Document Type : Research Articles
Post Graduate Program in Material Science, Federal University of Mato Grosso, Barra do Garças, MT, Brazil.
Institute of Biological and Health Science, Federal University of Mato Grosso, Barra do Garças, MT, Brazil.
Objective: To evaluate the antitumor effects of polyethylene glycol (PEG) microspheres with adsorbed Hancornia
speciosa ethanolic extract (HSEE) on blood mononuclear (MN) cells co-cultured with MCF-7 breast cancer cells.
Methods: PEG microspheres were adsorbed with HSEE and examined by flow cytometry and fluorescence microscopy.
MCF-7 and MN cells obtained from volunteer donors were pre-incubated alone or co-cultured (MN and MCF-7
cells) for 24 h with or without HSEE, PEG microspheres or PEG adsorbed with HSEE (PEG-HSEE). Cell viability,
superoxide release and superoxide dismutase were determined. Results: Fluorescence microscopy showed that PEG
microspheres were able to absorb HSEE throughout their surface. Irrespective of the treatment, the viability index of
MN cells, MCF-7 and their co-culture was not affected. Superoxide release increased in co-cultured cells treated with
HSEE, adsorbed or not onto PEG microspheres. In co-cultured cells, SOD levels in culture supernatant increased in
the treatment with HSEE, adsorbed onto PEG microspheres or not. Conclusion: HSEE has direct effects on MN cells
co-cultured with MCF-7 cells. The results suggest the benefits of Hancornia speciosa fruit consumption by women at
risk of breast cancer. In addition, because PEG-HSEE maintained oxidative balance in co-cultured cells, it is a promising
alternative for the treatment of tumor cells.