancer, have recently been noticed in epimarker research on peripheral blood. Methods: In this study, DNA samples isolated from the white blood cells of 30 breast cancer patients were compared to 30 healthy controls using methylated DNA immunoprecipitation microarray (MeDIP-chip) to determine differentially methylated region as a potential epimarker in cancer and control cases. Results: A total of 1799 differentially methylated regions were identified, including ZNF154, BCL9, and HOXD9, in which significant methylation differences were confirmed in breast cancer patients through a quantitative real-time polymerase chain reaction. Differential methylation of the mentioned genes has been reported in different cancer tissues and cell-free DNA, including breast cancer. Methylation of those genes listed in the white blood cells of our young patients not only relates to their importance in the pathogenesis of breast cancer but may also highlight their potential as primary epimarkers that can warrant further evaluation in large cohort studies. It is important to note that methylation alteration in WBC, as well as genetic mutation, can be identified years before cancer development, which emphasizes this issue as a potential screening marker.https://journal.waocp.org/article_89727_a9f16a4f85ee189812fe290c29b8db09.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736822820210801Assessment of Practice and Outcome of Exercise on Quality of Life of Women with Breast Cancer in Delta State237723838970410.31557/APJCP.2021.22.8.2377ENLinda COdikpoDepartment of Nursing Science, Nnamdi Azikiwe University Awka, Nnewi Campus, Nigeria.0000-0003-4438-204XEdith NChiejinaDepartment of Nursing Science, Nnamdi Azikiwe University Awka, Nnewi Campus, Nigeria.Journal Article20201211Objectives: This study evaluated the effect of exercise on the quality of life of women with breast cancer. It determined the pre and post-intervention functional, symptoms, and global quality of life of women with breast cancer in the control and intervention groups. Methods: The quasi-experimental design study adopted a purposive sampling technique in selecting the women with breast cancer in the intervention (47) and control (47) groups. Data on QOL was measured using standardized instruments, namely the European Organization for Research and Treatment of Cancer Quality of Life Questionnaire-version 3 (EORTC QLQ-C30. Results: The results show the pre-intervention overall functional quality of life domain score was 59.2±21.98 for the intervention and 67.0± 20.13 for the control, an overall score for the symptom domain was 31.8±10.89 for the intervention and 29.8±10.24 for the control. The post-intervention overall generic functional domain score was 89.0±11.1 for the intervention and 51.2±17.8 for the control and for symptoms domain; their overall score was 16.4±10.2 for the intervention and 35.4±12.4 for the control. A significant difference existed in all post-intervention functional domain scores of the generic function and symptoms quality of life as the functional domains (p < 0.001) and global health status (p < 0.001). Conclusion: As the numbers of women who survive breast cancer continue to increase, there is a need for lifestyle modification like exercise to improve and maintain their overall QOL to live a fulfilled life devoid of post-treatment complications.https://journal.waocp.org/article_89704_abd855f2a98f170e76d7d8256f34f7ac.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736822820210801100 Disruptive Publications in Breast Cancer Research238523898973410.31557/APJCP.2021.22.8.2385ENMiles WGrunvaldDepartment of Surgery, Rush University Medical Center, Chicago, IL, 60654, USA.https://orcid.org/00Michael DWilliamsDepartment of Surgery, Rush University Medical Center, Chicago, IL, 60654, USA.Ruta DRaoDepartment of Hematology and Oncology, Rush University Medical Center, Chicago, IL, USA.Cristina MO’DonoghueDepartment of Surgery, Rush University Medical Center, Chicago, IL, 60654, USA.Adan ZBecerraDepartment of Surgery, Rush University Medical Center, Chicago, IL, 60654, USA.0000-0002-6072-3775Journal Article20201217Background: Breast cancer has a rich history of research over the past 75 years. Many studies have had disruptive influences on the field itself. Our study employs a new, validated measurement to determine the most disruptive publications within the field of breast cancer. Materials and Methods: PubMed® database was queried for articles between 1954-2014 related to breast cancer with in 21 different journals deemed important to the field. Articles were then scored for disruption and citation count. The top 100 most disruptive and cited publications were compiled and analyzed. Results: Disruption score was a distinct measurement from citation count and had low level of correlation. Disruptive publications tended to skew older with the median year of publication in 1977. The score identified a variety of study designs and publication types within multiple journals. Conclusions: Measurement of the disruptive quality of a publication is a new way to describe academic impact of a publication and is distinct from citation count. Used in conjunction with citation count in may give a more descriptive bibliometric assessment of the literature. Further exploration within the field of oncology is warranted.<br /> https://journal.waocp.org/article_89734_d368b2ebbdb1764526aa46b14d9591dc.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736822820210801Immune Dysfunction as Measured by the Systemic Immune-Inflammation Index is Associated with the Sub-Type of Minimal Residual Disease and Outcome in Stage II Colon Cancer Treated with Surgery alone239123978970510.31557/APJCP.2021.22.8.2391ENNigel PMurrayFaculty of Medicine, Finis Terrae University, Av. Pedro de Valdivia 1509, Providencia, Santiago, 7501015, Chile.0000-0001-8154-8550RicardoVillalonColoproctology
Service, Hospital de Carabineros de Chile, Simón Bolívar 2200, Ñuñoa, Santiago, 7770199, Chile.0000-0002-8341-7658ShendaOrregoFaculty of Medicine, University
Mayor, San Pio X 2422, Providencia, Santiago, Chile.0000-0003-2860-2954EghonGuzmanFaculty of Medicine, University
Mayor, San Pio X 2422, Providencia, Santiago, Chile.0000-0001-5012-6945Journal Article20210113Objective: Within 5 years after curative surgery for stage II colon cancer 25% of patients will relapse due to minimal residual disease (MRD). MRD is the net result of the biological properties of subpopulations of primary tumour cells which enable them to disseminate, implant in distant tissues and survive and the immune system’s ability to eliminate them. We hypothesize that markers of immune dysfunction such as the systemic inflammation index (SII) are associated with the sub-type of MRD defined by bone marrow micro-metastasis (mM) and circulating tumour cells (CTCs). A higher immune dysfunction being associated with a more aggressive MRD and worse prognosis. Methods and Patients: Blood and bone marrow samples were taken to detect CTCs and mM using immunocytochemistry with anti-CEA one month after surgery. The SII, absolute neutrophil, platelet and lymphocyte counts (ANC, APC, ALC) were determined immediately pre-surgery and one month post-surgery. These were compared with the sub-types of MRD; Group I MRD (-); Group II mM positive and Group III CTC positive; cut-off values of SII of >700 and >900 were used. Follow-up was for up to 5 years or relapse and survival curves using Kaplan-Meier (KM) were calculated. Results: One hundred and eighty one patients (99 women) participated, mean age 68 years, median follow up 4.04 years; I: = 105 patients, II: N= 36 patients, III: N=40 patients. The SII significantly decreased post-surgery only in Group I patients. The frequency of SII >700 and >900 was significantly higher in Group III, between Groups I and II there was no significant difference. The SII was significantly associated with the number of CTCs detected. The 5-year KM was 98% Group I, 68% Group II and 7% Group III. Conclusions: The results of the study suggest that the severity of immune dysfunction as determined by the SII is associated with differing sub-types of MRD and a worse prognosis; increasing immune dysfunction is associated with a more aggressive CTC positive MRD sub-type; a more severe immune dysfunction is associated with a higher number of CTCs detected.<br /> https://journal.waocp.org/article_89705_759f852b3b94dfc9029dd7e813b732c5.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736822820210801Physical Activity, Sitting Time, and Quality of Life among Breast and Gynaecology Cancer Survivors239924088970610.31557/APJCP.2021.22.8.2399ENNadzirah HanisZainordinNutritional Sciences Programme, Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, Kuala Lumpur, Malaysia.Center for Community Health Studies (ReaCH), Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, Kuala Lumpur, Malaysia.0000-0001-5133-1561NorimahA. KarimDepartment of Nutrition and Dietetics, School of Health Sciences, International Medical University, Bukit Jalil, Kuala Lumpur, Malaysia.Mohd RazifShahrilNutritional Sciences Programme, Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, Kuala Lumpur, Malaysia.Center for Healthy Ageing and Wellness (H-CARE), Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, Kuala Lumpur, Malaysia.0000-0002-8680-3087RuzitaAbd TalibNutritional Sciences Programme, Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, Kuala Lumpur, Malaysia.Center for Community Health Studies (ReaCH), Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, Kuala Lumpur, Malaysia.0000-0002-3174-7065Journal Article20210126Background: Increasing physical activity and reducing sitting time was recommended to cancer survivors after cancer treatment for sustained health and to enhance the quality of life. This study aimed to determine the association of physical activity and sitting time with quality of life among the Malay breast and gynaecological cancer survivors. Methods: A cross-sectional study was conducted among 95 breast and gynaecology cancer survivor subjects. The Malay International Physical Activity Questionnaire (IPAQ) was used to assess physical activity and sitting time. Quality of life was assessed using the Malay EORTC QLQ-C30 questionnaire. Sociodemographic, clinical characteristics and anthropometric measurements were also obtained in this study. Results: The mean age of the subject was 51.8 ± 7.7 years old and the duration of survivorship was 4.3 ± 3.4 years. A total of 76.8% of subjects were categorized as having low physical activity level with a mean MET 403.5 ± 332.7 minutes/week and sitting time of 416.9 ± 151.0 minutes/day. Overall, subjects aged 50 years and above (p=0.006), widowed (p=0.032), retired (p=0.029) and had other non-communicable diseases (p=0.005) showed lower levels of physical activity. Increased physical activity had a positive effect on physical function (r=0.2, p=0.038), reduced insomnia (r=-0.3, p <0.001) and constipation symptoms (r=-0.3, p=0.012) domains of quality of life. The longer the sitting period showed more severe insomnia symptoms (r=0.2, p=0.03) but improved social function (r=0.2, p=0.012). Conclusions: Increasing physical activity and reducing sitting time have a positive effect on the quality of life of cancer survivors. The focus of health education should be prioritized to older adults (50 years and above), widows, retirees, and those with other comorbidities as they are at risk of being not physically active.<br /> https://journal.waocp.org/article_89706_bbc4f153b3dd617b94dce1394cbb6e4c.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736822820210801Molecular Docking Compounds of Cinnamaldehyde Derivatives as Anticancer Agents240924198970710.31557/APJCP.2021.22.8.2409ENWarsitoWarsitoFaculty of Mathematic and Natural Sciences, Essential Oil Institute, Brawijaya University, Malang, Indonesia.ShintaMurlistyariniLaboratory of Biomedic, Faculty of Medicine, Brawijaya University, Malang, Indonesia.SuratmoSuratmoFaculty of Mathematic and Natural Sciences, Essential Oil Institute, Brawijaya University, Malang, Indonesia.Vina OAzzahraFaculty of Mathematic and Natural Sciences, Essential Oil Institute, Brawijaya University, Malang, Indonesia.AndrianSucahyoFaculty of Mathematic and Natural Sciences, Essential Oil Institute, Brawijaya University, Malang, Indonesia.Journal Article20210212Objective: Cinnamaldehyde (CM) has a molecular structure with the main reaction center of an aromatic ring which the bioactivity can be modified as an anticancer agent by substituting the groups in the ortho (o), meta (m), and para (p) position. The present study aimed to investigate the correlation of the cluster region that was substituted in CM on its activity for various anticancer receptors. Methods: The receptor types used in the test were 5FL6, 1HOV, 4GY7, 5EAM, 4XCU, 4EL9, and 4PQW. The suitability of the hydroxy (OH) and methoxy (OMe) groups, which were substituted, was studied based on the value of Ki, their interactions with metal cofactors, and the type of amino acid residues that function as cancer receptor inhibitors. The docking was conducted using AutoDock 4. Results: The study results showed that all derivative compounds (o, m, and p) –OH and –OMe CM commonly had better anticancer activities than CM. o-OH CM has the best activity against receptors 5FL6, 1HOV, 4GY7, 5EAM, and 4XCU, and m-OMe CM has better activity against the 4EL9 receptors when compared with other CM derivatives. Conclusion: Based on this study, the compound derived from CM, i.e. OHC, tends to show the best anticancer activity.<br /> https://journal.waocp.org/article_89707_5973f23455fc7bdf1dc4870139b9c253.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736822820210801Awareness Level of Cancer Risk Factors and Warning Signs and Cancer Campaign Attendance behavior among Saudi Adults in a Tertiary Hospital in Riyadh242124288970810.31557/APJCP.2021.22.8.2421ENEssa MSabiDepartment of Pathology, Clinical Biochemistry Unit, Collage of Medicine , King Saud University, Riyadh, Saudi Arabia.0000-0001-8266-5177Ahmed H AMujamammiDepartment of Pathology, Clinical Biochemistry Unit, Collage of Medicine , King Saud University, Riyadh, Saudi Arabia.MoathAbdulghaniCollege
of Medicine, King Saud University, Riyadh, Saudi Arabia.Yasser MAlmesferCollege
of Medicine, King Saud University, Riyadh, Saudi Arabia.Anas AAlsuwaidaCollege
of Medicine, King Saud University, Riyadh, Saudi Arabia.Abdullah SulimanBalobaidCollege
of Medicine, King Saud University, Riyadh, Saudi Arabia.Abdulaziz FahadAlangariCollege
of Medicine, King Saud University, Riyadh, Saudi Arabia.Abdulrahman SaudAlharbiCollege
of Medicine, King Saud University, Riyadh, Saudi Arabia.Journal Article20210213Objective: To estimate the awareness level of Saudi adults about the risk factors and warning signs of cancer and observe the association of different determinants with cancer Knowledge in Riyadh city. Methods: A cross-sectional study carried out on 390 Saudis in the outpatient clinics of King Khalid University Hospital (KKUH), Riyadh. Data was collected using a validated Arabic questionnaire. Data was analyzed using SPSS software. Results: The overall awareness of the participants was limited, as the mean score of the overall cancer knowledge was 49.2%. Most of the correctly answered questions were on general cancer knowledge and not about risk factors or warnings signs. The only risk factors identified by most participants were smoking, alcohol, air pollution and genetic factors. Important risk factors such as physical inactivity, low dietary fibers and obesity were not well known. Despite Hepatitis B virus infection (HBV) being endemic in the kingdom, only 30% identified it as a risk factor. Apart from “A Thickening or a lump in breast or other organs”, < 50% of participants recognized alarming warning signs such as unexplained weight loss, unusual bleeding, and change in bowel habits. Females were more inclined to attend cancer awareness campaigns. Though people who reported attendance of cancer awareness campaigns surprisingly did not achieve a significantly higher overall knowledge score. Conclusion: The public lacks knowledge of well-established cancer risk factors and warning signs, despite recent advances in the medical field. Results suggest that current strategies to educate the public need to be revised.<br /> https://journal.waocp.org/article_89708_b900ed8b2c0362b089899f533faac52a.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736822820210801High Expression of Stem Cell-Related Genes in Polyps with Villous Features and High-Grade Dysplasia Support Malignant Phenotype and Colorectal Carcinogenesis242924358973510.31557/APJCP.2021.22.8.2429ENIbrahimSahinDepartment of Medical Genetics, University of Health Sciences, Dışkapı Yıldırım Beyazıt Training and Research Hospital,
Ankara, Turkey.Department of Medical Genetics, Faculty of Medicine, Ataturk University, Erzurum, Turkey.0000-0002-6050-816XBetülGündoğduDepartment of
Medical Pathology, Faculty of Medicine, Ataturk University, Erzurum, Turkey.Ahmet CevdetCeylanDepartment of Medical Genetics, Faculty of
Medicine, Ankara Yildirim Beyazit University, Ankara, Turkey.Haktan BagisErdemDepartment of Medical Genetics, University of Health Sciences,
Ankara Dr. Abdurrahman Yurtaslan Oncology Training and Research Hospital, Ankara, Turkey.AbdulganiTatarDepartment of Medical Genetics, Faculty of Medicine, Ataturk University, Erzurum, Turkey.Journal Article20210219Objective: The aim of this project is to identify the differences in expression levels of stem cell related genes (SCRGs) in normal colon tissue, histopathologically staged colon polyps and colon cancer, and to explain the role of SCRGs in the formation of CC and for contributing the practical usage of SCRGs in the diagnosis and treatment of CC. Methods: Normal colon tissue, hyperplastic polyps, histopathologically (HGD and LGD) staged tubular, tubulovillous and villous polyps and colon cancer paraffin tissue (FFPE) samples were used. Transcription factor genes (OCT4, KLF4, SOX2, MYC, NANOG, and REX1) and cell surface markers (CD133, LGR5), which are associated with embryonic stem cells, induced pluripotent stem cells, and cancer stem cells, have been selected for measuring expression levels from the selected tissues. After isolation of total RNA from FFPE tissues, SCRGs expressions were measured by RT-qPCR method. Results: SCRGs expression differences were detected in normal–adenoma–cancer progression. A significant increase was observed in the expression of LGR5 (p: 0.01) and PROM1 (p: 0.005) genes in villous HGD polyps, LGR5 (p: 0.003) gene in G1, and LGR5 (p: 0.0002) and MYC (p: 0.002) genes in G2 stage tumor tissues. When compared with each other, a significant increase in SCRGs expression is noticeable in the formation from adenoma to cancer tissues regarding malign phenotype. Conclusion: This study shows that the increase of SCRGs expressions occurs with high-grade dysplasia (HGD), villous features, and the malignant phenotype. Increased expression levels of LGR5, PROM1, KLF4, SOX2, and MYC in HGD and cancerous tissues support the malignant phenotype and the existence of cancer stem cells and demonstrate that they can be used to assess diagnosis and prognosis. Identification of tissue-specific SCRGs expressions will help design new therapies to control the development and progression of colonic neoplasia.https://journal.waocp.org/article_89735_959a5066297db213aa9db359d1740db6.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736822820210801Prevalence of Trismus and Its Impact on Oral Health-Related Quality of Life in Patients Treated for Oral Squamous Cell Carcinoma243724448971010.31557/APJCP.2021.22.8.2437ENShailesh MGondivkarDepartment of Oral Medicine & Radiology, Government Dental College & Hospital, Nagpur, Maharashtra, India.Amol RGadbailDepartment
of Dentistry, Indira Gandhi Government Medical College & Hospital, Nagpur, Maharashtra State, India.0000-0002-6546-7694Sachin CSarodeDepartment of Oral
Pathology & Microbiology, Dr. D.Y. Patil Dental College & Hospital, Dr. D.Y. Patil Vidyapeeth, Pune, Maharashtra State, India.0000-0003-1856-0957SubhrajitDasguptaRST Cancer Hospital & Research Centre (Tertiary care cancer centre), Nagpur, Maharashtra State, India.BalkrishnaSharmaRST Cancer Hospital & Research Centre (Tertiary care cancer centre), Nagpur, Maharashtra State, India.AmolHedaooRST Cancer Hospital & Research Centre (Tertiary care cancer centre), Nagpur, Maharashtra State, India.AparnaSharmaDepartment of Dentistry,
Government Medical College & Hospital, Nagpur, Maharashtra State, India.Gargi SSarodeDepartment of Oral
Pathology & Microbiology, Dr. D.Y. Patil Dental College & Hospital, Dr. D.Y. Patil Vidyapeeth, Pune, Maharashtra State, India.MonalYuwanatiDepartment of Oral Pathology and Microbiology.
Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai,
India.0000-0001-9233-8292Rima SGondivkarDental Surgeon, , Aarti regency, Mahalakshmi Nagar, Manewada Road, Nagpur, Maharashtra State, India.ShankargoudaPatilDepartment
of Diagnostic Sciences, Division of Oral Pathology, College of Dentistry, Jazan University, Jazan, Kingdom of Saudi Arabia0000-0001-7246-5497Rahul NGaikwadDepartment of Community Dentistry and Oral Epidemiology, College of Dentistry, Qassim University, Buraydah, Kingdom of
Saudi Arabia.Journal Article20210226Oral squamous cell carcinoma, one of the most common malignancies, has a poor prognosis due to impairment in oral functions secondary to treatment. Trismus one of the major causes of impairment of oral function. The present study investigated the prevalence of trismus and its impact on oral health-related quality of life (OHRQoL) in patients treated for oral squamous cell carcinoma (OSCC). The maximum inter-incisal mouth opening of hundred OSCC patients was recorded at post-treatment and 3 months post-treatment. OHRQoL questionnaire (OHIP-14) was intervened to assess the OHRQoL of patients post-treatment and 3 months follow-up, with emphasis on correlation with grades of trismus. The prevalence of trismus was 16% pre-treatment, 72% post-treatment, and 62% at 3 months after treatment. The overall OHIP-14 scores indicated that patients with trismus reported greater impairment of OHRQoL than those without trismus at the end of treatment and 3 months follow-up. At the end of treatment, patients with severe trismus demonstrated a higher mean OHIP-14 score (23.47 ±3.34) than those with moderate (17.72 ±2.83) and mild trismus (12.66 ±3.84) with statistically significant differences (p <0.001). Equivalent results were obtained at 3 months follow-up period. Patients with trismus suffer greater impairment of OHRQoL. The findings demand the need of identifying risk factors for developing trismus and early institution of newer/modified treatment approaches for better OHRQoL in OSCC survivors.<br /> https://journal.waocp.org/article_89710_645156952aba91d3c218fd81b2e6b303.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736822820210801Prognostic Significance of Tripartite Motif Containing 16 Expression in Patients with Gastric Cancer244524518971110.31557/APJCP.2021.22.8.2445ENJalilAfsharDepartment of Biochemistry, Neyshabur Branch, Islamic Azad University, Neyshabur, Iran.JamshidMehrzadDepartment of Biochemistry, Neyshabur Branch, Islamic Azad University, Neyshabur, Iran.0000-0001-8700-3914HassanMehrad-MajdCancer Molecular Pathology Research
Center, Mashhad University of Medical Sciences, Mashhad, Iran.0000-0002-8745-6558LadanGoshayeshiDepartment of Gastroenterology and Hepatology, School of
Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.JafarSaeidiDepartment of Physiology, School of Basic Science, Neyshabur
Branch, Islamic Azad University, Neyshabur, Iran.Journal Article20210227Background and Aim: Tripartite Motif Containing 16 (TRIM16) is a member of the TRIM protein family which is known to play a suppressor role in development of numerous tumor types. However, a positive correlation between TRIM16 expression and gastric cancer (GC) progress has created a controversial situation that need to be fully delineated. The aim of this study was to assess the expression level of TRIM16 mRNA and its relationship with β-catenin, CyclinD, and BCL2 expression in Iranian GC patients and to investigate its possible association with patients’ overall survival. Materials and Methods: The expression level of TRIM16 of fresh primary tumor and adjacent normal tissues in 40 GC patients was evaluated by real-time quantitative PCR method. Moreover, patients were subdivided into high or low expression subgroups based on the TRIM16 expression levels. The relationship between TRIM16 expression level, β-catenin, Cyclin D, BCL2, some clinicopathological data and prognosis of GC patients was also analyzed. Results: qPCR analysis showed a lower level of TRIM16 in GC tissues (fold change=0.351) in comparison to their matched adjacent noncancerous tissues (p <0.001). Contrary to this, the expression levels of β-catenin, Cyclin D, and BCL2 genes were up-regulated in cancerous samples. This may explain the tumor suppressive function of TRIM16 in GC; as reduction in TRIM16 expression leads to the accumulation of mRNAs from β-catenin, Cyclin D, and BCL2 genes and eventually cancer progression. We did not observe any significant correlation between TRIM16 expression and patients’ overall survival. Univariate Cox regression analysis indicated that anemia, weight loss, bleeding, stomach ache, and smoking are statistically associated with overall survival; while, multivariate analysis did not support any correlation. Conclusions: In sum, this study suggests a tumor suppressive role for TRIM16 in gastric cancer and proposes it as a potential candidate for GC prognosis.<br /> https://journal.waocp.org/article_89711_b626be433b46498bc664837033b1407b.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736822820210801Guided imagery relaxation in quality of life of patients undergoing hematopoietic stem cell transplantation: a quasi-experiment245324608972810.31557/APJCP.2021.22.8.2453ENLuana Aparecida Alves DaSilvaComplex Hospital de Clinicas, Federal University of Parana, Brazil.0000-0003-0210-2791Celina Angélica MattosMachadoComplex Hospital de Clinicas, Federal University of Parana, Brazil.0000-0002-7241-7169Edenice De OliveiraSantanaErasto Gaertner Hospital, Brazil.0000-0001-8453-455XMariana Nunes DaSilvaFederal University of Paraná, Brazil.0000-0002-8610-2889Jorge Vinícius CestariFelixDepartment of Nursing, Federal University of Parana, Brazil.0000-0002-0086-674XNamie OkinoSawadaDepartment of Nursing, University of São Paulo, Brazil.0000-0002-1874-3481Paulo Ricardo BittencourtGuimarãesDepartment of Statistics, Federal University of Parana, Brazil.0000-0002-9852-6777Luciana PuchalskiKalinkeDepartment of Nursing, Federal University of Parana, Brazil.0000-0003-4868-8193Journal Article20210308Objective: The aim of this study was to evaluate the effects of the relaxation technique with guided imagery by means of virtual reality on health-related quality of life in patients undergoing hematopoietic stem cell transplantation. Methods: A quasi-experiment conducted in a Bone Marrow Transplantation Service of a public hospital in southern Brazil. From October 2019 to October 2020, forty-two adult participants who underwent transplantation were included, 35 in the intervention group and seven in the control group. A guided imagery intervention, with audio guiding the relaxation associated with nature images in 360º, was performed during the hospitalization period. Data were collected on the first day of hospitalization, on the transplantation day, during the neutropenia stage, and at pre-hospital discharge. The Functional Assessment of Cancer Therapy-Bone Marrow Transplantation (FACT-BMT), Functional Assessment of Chronic Illness Therapy-Fatigue (FACIT-Fatigue) and Functional Assessment of Cancer Therapy-Neutropenia (FACT-N) were used to assess health-related quality of life, fatigue and neutropenia. Data were analyzed using the Generalized Linear Mixed Model for the evolution of the health-related quality of life assessments over time, considering the groups and stages. Pearson’s correlation coefficient was adopted for the correlation analyses. Results: Allogeneic transplantation was predominant: 28 (80%) in the intervention group and 5 (71.43%) in the control group. There were improvements in the health-related quality of life scores, although not significant. A significant difference was found among the stages (p <0.050) and a significant positive correlation (p <0.000) among the variables on general quality of life, additional concerns, fatigue and neutropenia in all stages. Conclusion: Patients undergoing hematopoietic stem cell transplantation suffer changes in their quality of life. Interventions based on integrative practices emerge as an option to minimize them. <br /> https://journal.waocp.org/article_89728_a556d17372653af4cc154cf9b9d66665.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736822820210801Molecular Landscape and Computational Screening of the Natural inhibitors against HPV16 E6 Oncoprotein246124698972910.31557/APJCP.2021.22.8.2461ENTanzilJunejaDepartment of Microbiology, School of Science, RK University, Rajkot, India.https://orcid.org/00Medha DPandyaThe KPES Science College, M.K Bhavnagar
University, Bhavnagar, India.https://orcid.org/00SejalShahDepartment of Microbiology, School of Science, RK University, Rajkot, India.https://orcid.org/00Journal Article20210314Background: Human Papillomavirus (HPV) is a small, non-enveloped, icosahedral and double-stranded DNA virus with a genome of 8 kb, belonging to the papillomaviridae family. HPV has been associated with 99.7% cases of cervical squamous cell carcinoma worldwide. The HPV E6 protein is known as a potent oncogene and is closely allied with the events that result in the malignant transformation of virally infected cells. Objective: The present study aims to target plant derived anticancer molecules for HPV driven cancer using a computational approach. Methods: In this study, E6 oncoprotein was targeted by 101 plant-derived nutraceuticals using the molecular docking method. The multiple sequence analysis and phylogenetic analysis of low risk and high risk 28 HPV E6 proteins were performed. Results: Withanolide D, Ginkgetin, Theaflavin, Hesperidin, and Quercetin-3-gluconide were identified as the potential inhibitors of HPV 16 E6 protein. The zinc finger domain was identified on all variants of HPV E6 oncoprotein while high-risk HPV18, HPV31, HPV33, HPV35, HPV39, HPV45, HPV58, HPV68 and HPV73: probable risk HPV53 and low-risk HPV43 and HPV70 contain PDZ domain. Conclusion: The current study using bioinformatics analysis approaches reveals a promising platform for developing anti-cancerous competitive inhibitors targeting HPV.<br /> https://journal.waocp.org/article_89729_b31cf581e761e5d8cb6bec2da9159f4b.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736822820210801Teucrium polium Extract Enhances the Anti-Angiogenic Effect of Tranilast in a Three-Dimensional Fibrin Matrix Model247124788971210.31557/APJCP.2021.22.8.2471ENFatemehSheikhbahaeiDepartment of Anatomy, Afzalipour School of Medicine, Kerman University of Medical Sciences, Kerman, Iran.0000-0001-7416-2764Seyed NoureddinNematollahi-MahaniPhysiology Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran.0000-0002-4733-1953MozafarKhazaeiFertility
and Infertility Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran.0000-0003-0402-3333Mohammad RasoolKhazaeiFertility
and Infertility Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran.0000-0001-9818-0625SaeedKhazayelFertility
and Infertility Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran.Journal Article20210319Objective: Angiogenesis plays a dominant role in many pathophysiologic disorders, including cancer. Tranilast, which is an anti-fibrotic drug, is also suggested as an anti-angiogenesis agent. As Teucrium polium (TP) is known as an herbal medicine with antitumor properties, this study aimed to investigate the effects of TP and Tranilast on human umbilical vein endothelial cells (HUVECs), in vitro model of angiogenesis, as well as rat’s aortic ring ex vivo model. Methods: In this study, The HUVECs were treated with various doses of TP and Tranilast each one alone or in combination together. Cell survival test, aortic ring ex-vivo assay, and evaluating mRNA expressions of VEGFA and TGF-β ligands and receptors were performed. Results: The survival rate of HUVECs has significantly (p <0.05) reduced by TP and Tranilast. The combination of both TP and Tranilast significantly reduced cell viability as compared to the administration of TP or Tranilast alone. As well, the treatment of HUVECs with TP and/or Tranilast significantly (p <0.05) decreased TGF-β1, TGF-β 2, TGF-βRI, and TGF-βRII mRNA expression levels, but not the expression of TGF-β3 and TGF-βRIII in the TP-treated cells. Image analysis showed that TP and/or Tranilast inhibited vascular growth in the aortic ring assay. Conclusion: Our results strongly support the anti-angiogenic effects of the TP and Tranilast combination on both in vitro and ex vivo models of angiogenesis. However, further investigations in in vivo models and human studies are needed before human use.https://journal.waocp.org/article_89712_94ace03710885441f4c3fca70721e0c5.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736822820210801Modifying Effect of Smoking on GSTM1 and NAT2 in Relation to the Risk of Bladder Cancer in Mongolian Population: A Case-Control Study247924858971310.31557/APJCP.2021.22.8.2479ENShiirevnyambaAvirmedDivision of Urology, School of Medicine, Mongolian National University of Medical Sciences, Ulaanbaatar, Mongolia.Department of Graduate Studies, Graduate School, Mongolian National University of Medical Sciences, Ulaanbaatar, Mongolia.0000-0002-1010-8221YerkhanatKhuanbaiDivision of Urology, School of Medicine, Mongolian National University of Medical Sciences, Ulaanbaatar, Mongolia.0000-0002-1341-999XAmarsaikhanSanjaajamtsDivision of Urology, School of Medicine, Mongolian National University of Medical Sciences, Ulaanbaatar, Mongolia.0000-0001-6528-2510BaasansurenSelengeCenter of Urology and Andrology, First Central Hospital of Mongolia, Ulaanbaatar, Mongolia.0000-0003-2136-3129Bayan-UndurDagvadorjCenter of Urology and Andrology, First Central Hospital of Mongolia, Ulaanbaatar, Mongolia.MakotoOhashiInstitute of Arts and Sciences, Tokushima University, Japan.0000-0002-6655-0238Journal Article20210325Objectives: Tobacco smoking is the predominant risk factor for bladder cancer as it contains cancer-causing chemicals. However, genetic factors may play important role in response towards chemical carcinogens. In this study we aim to investigate genetic polymorphisms of glutathione S-transferase M1 (GSTM1) and N-acetyltransferase 2 (NAT2) as determinants of bladder cancer risk, independently and in combination with tobacco use in the Mongolian population. Materials and Methods: The current study was a hospital-based case-control study including 60 histologically confirmed bladder cancer patients and 60 cancer-free controls. PCR-RFLP assay was used to determine the presence of GSTM1 and NAT2 polymorphisms in bladder cancer patients and controls. GSTM1 and NAT2 were tested using binary logistical regression analysis with adjustment or stratification according to the smoking. Results: There were 46 men and 14 women diagnosed with bladder cancer, with mean age was 58±4. The controls included 37 men and 23 women with a mean age of 57±3. The frequency of GSTM1 null genotype was higher in controls (71.67%) than in bladder cancer patients (58.33%) without statistical significance (OR=0.5534; 95% CI=0.2586-1.1843), (p=0.128). The NAT2 low acetylator phenotype was more common in patients with bladder cancer (15%) than in controls (5%). Furthermore, individuals with NAT2 low acetylator phenotype had a nearly 3.35-fold increased risk to develop bladder cancer (OR=3.35; 95% CI=0.8604-13.0657), (p=0.081) while the risk was even higher when combined with null GSTM1 genotype (OR=4; 95% CI=0.4459-37.5308), (p=0.213) but there was no statistical significance. Prevalence of smoking in bladder cancer patients was higher than controls and increased significantly the risk of bladder cancer (OR=8.31; 95% CI=3.66-18.88). Smokers with GSTM1 null genotype were at 5-fold higher risk of bladder cancer (OR=5.0; 95% CI=1.55-16.16), (p=0.007) while NAT2 low acetylator phenotype increased bladder cancer risk by 20-fold (OR=20.5; 95% CI=2.33-80.86), (p=0.006). Conclusion: The current study shows that tobacco smokers with the NAT2 low acetylator phenotype and GSTM1 null genotype have the highest risk of bladder cancer in the Mongolian population.<br /> https://journal.waocp.org/article_89713_b032977e1813ffd6411e92e22111c5f5.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736822820210801Reliability and Validity of the Brief Illness Perception Questionnaire in Bahasa Malaysia for Patients with Cancer248724928973310.31557/APJCP.2021.22.8.2487ENHarenthri Devy AlagirRajahFaculty of Health Science, Universiti Kebangsaan Malaysia, Campus Kuala Lumpur, Kuala Lumpur, Malaysia.Faculty of
Arts and Social Science, University Tunku Abdul Rahman, Kampar, Perak, Malaysia.Qui TingChieFaculty of Medicine, Universiti Malaya,
Kuala Lumpur, Malaysia.MahadirAhmadFaculty of Health Science, Universiti Kebangsaan Malaysia, Campus Kuala Lumpur, Kuala Lumpur, Malaysia.0000-0003-1550-5688Wun ChinLeongFaculty of Health Science, Universiti Kebangsaan Malaysia, Campus Kuala Lumpur, Kuala Lumpur, Malaysia.Department of Radiotherapy and Oncology, National Cancer Institute, Putrajaya, Malaysia.NirmalaBhoo-PathyFaculty of Medicine, Universiti Malaya,
Kuala Lumpur, Malaysia.Caryn Mei HsienChanFaculty of Health Science, Universiti Kebangsaan Malaysia, Campus Kuala Lumpur, Kuala Lumpur, Malaysia.0000-0002-5916-4363Journal Article20210325Objective: The main purpose of this study was to identify the psychometric properties and validate the Bahasa Malaysia version of the Brief Illness Perception Questionnaire among patients with cancer. Methods: A total of 129 newly diagnosed patients with cancer were consecutively sampled. Reliability and validity of the questionnaire were tested using translation validity, test-retest reliability, Principal Component Analysis, Cronbach’s alpha coefficient for domains and item-total correlation. Results: The questionnaire indicates excellent test-retest reliability. The Principal Component Analysis (PCA) revealed that Kaiser-Meyer-Olkin (KMO) is 0.60 for the two-factor structure of the Brief Illness Perception Questionnaire of the Bahasa Malaysia version which consists of cognitive illness representation and emotional illness representation. Conclusion: The Brief Illness Perception Questionnaire in the Bahasa Malaysia version is a useful tool to use among patients with cancer in Malaysia context despite moderate psychometric properties. This is based on the premise that the questionnaire can be used as a quick tool to assess illness perceptions among Malaysian with cancer in routine oncology practice.https://journal.waocp.org/article_89733_0f82c94da72e81a4f154dbe8fb5c5fce.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736822820210801Effect of Lysyl Oxidase G473 A Polymorphism on Lysyl Oxidase and Total Soluble Collagen Expression in Oral Submucous Fibrosis249324998972210.31557/APJCP.2021.22.8.2493ENSanjitMukherjeeCSIR-Indian Institute of Chemical Biology, Kolkata, India.0000-0002-3295-9668AtulKatarkarCSIR-Indian Institute of Chemical Biology, Kolkata, India.RichaDhariwalDepartment of Oral Pathology, Dr. R. Ahmed Dental College and Hospital, Kolkata, India.SwetaMohantyDepartment of Oral Pathology, Dr. R. Ahmed Dental College and Hospital, Kolkata, India.BasudevMahatoDepartment of Oral Pathology, Dr. R. Ahmed Dental College and Hospital, Kolkata, India.Jay GopalRayDepartment of Oral Pathology, Dr. R. Ahmed Dental College and Hospital, Kolkata, India.KeyaChaudhuriCSIR-Indian Institute of Chemical Biology, Kolkata, India.Journal Article20210405Background: Oral submucous fibrosis (OSF) is a debilitating collagen-metabolic disorder leading to submucosal fibrosis and trismus. Lysyl oxidase (LOX), a critical collagen biosynthetic enzyme, is up-regulated in OSF. Polymorphisms in the Lysyl oxidase gene have been associated with increased risk of OSF and might affect normal collagen synthesis, accumulation, or degradation, crucial in determining fibrosis severity. Methods: One hundred OSF cases and 100 controls were genotyped for LOX G473A(Arg158Gln) polymorphism by polymerase chain reaction-restriction fragment length polymorphism. The expression of LOX was estimated both by quantitative mRNA analysis and western blot. Total soluble collagen was evaluated from mucosal tissue obtained from OSF cases. Immunohistochemical (IHC) localization of type 1 collagen was performed in mucosal tissue obtained from patients carrying various genotypes. Results: Heterozygous G473A genotype was significantly higher in OSF cases [2.063(95% CI =1.059-4.016)], among 26-40 years age-group [4.375(95% CI=1.323-14.267),p=0.029] and in male patients [2.38 (95% CI= 1.107-5.121), p= 0.042]. LOX expression was significantly higher in cases of the heterozygous or homozygous carrier (p <0.001). We found the total soluble collagen level significantly (p <0.001) higher among patients carrying GA or AA genotype. IHC revealed focal deposition of type1 collagen in the submucosal tissue; comparatively higher deposition was evident in mucosal tissue of OSF patients carrying AA genotype. Conclusions: These findings suggest LOX G473A polymorphism confers an increased risk of OSF and may affect collagen accumulation in OSF cases.https://journal.waocp.org/article_89722_4c3c98586af376a525ec434f75574a5d.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736822820210801Lung Cancer Survival with Current Therapies and New Targeted Treatments: A Comprehensive Update from the Srinagarind Hospital-Based Cancer Registry from (2013 to 2017)250125078971410.31557/APJCP.2021.22.8.2501ENWachirapornMusikaBueng Khong Long Hospital, Bueng Khong Long District, Bueng Kan Province, Thailand.SupotKamsa-ArdDepartment of Epidemiology and Biostatistics, Faculty of Public Health, Khon Kaen University, Khon Kaen, Thailand.ASEAN Cancer Epidemiology and Prevention Research Group, Khon Kaen University, Khon Kaen, Thailand.0000-0002-1554-7696ChananyaJirapornkulDepartment of Epidemiology and Biostatistics, Faculty of Public Health, Khon Kaen University, Khon Kaen, Thailand.ASEAN Cancer Epidemiology and Prevention Research Group, Khon Kaen University, Khon Kaen, Thailand.ChalongponSantongCancer Unit, Srinagarind Hospital, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.AnakapongPhunmaneeDepartment of Medicine, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.Journal Article20210408Background: Lung cancer (LC) is a common malignancy and leading cause of cancer death worldwide and in Thailand. An update on LC survival factors after diagnosis at Srinagarind Hospital is needed. Methods: We conducted a retrospective cohort study, and the data were sourced from the Srinagarind Hospital-Based Cancer Registry. All LC cases were diagnosed between January 1, 2013, and December 31, 2017, and followed up until November 30, 2019. Cases of LC (ICD-O-3) numbered 2,149, but only those with coding C34.0-C34.9 were included. The survival rate was estimated using Kaplan-Meier, while the Log-rank test was used to estimate survival. Hazard ratios (HRs) and 95% confidence intervals (CIs) were estimated using Cox proportional hazard regression models. Results: The 2,149 patients had a total follow-up of 269.6 person-years. Overall, 1,867 patients died during the study, for a corresponding case-fatality mortality rate of 86.0 per 100 person-years. The respective 1-, 3-, and 5-year survival rate was 31.2 % (95% CI; 29.21 to 33.15%), 12.9 % (95%CI: 11.49 to 14.45), and 10.2% (95%CI: 8.74 to 11.70). After patient diagnosis, the median survival time was 0.46 years (5.51 months) (95% CI: 0.42 to 0.50). Targeted therapy was associated with longer survival than non-targeted therapy (p-value < 0.001). After adjusting for sex, TNM stage, and histologic type, multivariable analysis of the entire cohort identified chemotherapy as an independent predictor of improved survival (adjusted HR= 0.48; 95% CI: 0.42 to 0.55; P < 0.001), and that sex, TNM stage, and histologic type were associated with survival. Conclusion: The study confirmed that sex, stage of disease, histology, and chemotherapy are associated with survival of LC. Primary prevention and screening for early detection improve survival. Further investigations into factors affecting survival of LC in Northeast Thailand should focus on targeted therapy.<br /> https://journal.waocp.org/article_89714_8e3a8b83ea21479ba59f21de7fcff5f1.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736822820210801Serum Human Epididymis Protein-4 (HE4) - A novel Approach to Differentiate Malignant Frombenign Breast Tumors250925078971510.31557/APJCP.2021.22.8.2509ENK S SSai BabaDepartment of Biochemistry, Nizam’s Institute of Medical Sciences, Hyderabad, India.0000-0001-9047-0636Mohd AbdulRehmanDepartment of General Surgery, Osmania General Hospital, Hyderabad, India.JPradeep KumarDepartment of Biochemistry, Nizam’s Institute of Medical Sciences, Hyderabad, India.MairaFatimaDepartment of Biochemistry, Nizam’s Institute of Medical Sciences, Hyderabad, India.G S NRajuDepartment of Surgical Oncology, Nizam’s Institute of Medical Sciences, Hyderabad, India.Shantveer GUppinDepartment of Pathology, Nizam’s Institute of Medical Sciences, Hyderabad, India.NoorjahanMohammedDepartment of Biochemistry, Nizam’s Institute of Medical Sciences, Hyderabad, India.0000-0002-2545-7379Journal Article20210410Background: The lack of sensitivity and specificity of existing diagnostic markers like Carbohydrate Antigen 15-3(CA15-3) and Carcinoembryonic antigen (CEA) in breast cancer stimulates the search for new biomarkers to improve diagnostic sensitivity especially in differentiating benign and malignant breast tumors. Expression of Human epididymal protein 4 (HE4) has been demonstrated in ductal carcinoma of the breast tissue. So we tried to evaluate serum HE4 levels as diagnostic marker in breast cancer patients and to comparatively assess serum HE4, CEA and CA15-3 in breast tumor patients both benign and malignant. Methods: Total 90 female subjects were included in the study. We selected 30 breast cancer cases (Malignant group) and 30 benign breast lump cases (Benign group) based on histopathology report. And other 30 were age matched apparently healthy controls (Control group). HE4, CEA and CA15-3 were analysed in serum samples of all subjects by Electrochemiluminiscence immunoassay method. Results: A significant difference in the median (IQR) of HE4 (pmol/l) was identified among malignant, benign and control groups {62.4(52.6-73.7) vs 49.3(39.8-57.4) vs 52.3(50.6-63.3) P=0.0009} respectively. The cutoff value for prediction of breast cancer was determined at >54.5 pmol/l for HE4, with a sensitivity of 73.3%, specificity of 65.3%, whereas cutoff value of CA 15-3 was >21.24 (U/ml) with a sensitivity of 56.7%, specificity of 74.5%. For CEA at cutoff value >0.99 (ng/ml) the sensitivity and specificity were 96.7 % and 62.7% respectively. AUC for HE4, CA15-3 and CEA were 0.725, 0.644 and 0.857 respectively. Conclusion: Our study demonstrated that serum levels of HE4 were significantly higher in malignant group compared to benign and control groups. There is no significant difference between HE4 levels between benign and control groups. These results indicate that HE4 appears as a useful and highly specific biomarker for breast cancer, which can differentiate between malignant and benign tumors.https://journal.waocp.org/article_89715_f93581353e362e6ba616e5d2287dd985.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736822820210801Protective Factors against Depressive Symptoms in Female American Indian Cancer Survivors: The Role of Physical and Spiritual Well-being and Social Support251525208971610.31557/APJCP.2021.22.8.2515ENSoonheeRohDepartment of Social Work, University of South Dakota, State of South Dakota, USA.0000-0002-4387-7211Yeon-ShimLeeSchool of Social Work, San Francisco State University, State of California, USA.0000-0002-9394-2827Yi-PingHsiehDepartment of Social Work, University of North Dakota, State of North Dakota, USA.Scott DEastonSchool of Social Work, Boston College, State of Massachusetts, USA.Journal Article20210416Background: This exploratory study examined how perceived physical well-being, spiritual well-being and social support relate to depressive symptoms among American Indian (AI) female cancer survivors. Methods: Cross-sectional data were obtained from 73 AI female cancer survivors between 32 to 77 years of age in the Midwest of the United States. Standardized measures were used to assess for depression (Center for Epidemiologic Studies Depressive Symptoms Scale Short Form), spiritual well-being (Functional Assessment of Chronic Illness Therapy, Spiritual Well-being Scale), and social support (Medical Outcomes Study of Social Support Questionnaire). A single item measured physical well-being. After univariate and bivariate analyses, hierarchical multiple regression (HMR) was used for modeling. Results: Approximately 47% of the sample scored higher than 10 on the depressive symptoms scale. HMR results indicated that perceived physical well-being, spiritual well-being, and social support were negatively associated with depressive symptoms, accounting for 47% of the variance in the final model (adjust R2 = 0.47). Conclusions: A high percentage of the sample exceeded the cut point for probable depression, highlighting the importance of addressing mental health in the aftermath of cancer treatments for AI women. Results suggest that perceived physical well-being, spiritual well-being, and social support serve as protective factors and possible levers to reduce depression in this population. Interventions designed to bolster existing social support and spirituality could improve the mental health of AI women in the aftermath of cancer treatment. Community-based, culturally appropriate health education programs should be developed to enhance AI women’s physical health. <br /> https://journal.waocp.org/article_89716_524945c8b6d1427ae3d769b716616f6d.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736822820210801Comparisons of Factors Influencing Cancer Rescreening Intention in Middle-Aged and Elderly252125288971710.31557/APJCP.2021.22.8.2521ENHeejungKimCollege of Nursing, Research Institute of Nursing Science, Daegu Catholic University, Republic of Korea.Journal Article20210420Objective: This study was conducted to identify and compare factors that influence the intention of middle-aged and elderly in Korea to undergo cancer screening. Methods: The participants were 367 middle-aged individuals aged 40 to 59 and elderly older than 60 years who had been undergoing cancer screening in D-city, Korea. Data were collected using structured questionnaires that asked about knowledge, health beliefs, and satisfaction regarding cancer screening as well as cancer re-screening intention. The data were analyzed using the SPSS/WIN 25.0 program. Results: Factors influencing cancer re-screening intentions differed between middle-aged and elderly populations. The influencing factors of cancer re-screening intention among middle-aged were perceived benefits, perceived barriers, and perceived severity of cancer screening, while among the elderly perceived benefits, experience with cancer screening, and the reason for cancer screening were identified. Conclusions: The results indicate that a customized and systemic approach considering the characteristics of middle-aged and elderly is needed to promote consistent cancer screening in middle-aged and elderly. <br /> https://journal.waocp.org/article_89717_68d529659b658d472a17ec39f4bd8eec.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736822820210801Anticancer Effect of Rh2, a Histone Deacetylase Inhibitor, in HepG2 Cells and HepG2 Cell-Derived Xenograft Tumors Occurs via the Inhibition of HDACs and Activation of the MAPK Signaling Pathway252925398973610.31557/APJCP.2021.22.8.2529ENShi QingQiangThe Second Affiliated Hospital of Chongqing Medical University, Chongqing, P.R. China.Laboratory of Stem Cell and Tissue
Engineering, Department of Histology and Embryology, Chongqing Medical University, Chongqing, China.Gao ChaoQinDepartment of
Pharmacy, Renhe Community Health Service Center of Chongqing Liangjiang New Area, Chongqing, China.LiJingLaboratory of Stem Cell and Tissue
Engineering, Department of Histology and Embryology, Chongqing Medical University, Chongqing, China.Feng ZiQiangLaboratory of Stem Cell and Tissue
Engineering, Department of Histology and Embryology, Chongqing Medical University, Chongqing, China.Qin HongMeiThe First People’s
Hospital of Chongqing Liangjiang New Area, Chongqing, China.Chen DiLongLaboratory of Stem Cell and Tissue
Engineering, Department of Histology and Embryology, Chongqing Medical University, Chongqing, China.Chongqing Three Gorges Medical College, Chongqing, P.R.
China.Journal Article20210428Purpose: To investigate the effect of 20(S)-ginsenoside Rh2 (Rh2) on anti HepG2 liver cancer cells and HepG2 cell-derived xenograft tumors, and explore the underlying mechanisms. Materials and Methods: The activity of total HDACs and HAT were assessed with a HDACs colorimetric kit. Expression of HDAC1, HDAC2, HDAC6, p-ERK, ERK, p-P38, P38, p-JNK and JNK proteins was tested by Western blotting.H3K9 and H3K14 proteins were also checked by immunofluorescence, changes in cell cycle distribution with flow cytometry, cell apoptosis with annexin V-FTIC/PI double staining. Activity of Renilla luciferase (HIF) was detected using the Luciferase Reporter Assay system reagent. Gene expression for CyclinD1, Bcl-2, Bax, HIF, IL-1, IL-6, IL-10 and TNF-α was tested by q-PCR. Expression levels of CD31 and Ki-67 was tested by immunohistochemical staining. Results: Total HDAC activity was decreased and total histone acetyltransferase (HAT)activity was increased in a time-dependent manner. Expression of HDAC1 and p-JNK proteins was significantly increased, expression levels of p-ERK was decreased. H3K9 and H3K14 fluorescence protein were increased. Flow cytometric analysis of the cell cycle revealed that the percentage of cells in the G0/G1 phase in the treatment group(64.35±1.36%) was significantly increased compared with the untreated group(61.61±1.23%).The apoptotic rate of the HepG2 group was 10.03±1.92%, which increased to 17.87±1.67% in the treatment group. Expression levels of the transcription factor HIF were also increased in HepG2 cells following induction by Rh2. Expression of CyclinD1 and Bcl-2 at the genetic level was significantly decreased, while expression levels of Bax, HIF, IL-1, IL-6, IL-10 and TNF-α was increased. In vivo, the expression levels of both CD31 and Ki-67 proteins were significantly down-regulated in the treatment group compared with the control group. Conclusions: The effects of Rh2 were suggested to occur through the inhibition of total HDAC activity, which subsequently induced MAPK signaling and down-regulated the expression of HIF.<br /> https://journal.waocp.org/article_89736_bb658f9b94ab6ef7f7253262d6614f59.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736822820210801Clinicopathological Prognostic Factors Influencing Survival Outcomes of Vulvar Cancer254125488971810.31557/APJCP.2021.22.8.2541ENMonwaneeMuangchangDivision of Gynecologic Oncology, Department of Obstetrics and Gynecology, Faculty of Medicine, Chiang Mai University,
Chiang Mai, Thailand.0000-0002-6324-2950PrapapornSuprasertDivision of Gynecologic Oncology, Department of Obstetrics and Gynecology, Faculty of Medicine, Chiang Mai University,
Chiang Mai, Thailand.0000-0002-0205-2280SurapanKhunamornpongDivision of Gynecologic Pathology, Department of Pathology, Faculty of Medicine, Chiang Mai University,
Chiang Mai, Thailand.0000-0002-5442-0188Journal Article20210501Background: The prognostic factors for survival in squamous cell carcinoma (SCCA) of vulva cancer such as groin node involvement, postmenopausal status, tumor size, margin status, tumor grade, lymph vascular space invasion (LVSI) were reported in the past. However, with limited data from Southeast - Asian population, the present study was conducted to evaluate the clinicopathological prognostic factors for survival outcomes of this disease after treatment with surgery. Methods: All SCCA vulva cancer patients who underwent surgery between January 2006 and December 2017 were reviewed. The clinicopathological factors were analyzed to identify the prognostic factors for the progression-free survival (PFS) and overall survival (OS) using the Kaplan- Meier method and Cox-Proportional Hazard model. Results: One hundred twenty-five patients were recruited. The independent poor prognostic factors for PFS were groin node-positive and pathologic tumor diameter of more than 25 mm. Whereas postmenopausal status and groin node positive were independent poor prognostic factors for OS. Conclusion: Groin node-positive was the only one independent poor prognostic factor for both PFS and OS. In addition, the tumor diameter longer than 25 mm. was independent poor prognostic factors for PFS while postmenopausal status was independent poor prognostic factors for OS. Special adjuvant treatment for patients with these factors should be further investigated. <br /> https://journal.waocp.org/article_89718_a5d6730182021dd15746749d98c9d977.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736822820210801Changes in Oral Function and Quality of Life in Tongue Cancer Patients Based on Resected Area254925578972310.31557/APJCP.2021.22.8.2549ENYoshiakiIharaDivision of Oral Rehabilitation Medicine, Department of Special Needs Dentistry, School of Dentistry, Showa University, Tokyo, Japan.0000-0002-4324-2670YuichiTashimoDivision of Oral Rehabilitation Medicine, Department of Special Needs Dentistry, School of Dentistry, Showa University, Tokyo, Japan.Head and Neck Oncology Center, Showa University Hospital, Tokyo, Japan.0000-0001-7961-2445ShinjiNozueDivision of Oral Rehabilitation Medicine, Department of Special Needs Dentistry, School of Dentistry, Showa University, Tokyo, Japan.YoshikiIizumiDivision of Oral Rehabilitation Medicine, Department of Special Needs Dentistry, School of Dentistry, Showa University, Tokyo, Japan.YumaFukunishiDivision of Oral Rehabilitation Medicine, Department of Special Needs Dentistry, School of Dentistry, Showa University, Tokyo, Japan.YoshiroSaitoHead and Neck Oncology Center, Showa University Hospital, Tokyo, Japan.ToshikazuShimaneHead and Neck Oncology Center, Showa University Hospital, Tokyo, Japan.KojiTakahashiDivision of Oral Rehabilitation Medicine, Department of Special Needs Dentistry, School of Dentistry, Showa University, Tokyo, Japan.Journal Article20210511Objective: Treatment of tongue cancer caused oral morbidities such as oral dryness, and dysphagia. The purpose of this study is to examine the time course of oral function and QOL based on resected area for patients after tongue cancer resection. Methods: 31 patients who underwent tongue cancer resection at the Showa University Head and Neck Oncology Center. The participants were divided into two groups; 24 participants in partial/hemi glossectomy group (PG), and seven in subtotal/total glossectomy group (TG). Participants were evaluated swallowing function (FOIS and MASA-C), tongue pressure (TP: kPa), BMI, whole body muscle mass (kg), and QOL evaluation (EORTC QLQ-C30, H & N35). Participants were measured at baseline (before surgical treatment), 1, 3, and 6 months after surgical treatment (1M, 3M, and 6M). Results: At baseline, tongue pressure and FOIS score of PG were significant higher than that of TG. At 1M, TP, MASA-C, and FOIS score of PG were significant higher than that of TG. At 3M, TP, MASA-C, and FOIS score of PG were significant higher than that of TG. At 6M, TP and MASA-C were significantly higher than that of TG. QOL measurements did not noted any significant difference between groups before 6M. At 6M, Some QOL measurements of TG related tongue function (Swallowing, Senses, Speech, Social contact) were significantly lower than PG. Conclusions: The resected area had significant effects on oral morbidities and feeding function. It is necessary to develop more effective rehabilitation methods to improve patients QOL who had functional impairment remained.<br /> https://journal.waocp.org/article_89723_85d765dea941adfcc6f32653d1e3393c.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736822820210801The Putative Role of Natural Killer Cells in Patients with Hepatitis C Virus-Related Hepatocellular Carcinoma255925678973710.31557/APJCP.2021.22.8.2559ENMona MHassounaDepartment of Clinical and Chemical Pathology, National Liver Institute, Menoufia University, Shebeen El-Kom, Egypt.Enas MRadwanDepartment of Clinical Pathology, National Cancer Institute, Cairo University, Cairo, Egypt.EmanAbdelsameeaHepatology and Gastroenterology,
National Liver Institute, Menoufia University, Shebeen El-Kom, Egypt.0000-0002-3225-7164SuzanneEstaphanDepartment of Physiology, Faculty of Medicine, Cairo
University, Cairo, Egypt.Heba EAbd ElrhmanDepartment of Clinical Pathology, Faculty of Medicine, Zagazig University, Egypt.MohamedAbdel-SamieeHepatology and Gastroenterology,
National Liver Institute, Menoufia University, Shebeen El-Kom, Egypt.0000 0002 8970 0286MaryNaguibDepartment of Clinical and Chemical Pathology, National Liver Institute, Menoufia University, Shebeen El-Kom, Egypt.Journal Article20210516Background: Natural Killer (NK) cells have crucial roles in immune responses against malignant transformation including hepatocellular carcinoma (HCC). The NKG2D receptor has a critical role in the NK recognition of target cells. Aim: We assessed NKG2D receptor expression as a diagnostic biomarker for HCC detection and progression in Egyptian patients with hepatitis C virus (HCV)-related HCC. Methods: We classified 81 patients into three groups: chronic hepatitis (21), cirrhotic (30) and HCC (30) patients, with 36 individuals enrolled to the control group. We analyzed NK levels in peripheral blood and NKG2D receptor expression in NK cells using flow cytometry. Results: We observed a significant decrease in NKG2D (CD314) expression on circulating NK cells and frequency of NK cells expressing NKG2D (CD314) in HCC patients. Also, in patients, larger foci lesions significantly correlated with decreased NK cell numbers. Multiple foci numbers and patients with a Child score C significantly correlated with decreased circulating NK cells expressing NKG2D and decreased NKG2D expression. Conclusion: The percentage of NK cells in peripheral blood and NKG2D receptor expression could function as potential biomarkers for HCC detection and progression.https://journal.waocp.org/article_89737_f7ea66e4c33ce42fd201b8f9f1575360.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736822820210801Cross-Sectional Study to Establish the Utility of Serum Tumor Markers in the Diagnosis of Lung Cancer256925768972410.31557/APJCP.2021.22.8.2569ENAnuragMehtaRajiv Gandhi Cancer Institute and Research Centre. India.AnujParkashRajiv Gandhi Cancer Institute and Research Centre. India.0000-0002-0758-6599MeenuBhatiaRajiv Gandhi Cancer Institute and Research Centre. India.Journal Article20210517Background: Reliable blood markers for aiding lung cancer (LC) diagnosis and differentiating LC from tuberculosis are lacking in India. Methodology: In this single-centre, cross-sectional, real-world study, serum levels of 5 TMs (CEA, CYFRA 21-1, SCC, ProGRP, NSE) were measured from consented patients with suspicious lung nodules who were candidates for biopsy, and also from healthy controls. TM level measurement was done through electrochemiluminescent immunoassay, followed by histological diagnosis on the biopsied specimen. Using package insert cut-offs, sensitivity and specificity of the 5 TMs were evaluated individually and in combination. Using receiver operating characteristic (ROC) curves of individual TMs, the ability of CEA, CYFRA 21-1, and ProGRP taken together was evaluated for its ability to differentiate LC from no-LC. Results: Out of 178 subjects, 160 had LC (147 NSCLC; 13 SCLC). NSCLC patients had higher median values of CYFRA 21-1 and SCC; SCLC patients had higher median values of CEA, NSE, and ProGRP. Adenocarcinoma-NSCLC patients had higher median values of CEA, CYFRA 21-1, NSE, and ProGRP; squamous-NSCLC patients had higher median value of SCC. For differentiating LC from no-LC, the combination of all 5 TMs (sensitivity:97.5%, specificity:33.3%) and combination of CEA, CYFRA 21-1 and ProGRP (sensitivity:91.3%, specificity:88.9%) were found suitable. Conclusion: Combination of all 5 TMs, and combination of CEA, CYFRA 21-1, and ProGRP represents an easy and non-invasive method for aid in LC diagnosis that does not require technical expertise. TM evaluation can also supplement histological diagnosis of LC. <br /> https://journal.waocp.org/article_89724_91b53dc7b057cff5098188674cbf1ace.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736822820210801Verification of Surface Dose for Flattening Filter and Flattening Filter Free Beams in Beam-Matched Medical Linear Accelerators257725858972510.31557/APJCP.2021.22.8.2577ENNSushmaDepartment of Radiation Physics, Kidwai Memorial Institute of Oncology, Bengaluru, India.Division of Medical Physics, JSS Academy of Higher Education and Research, Mysuru, India.ShanmukhappaKaginelliDivision of Medical Physics, JSS Academy of Higher Education and Research, Mysuru, India.SathiarajPalanivelDepartment of Radiation Physics, Kidwai Memorial Institute of Oncology, Bengaluru, India.K.MGaneshDepartment of Radiation Physics, Kidwai Memorial Institute of Oncology, Bengaluru, India.Journal Article20210517Background: The purpose of this study was to evaluate the surface dose (SD) of 6 and 10 MV flattening filter beam (FF) and flattening filter free (FFF) beam for different square field sizes in three Beam-matched medical linear accelerators using a parallel-plate ionization chamber. Materials and Methods: The experiment was carried out in a phantom composed of 40×40 cm2 solid Water slabs of varying thickness. Further sheets of solid water phantom were added to take readings in the build-up region for both SSD and SAD technique. Surface doses are measured with a PPC-05 chamber and DOSE 1 electrometer, at measurement depth of 1 mm interval and all results are plotted relative to the dose measured at Dmax for various field sizes. Surface dose readings are therefore reported as relative surface dose. Results: Surface dose increased linearly with field size for both FF and FFF photon beams in all three beam-matched linear accelerators in both SSD and SAD setup. The surface dose of FFF was higher than FF beams in all field sizes. For the given energy the surface dose difference (relative to 10x10 cm2 field size of 6FF) between FF and FFF beam was larger for large field size. For 6FF and 6FFF beam the surface dose difference for 5x5 cm2 is -5.27%, and for 30x30 cm2 it is 12.91%. The measured surface dose differences between linear accelerators are not statically significant (P>0.989). Similarly, the surface dose difference between SSD and SAD setup was also analysed and had no statistical significance (P>0.849). Conclusion: Study showed that the surface dose difference between beam-matched linear accelerators are insignificant. The surface dose difference between SSD and SAD setup were also found negligible. Most importantly, changing patients between beam-matched linear accelerators will not have any significant changes in surface dose in clinical setup.<br /> https://journal.waocp.org/article_89725_b2220863c185d256cbb9a1d97ca87068.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736822820210801Anticancer Potential of Silibinin Loaded Polymeric Nanoparticles against Breast Cancer Cells: Insight into the Apoptotic Genes Targets258725968973110.31557/APJCP.2021.22.8.2587ENAliPourgholiDepartment of Pharmaceutical Chemistry, Faculty of Pharmacy, Eastern Mediterranean University, Famagusta, TR North Cyprus, Turkey.MehdiDadashpourStem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.Department of Biotechnology, School of Medicine, Semnan University of Medical Sciences, Semnan, Iran.0000-0003-3352-7880AkramMousapourStem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.AkramFirouzi AmandiDepartment of Immunology, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.NosratollahZarghamiDepartment of Clinical Biochemistry and Laboratory Sciences, Faculty of Medicine, Tabriz, Iran.Department of medicine, Faculty of medicine, Istanbul Aydin University, Istanbul, Turkey.Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.Journal Article20210517Silibinin (SIL) is a natural polyphenolic flavonoid with multiple biological and anti-cancer features. However, the complex hydrophobic nature and inadequate bioavailability of SIL hinder its efficiency at tumor sites. Investigating the possibility of an extensive strategy for better treatment of breast cancer, we carried out a comparative exploration of the inhibitory effect of SIL and SIL loaded PLGA-PEG nanoparticle (SIL-NPs) on the expression of the proapoptotic target genes, which is considered as an influential molecular target for treatment of breast cancer. The main diameter of SIL-NPs was 220 ± 6.37 and 150 ± 23.14 nm via DLS and FE-SEM respectively. Furthermore, the zeta potential of PLGA-PEG and SIL-NPs was -5.48±0.13 and -6.8±0.26 mV respectively. SIL encapsulation efficiency and drug release were determined by about 82.32 % by analyzing the calibration curve of SIL absorbance at 570 nm. Cytotoxicity of SIL and SIL-NPs was conducted by MTT assay after 24, 48, and 72 h of exposure times, and the gene expression levels of apoptotic genes, p53 and hTERT was measured by real-time PCR. Evaluation of drug toxicity revealed that SIL-NPs represents higher cytotoxic effects than pure SIL in a time and dose-dependent manner. Moreover, the results demonstrated that SIL-NPs could induce apoptosis in breast cancer cells by upregulation of caspase-3, caspase-7, p53 and Bax, along with Bcl-2, hTERT, survivin and Cyclin D1 down regulation. Our results indicated that PLGA-PEG can be used as stable carriers in nano-dimensions and SIL-NPs can be considered as a promising pharmacological agent for cancer therapy.<br /> https://journal.waocp.org/article_89731_effa3b5a1803d20675f8fbdd96a4dd5b.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736822820210801Thyroid Nodule Size as a Predictor of Malignancy in Follicular and Hurthle Neoplasms259726028972610.31557/APJCP.2021.22.8.2597ENArunnitBoonrodDepartment of Radiology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.Radiology Informatics Lab,
Department of Radiology, Mayo Clinic, Rochester, MN, USA.0000-0001-6168-7668ZeynettinAkkusRadiology Informatics Lab,
Department of Radiology, Mayo Clinic, Rochester, MN, USA.M. ReginaCastroDepartment of Endocrinology, Mayo Clinic, Rochester, MN USA.AtefehZeinodiniRadiology Informatics Lab,
Department of Radiology, Mayo Clinic, Rochester, MN, USA.KennethPhilbrickRadiology Informatics Lab,
Department of Radiology, Mayo Clinic, Rochester, MN, USA.MariusStanDepartment of Endocrinology, Mayo Clinic, Rochester, MN USA.DanaEricksonDepartment of Endocrinology, Mayo Clinic, Rochester, MN USA.BradleyEricksonRadiology Informatics Lab,
Department of Radiology, Mayo Clinic, Rochester, MN, USA.Journal Article20210526Introduction: The management of follicular (FN) and Hurthle cell neoplasms (HCN) is often difficult because of the uncertainty of malignancy risk. We aimed to assess characteristics of benign and malignant follicular and Hurthle neoplasms based on their shape and size. Materials and methods: Patients with Follicular adenoma (FA) or carcinoma (FC) and Hurthle Cell adenoma (HCA) or carcinoma (HCC) who had preoperative ultrasonography were included. Demographic data were retrieved. Size and shape of the nodules were measured. Logistic regression analyses and odds ratios were performed. Results: A total of 115 nodules with 57 carcinomas and 58 adenomas were included. Logistic regression analysis shows that the nodule height and the patient age are predictors of malignancy (p-values = 0.001 and 0.042). A cutoff value of nodule height ≥ 4 cm. produces an odds ratio of 4.5 (p-value = 0.006). An age ≥ 55 year-old demonstrates an odds ratio of 2.4-3.6 (p-value = 0.03). Taller-than-wide shape was not statistically significant (p-value = 0.613). Conclusion: FC and HCC are larger than FA and HCA in size, with a cutoff at 4 cm. Increasing age increases the odds of malignancy with a cutoff at 55 year-old. Taller-than-wide shape is not a predictor of malignancy.https://journal.waocp.org/article_89726_2be7c49b969dd6680437e0810cb4a067.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736822820210801Tumor-Derived Matrix Metalloproteinase-13 (MMP-13) Expression in Benign and Malignant Breast Lesions260326098973210.31557/APJCP.2021.22.8.2603ENFereshtehAmeliDepartment of Pathology, Cancer Institute, Imam Khomeini Hospital Complex,Tehran University of Medical Sciences, Tehran, Iran.Department of Pathology, Faculty of Medicine, Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia.FirouzehGhafourina NassabDepartment of Pathology, Faculty of Medicine, Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia.NoraidahMasirDepartment of Pathology, Faculty of Medicine, Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia.FarzanKahtibDepartment of Electrical Engineering, Mashhad Branch, Islamic Azad University, Mashhad, Iran.Journal Article20210526Introduction: Breast carcinoma is the most common malignancy and the leading cause of cancer death in women. Matrix metalloproteinase-13 (MMP-13) is a hypothetical prognostic marker in invasive breast cancer. This study aimed to determine MMP-13 expression in benign and malignant breast lesions and to evaluate the correlation between MMP-13 expression and tumor characteristics in invasive ductal carcinoma (IDC). Materials and Method: We evaluated cytoplasmic expression of MMP-13 based on staining index using immunohistochemistry (IHC) in epithelial cells, stromal fibroblasts of IDC (n=90) and benign epithelial breast (n=90) lesions. Correlation between IHC and tumor size, lymph node status, distance metastasis, estrogen receptor (ER), progesterone receptor (PR) and Her-2/neu was assessed. Results: MMP-13 expression was 45% and 38.8% in malignant epithelial cells and peritumoral fibroblasts, respectively. Only low level of MMP-13 expression was seen in benign breast lesions (8.8% in epithelial component and 2.2% in stromal fibroblasts), while high level of MMP-13 expression was noted in malignant tumors, mainly grade II or III. Cytoplasmic MMP-13 expressions in epithelial tumor cells was correlated significantly with peritumoral fibroblasts. MMP-13 expression was directly correlated with distant metastasis and tumor stage in epithelial tumoral cells and was inversely correlated with progesterone expression in both tumoral and stromal cells. Conclusion: This study showed that MMP-13 was a moderator for tumor invasion and metastasis and could be an independent predictor of poor prognosis in breast cancer. The role of MMP-13 in predicting the risk of malignant transformation in benign lesions should be further investigated.https://journal.waocp.org/article_89732_37f3f138d6e6d9cd7ef841ca47a44cbb.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736822820210801Survival of Cancer Patients with Co-Morbid Tuberculosis in Thailand270127088973010.31557/APJCP.2021.22.8.2701ENPallopSiewchaisakulFaculty of Public Health, Chiang Mai University, Thailand.ASEAN Cancer Epidemiology and Prevention Research Group, Khon Kaen, Thailand.0000-0003-4738-1915SirinyaNanthanangkulASEAN Cancer Epidemiology and Prevention Research Group, Khon Kaen, Thailand.Udonthani Cancer Hospital, Medical Service Department, Ministry of Public Health, Thailand.0000-0001-9860-9268ChalongponSantongCancer Unit, Srinagarind Hospital, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.KrittikaSuwanrungruangASEAN Cancer Epidemiology and Prevention Research Group, Khon Kaen, Thailand.Cancer Unit, Srinagarind Hospital, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.PatravootVatanasaptASEAN Cancer Epidemiology and Prevention Research Group, Khon Kaen, Thailand.Cancer Unit, Srinagarind Hospital, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.Department of Otorhinolaryngology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.0000-0001-6037-8994Journal Article20210708Background and objective: We aimed to investigate the survival time and its related factors among cancer patients with co-morbid tuberculosis (TB) in Thailand. Methods: We conducted this retro-prospective cohort study on cancer patients without co-morbid TB using the data from population–based cancer registry of Khon Kaen, TB databases from the Khon Kaen Central Hospital, and the Region 7 Office of Disease Prevention and Control from 2001 to 2015 to determine the onset of TB after cancer. The cancer patients were then followed up until 2017 to assess their survival status. The Kaplan-Meier method, log-rank test, and Cox proportional hazard regression were used to estimate cumulative survival curves, compare various survival distributions, and adjusted hazard ratios. Results: Lung, head and neck, and liver cancers led to a significantly different survival time between patients with and without co-morbid TB. After adjustment, it was found that patients suffering from lung, head and neck, or liver cancer and co-morbid TB had significantly lower risk of death than those without co-morbid TB. Based on the stratified analysis, lung cancer patients with distant metastasis and co-morbid TB had 3.01-fold and 2.99-fold significantly increased risk of death compared to those without co-morbid TB. Conclusion: We found that cancer patients with co-morbid TB were at lower risk of death compared with those without co-morbid TB. In addition to cancer stage, it seems that cancer comorbidity with TB could modify the risk of death for lung cancer patients.There is a need for further studies to support our findings including other related risk factors. <br /> https://journal.waocp.org/article_89730_759865b5cd300b6008d23d5321ec9f12.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736822820210801Evaluating the Performance of Hybrid Capture 2 Test as a Primary Screening Test from Studies Conducted in Low and Middle-Income Country Settings- Special Focus India270927168973910.31557/APJCP.2021.22.8.2709ENKavita VAnandHomi Bhabha National Institute, Mumbai. India.Sharmila APimpleDepartment of Preventive Oncology, Tata Memorial Centre, Mumbai. India.AtanuBhattacharjeeDepartment of Biostatistics, Centre for Cancer Epidemiology, Tata Memorial Centre. Mumbai. India.Gauravi AMishraDepartment of Preventive Oncology, Tata Memorial Centre, Mumbai, India.Surendra SShastriDepartment of Health Disparities Research, Division of Cancer Prevention & Population Science, M.D Anderson Cancer Centre, Houston,USA.Journal Article20210113Among the screening tests for cervical cancer, advantages of screening with second generation molecular Hybrid Capture 2 (HC2) test is the high sensitivity and negative predictive value that makes it easy to implement as a cervical cancer screening policy necessitating less screening rounds. High income countries are now implementing HC2 test in their national cervical cancer screening program. Since the acceptance of any screening test depends on the sensitivity of the test, the current study was carried out to evaluate the sensitivity of HC2 test reported from Low- and Middle-income countries (LMIC) which share major burden of cervical cancer globally and to establish if HC2 test could be used as a primary screening test in India. Materials and methods: The population based cross sectional studies from LMICs which evaluated HC2 test as a primary screening modality to diagnose Cervical intraepithelial neoplasm grade 2 and above (CIN2+) lesions were included. Results: A total of 18 studies from LMIC involving 1,13,086 women were reviewed for sensitivity of HC2 as a primary screening test. The overall average sensitivity and specificity to diagnose CIN2+ lesions were 79.84% (95% CI-71.01,86.73) and 85.63% (95% CI- 84.37,86.92) respectively. India demonstrated an average sensitivity and specificity of 65% (95% CI 57,77) and 93% (95% CI- 92,94) respectively. Conclusion: Results from LMIC demonstrate a comparably low sensitivity of HC2 test to diagnose CIN2+ lesions as compared to that reported from High income countries. Sensitivity of HC2 was substantially low for India. The current study discusses issues of HC2 assay and the role of untreated Reproductive tract infections as probable causes for low sensitivity of the test. This needs further research in an attempt to improve the sensitivity of the test in an era of self-sampling and low-cost HPV test on horizon to improve the coverage for cervical cancer.<br /> https://journal.waocp.org/article_89739_7ebeebfd8b8116a18d0887034f82522b.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736822820210801Association of AXIN2 s2240308 C>T, rs1133683 C>T, rs7224837 A>G Polymorphisms with Susceptibility to Breast Cancer271727228974010.31557/APJCP.2021.22.8.2717ENSoheilaSayadDepartment of Surgery, Tehran University of Medical Sciences, Tehran, Iran.MahdiehAbdi-GamsaeDepartment of Biology, Shahr-e-Qods Branch,
Islamic Azad University, Tehran, Iran.JamalJafari-NedooshanDepartment of Surgery, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.MerajFarbodCancer Institute, Imam Khomeini Hospital, Tehran University of Medical Sciences, Tehran, Iran.Seyed AlirezaDastgheibDepartment of Medical Genetics, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.MojganKarimi-ZarchiDepartment of Obstetrics and Gynecology, Iran University of Medical Sciences, Tehran, Iran.Endometriosis Research Center, Iran University of Medical Sciences, Tehran, IranFatemehAsadianDepartment of Medical Laboratory Sciences, School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran.HosseinNeamatzadehDepartment of Medical Genetics, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.Mother and Newborn Health Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.Journal Article20210624Background: The association of genetic polymorphisms at Axis inhibition protein 2 (AXIN2) gene and susceptibility to different cancer has attracted much interest. The present study aimed to evaluate the association between AXIN2 rs2240308 C>T, rs1133683 C>T, rs7224837 A>G polymorphisms with susceptibility to breast cancer. Methods: A polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay was designed to genotype the AXIN2 rs2240308 C>T, rs1133683 C>T, rs7224837 A>G polymorphisms among 150 breast cancer patients and 150 healthy subjects. Results: The frequencies of these genetic variants were in agreement with Hardy-Weinberg equilibrium in healthy controls (p>0.05). The frequencies of AXIN2 rs2240308 C>T, rs1133683 C>T, rs7224837 A>G genotypes were similar in breast cancer patients and controls. There was no a significant association between the AXIN2 SNP and risk of breast cancer. Conclusion: The impact of AXIN2 polymorphisms in the breast cancer development remains unclear. Our results indicated that AXIN2 rs2240308, rs7224837 and rs1133683 polymorphisms did not contribute to increased risk of breast cancer. More studies with larger sample sizes and diverse ethnicities are warranted to verify our finding.https://journal.waocp.org/article_89740_987b56bf24b1fc1211ec97b487eeae81.pdf