Diagnostic and Prognostic Value of Talin-1 and Midkine as Tumor Markers in Hepatocellular Carcinoma in Egyptian Patients

Document Type : Research Articles


1 Department of Biochemistry, Faculty of Pharmacy, Mansoura University, Mansoura, Egypt.

2 Department of Internal Medicine (Gastroenterology and Hepatology unit), Faculty of Medicine, Mansoura University, Mansoura, Egypt.

3 Oncology Center, Mansoura University, Mansoura, Egypt.


Background: Hepatocellular carcinoma (HCC) is a main cause of cancer death all over the world. Treatment and
outcome of HCC based on its early diagnosis. This study was conducted to estimate the role of talin-1 and midkine in
combination with total antioxidant capacity (TAC) as tumor markers in HCC patients. Methods: Serum levels of talin-1
and midkine were measured in 90 Egyptian subjects including 44 patients with HCC, 31patients with cirrhosis and 15
healthy controls using enzyme-linked immunosorbent assay (ELISA) technique. While a colorimetric method was used
for measurement of TAC. Results: Serum talin-1 in HCC patients was significantly lower than that in patients with
cirrhosis (P<0.001) and normal control (P<0.001). In addition, increased invasion and metastasis correlated with reduced
talin-1 level. Serum midkine in HCC patients was significantly higher compared to cirrhotic patients (P<0.001) and
normal control (P<0.001). Midkine at a cut off value of 1683 pg/ml showed a sensitivity of (81.82%) and specificity of
(83.87%). While alpha-fetoprotein (AFP) at a cut off value of 200 ng/ml had a sensitivity of (52.27%), while specificity
was (96.77%). Midkine was positive in 80.9% of HCC patients with negative AFP. Serum TAC was significantly
decreased in HCC patients when compared with control group (P<0.001). Conclusion: Talin-1 may be implicated
in the carcinogenesis and metastasis of HCC and can be used as a useful tumor marker for HCC. Midkine may be a
potential diagnostic marker for HCC and may be used in addition to AFP to increase the sensitivity of HCC detection.


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