Study on JV Virus in Patients with Colon Cancer Type Adenocarcinoma

Document Type: Research Articles

Authors

1 Infectious and Tropical Diseases Research Center Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

2 Virology Department, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

3 Alimentary Tract Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

4 Department of Pathology, Imam Khomeini Hospital, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

5 Department of Biostatistic, School of Health, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

Abstract

Colorectal cancer is the most repetitious malignancies with high mortality worldwide. JC virus (JCV) is ubiquitous
Polyomavirus, with seroprevalence rates ranging from 70% to 90% in adult population. Recently the role of JCV have
been reported in many malignant tumors worldwide. The association of JCV was reported in patients with colon and
rectum cancers. Thus this study was conducted to evaluate the association of JCV DNA in patients with colon cancer
type Adenocarcinoma. Material and Methods: A total of 120 formalin-fixed paraffin-embedded tissue blocks samples
were collected including 20/40(50%) males, 20/40(50%) females patients with Colorectal Cancer(CRC), and 80 (50%
males, 50% females) patients with benign tumor as a control. DNA was extracted for all the samples. Nested PCR was
carried out for detection of Vp1/T-Ag junction genome in JCV genome by Nested-PCR assay. Randomly, PCR products
of 6 samples were sequenced to analysis the partial JCV DNA. The phylogeny tree was constructed to determine
homology identity with other JCV. Results: 4/40(10%) samples of test group and 10/80 (12.5%) of control samples
were positive for JCV DNA (P= 0.69). Out of 4 samples positive for JC DNA, 3(7.5%) were males and 1(2.4%) female
(P=0.29). The frequency of JCV DNA in age group> 50 years was 4/32(10%), while in age group (0%) (p= 0.29). Conclusion: prevalence of JCV DNA was among 10% patients with CRC and 12.5% benign tumors
(p=0.69). The distribution of JCV DNA was among 7.5% male and 2.5% female (p= 0.29). The frequency of JCV
DNA was among 10% cases of age group >50 years and 0% of age group protein expression might explain the increased risk of colorectal cancer and requires further investigation.

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