Annexin A3 has been identified as a novel biomarker in different types of cancers. However, little is knownabout its clinical significances and and biological roles in gastric cancer. In this study, we assessed annexin A3expression in 80 patients with gastric cancer and explore its correlation with prognosis Moreover, correlationswith Ki-67, Bcl-2 and Bax were also investigated. Expression of annexin A3 was increased in gastric cancercompared with that in normal gastric tissues. Annexin A3 expression was significantly associated with tumorvolume and TNM stage (p<0.05). and inversely correlation with prognosis of patients. More interestingly,expression of annexin A3 was positive correlated with Ki-67 and Bcl-2 expression. Our study showed annexin A3might be a potential prognostic marker for gastric cancer and involved in tumorigenesis by regulating apoptosisand proliferation.