We intended to study the mechanism of the inhibitory action of curcumin on human non-small cell lung cancerA549 cell. The cell growth was determined by CCK-8 assay, and the results indicated that curcumin inhibitedthe cell proliferation in a concentration dependent manner. And to further confirm the relative anti-cancermechanism of curcumin, RT-PCR was carried out to analysis the expression of relative apoptotic proteins Bax,Bcl-2. We found that curcumin could up-regulate the expression of Bax but down-regulate the expression ofBcl-2 in A549 cells. In addition, curcumin affect the mitochondrial apoptosis pathway. These results suggestedthat curcumin inhibited cancer cell growth through the regulation of Bcl-2/Bax and affect the mitochondrialapoptosis pathway.