Do TNFA -308 G/A and IL6 -174 G/C Gene Polymorphisms Modulate Risk of Gallbladder Cancer in North Indian Population?


Objectives: Gallbladder carcinoma (GBC) is highly aggressive neoplasm which arises in the background ofgall stones and inflammation. GBC affects women 2-3 times more frequently than men. Pro-inflammatory TNFAand IL6 gene polymorphism has been associated with various inflammatory diseases. The aim of this study wasto investigate whether TNFA -308 (G/A) and IL6 -174 G/C polymorphisms within flanking region of the genesare associated with GBC susceptibility.
Methods: The promoter polymorphisms were genotyped using PCRRFLPin 200 healthy subjects and 124 GBC patients.
Results: Frequency distribution of TNFA -308 (G/A) andIL6 -174 G/C were not significantly different in GBC patients in comparison to healthy controls. However,frequency of TNFA -308 (G/A) polymorphism in female GBC patients without gallstone were significantly different(p-value= 0.006) when compared to healthy female subjects (OR=3.054; 95% CI=1.39-6.72).
Conclusion: Theseresults suggest that TNFA -308 (G/A) polymorphism may influence the susceptibility of female gender gallbladdercancer in absence of gallstones while IL6 -174 G/C polymorphism does not seem to be playing significant role inthe susceptibility to gallbladder cancer.